Biofilms and Antibiotic Resistance Determination from Different Immunocompetent Patients with Salmonellosis

Mariam Ayad Abd; Sawsan Q. T. Al-Quhli; Mahir Ali Jassim

doi:10.25163/angiotherapy.829502

Angiogenesis, Inflammation & Therapeutics | Online ISSN 2207-872X

RESEARCH ARTICLE (Open Access)

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Biofilms and Antibiotic Resistance Determination from Different Immunocompetent Patients with Salmonellosis

Mariam Ayad Abd ^1*, Sawsan Q. T. Al-Quhli ², Mahir Ali Jassim ³

+ Author Affiliations

Journal of Angiotherapy 8 (2) 1-11 https://doi.org/10.25163/angiotherapy.829502

Submitted: 15 December 2023 Revised: 14 February 2024 Published: 20 February 2024

Abstract

Background: Salmonellosis is an infectious disease due to presence of Salmonella enterica serovars, S. Typhi and S. Typhimurium. Our research demonstrated the antibiotic susceptibility, biofilm formation and isolation of Salmonella from patients. Methods: Biochemical tests and PCR analysis were employed to determine Salmonella isolates (83 isolates out of 217 samples) with the 16S rRNA gene detection. Microtiter plate assay was used to determine the biofilms formation on plastic surfaces. Results: 73.75% Salmonella was isolated from clinical samples. Antibiotic susceptibility showed that 85.18% of isolates from immunocompromised patients were sensitive to levofloxacin, and the rest were resistant to multiple antibiotics. 73.1% of S. Typhi were sensitive to levofloxacin among immunocompetent patients, and 69.23% were resistant to chloramphenicol. PCR test showed a rapid identification of Salmonella serovars. The results demonstrated the biofilm and isolates identification of Salmonella significantly. Increased multidrug resistance were observed from the results. Conclusion: The results showed Salmonella identification, antibiotic resistance, and molecular diagnosis strategy from clinical samples.

Keywords: Biofilm; Multidrug-resistant; Immunocompromised; Bacterial antibiotic susceptibility testing; Immunocompetent

References

Abbas, K. K., AL-Wattar, W. M. A., Hasan, S., Kasim, H., & Jasim, A. A. (2017). The incidence of Shigella and Salmonella in the stool of pediatric patients. Iraqi Journal of Public Health, 1(3).

Abdulah Y. Al-Mahdi, Alabed Ali A. Alabed, Mohammed Faez Baobaid et al., (2024). Antibacterial Activity of Herbal Essential Oils against Gram-positive and Gram-negative Bacteria with a Potential for Multidrug Resistance, Journal of Angiotherapy, 8(2), 1-7, 9517.

Akter, F., & Shamimuzzaman, M. (2023). Detection of invA Gene by PCR and Identification with Susceptibility Pattern Evaluation by VITEK 2 of Salmonella Isolates.

AL-Khafaji, J. K. T., AL-Yasari, H. F., & AL-Taei, M. H. (2006). Prevalence of typhoid fever among pediatric patients at Al-Musaib District. Medical Journal of Babylon, 3(1-2).

AL-Kraety, I. A. A., & Al-Ammar, M. (2017). relation of class1 integron gene with multidrug resistance salmonella typi isolates. Pak. J. Biotechnol. Vol, 14(4), 537-541.

AL-Taie, H. H. I. (2009). Prevalence rate of Salmonella in Babylon province. The Iraqi Journal of Veterinary Medicine, 33(2), 152-157.

Anderl, J. N., Franklin, M. J., & Stewart, P. S. (2000). Role of antibiotic penetration limitation in Klebsiella pneumoniae biofilm resistance to ampicillin and ciprofloxacin. Antimicrobial agents and chemotherapy, 44(7), 1818-1824.

Appiah, G. D., Mpimbaza, A., Lamorde, M., Freeman, M., Kajumbula, H., Salah, Z., Kugeler, K., Mikoleit, M., White, P. B., & Kapisi, J. (2021). Salmonella bloodstream infections in hospitalized children with acute febrile illness—Uganda, 2016–2019. The American Journal of Tropical Medicine and Hygiene, 105(1), 37.

Babu, D., Kalpana, K., & Juneja, V. K. (2015). Emergence of Drug-Resistant Pathogens. In Foodborne Pathogens and Food Safety (pp. 131-142). CRC Press.

Bukhari, E., Alaklobi, F., Bakheet, H., Alrabiaah, A., Alotibi, F., Aljobair, F., Alshamrani, M., Alaki, E., Alnahdi, F., & Alodyani, A. (2016). Disseminated bacille Calmette-Guérin disease in Saudi children: clinical profile, microbiology, immunology evaluation and outcome. European Review for Medical & Pharmacological Sciences, 20(17).

CDC. (2019). Salmonella. Retrieved from https://www.cdc.gov/salmonella/index.html

Cheesbrough, M. (2005). District laboratory practice in tropical countries, part 2. Cambridge university press.

Chen, S., Feng, Z., Sun, H., Zhang, R., Qin, T., & Peng, D. (2021). Biofilm-formation-related Genes csgD and bcsA promote the vertical transmission of Salmonella Enteritidis in chicken. Frontiers in Veterinary Science, 7, 625049.

CLSI : Clinical and Laboratory Standards Institute. Performance (2017). (27 ed.).

Dakorah, M. P. (2014). Prevalence of salmonella infections in patients attending St. Dominic hospital,(Akwatia)-Eastern Region

Ed-Dra, A., Filali, F. R., Khayi, S., Oulghazi, S., Bouchrif, B., El Allaoui, A., Ouhmidou, B., & Moumni, M. (2019). Antimicrobial resistance, virulence genes, and genetic diversity of Salmonella enterica isolated from sausages. European Journal of Microbiology and Immunology, 9(2), 56-61.

Fierer, J. (2022). Invasive non-typhoidal Salmonella (iNTS) infections. Clinical Infectious Diseases, 75(4), 732-738.

Flayyih, I. A. A. (2017). Molecular characterization of MDR Salmonella sp. Isolate from typhoid patients in Al. Najaf Al. Ashraf province-IRAQ University of Kufa].

Galanakis, E., Bitsori, M., Maraki, S., Giannakopoulou, C., Samonis, G., & Tselentis, Y. (2007). Invasive non-typhoidal salmonellosis in immunocompetent infants and children. International Journal of Infectious Diseases, 11(1), 36-39.

Gantois, I., Ducatelle, R., Pasmans, F., Haesebrouck, F., Gast, R., Humphrey, T. J., & Van Immerseel, F. (2009). Mechanisms of egg contamination by Salmonella Enteritidis. FEMS microbiology reviews, 33(4), 718-738.

Geddes, E., Collins, M., Abidi, S. S. R., Lees, J., Serrano, K. J., Gray, B., & Salmond, G. P. C. (2020). A comprehensive study of antibacterial activity of multi-metal doped SnO2 nanoparticles. Frontiers in Microbiology, 11, 348.

Halim, R. M. A., Kassem, N. N., & Mahmoud, B. S. (2018). Detection of biofilm producing staphylococci among different clinical isolates and its relation to methicillin susceptibility. Open access Macedonian journal of medical sciences, 6(8), 1335.

Hiba Ahmed Jawade, Zahraa Yosif Motaweq, Hawraa Dheyaa Rasool et al. (2024). Study of Antibiotic Resistance in ESKAPE Bacteria Using β-lactamase and ESBL Genes, Journal of Angiotherapy, 8(3), 1-8, 9618.

Hussaini I.M., Suleiman A.B. et al. (2021). Molecular Detection and Characterization of Carbapenemases Among Carbapenem-Resistant Escherichia Coli And Klebsiella Pneumoniae Isolated from Urine, Journal of Primeasia, 2(1), 1-6, 20212

Igomu, E. (2020). Salmonella Kentucky: prevalence and challenges in Nigeria and the Africa continent. African Journal of Clinical and Experimental Microbiology, 21(4), 272-283.

Le Thi Phuong, T., Rattanavong, S., Vongsouvath, M., Davong, V., Phu Huong Lan, N., Campbell, J. I., Darton, T. C., Thwaites, G. E., Newton, P. N., & Dance, D. A. (2017). Non-typhoidal Salmonella serovars associated with invasive and non-invasive disease in the Lao People’s Democratic Republic. Transactions of the Royal Society of Tropical Medicine and Hygiene, 111(9), 418-424.

Lim, S. H., Methé, B. A., Knoll, B. M., Morris, A., & Obaro, S. K. (2018). Invasive non-typhoidal Salmonella in sickle cell disease in Africa: is increased gut permeability the missing link? Journal of translational medicine, 16, 1-7.

MacKenzie, K. D., Palmer, M. B., Köster, W. L., & White, A. P. (2017). Examining the link between biofilm formation and the ability of pathogenic Salmonella strains to colonize multiple host species. Frontiers in Veterinary Science, 4, 138.

Muntaha R. Ibraheem, Dhafar N. Al-Ugaili. (2024). Nanoparticle-Mediated Plasmid Curing in Combating Antibiotic Resistance in Pathogenic Bacteria, Journal of Angiotherapy, 8(3), 1-8, 9495.

Nyabundi, D., Onkoba, N., Kimathi, R., Nyachieo, A., Juma, G., Kinyanjui, P., & Kamau, J. (2017). Molecular characterization and antibiotic resistance profiles of Salmonella isolated from fecal matter of domestic animals and animal products in Nairobi. Tropical Diseases, Travel Medicine and Vaccines, 3, 1-7.

Obaro, S. K., Hassan-Hanga, F., Olateju, E. K., Umoru, D., Lawson, L., Olanipekun, G., Ibrahim, S., Munir, H., Ihesiolor, G., & Maduekwe, A. (2015). Salmonella bacteremia among children in central and northwest Nigeria, 2008–2015. Clinical Infectious Diseases, 61(suppl_4), S325-S331.

Patel, J. B., Tenover, F. C., Turnidge, J. D., & Jorgensen, J. H. (2011). Susceptibility test methods: dilution and disk diffusion methods. Manual of clinical microbiology, 1122-1143.

SALIM, L., & MOTAWEQ, Z. Y. (2022). Phenotypically and genotypically estimation of virulence factors in Salmonella serovar typhi isolated from patients with enteric fever in Al-Najaf, Iraq. Nusantara Bioscience, 14(1).

Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: a laboratory manual. Cold spring harbor laboratory press.

Seixas, R., Machado, J., Bernardo, F., Vilela, C., & Oliveira, M. (2014). Biofilm formation by Salmonella enterica serovar 1, 4,[5], 12: i:-Portuguese isolates: a phenotypic, genotypic, and socio-geographic analysis. Current Microbiology, 68, 670-677.

Shaw, K. S., Cruz-Cano, R., Jiang, C., Baker, K., Dreyfuss, J. M., & M'ikanatha, N. M. (2021). Epidemiology and trends of salmonellosis in the USA, 1996–2016. Epidemiology and Infection, 149, e8.

S. M. Kamrul Islam, Maruf Abony et al. (2020). Prevalence of Multi-Drug Resistant (MDR) Pseudomonas sp. among patients attending in tertiary care hospital, Dhaka city, Bangladesh, Journal of Primesia University, 1(1), 1-8, 560011

Song, W., Shan, Q., Qiu, Y., Lin, X., Zhu, C., Zhuo, Z., Wang, C., Tong, J., Li, R., & Wan, C. (2022). Clinical profiles and antimicrobial resistance patterns of invasive Salmonella infections in children in China. European Journal of Clinical Microbiology & Infectious Diseases, 41(10), 1215-1225.

Sur, D., Ali, M., Von Seidlein, L., Manna, B., Deen, J. L., Acosta, C. J., Clemens, J. D., & Bhattacharya, S. K. (2007). Comparisons of predictors for typhoid and paratyphoid fever in Kolkata, India. BMC public health, 7(1), 1-10.

Tang, Y.-W., Stratton, C. W., & Aslanzadeh, J. (2006). Biochemical profile-based microbial identification systems. Advanced techniques in diagnostic microbiology, 84-116.

Ugboko, H., & De, N. (2014). Mechanisms of Antibiotic resistance in Salmonella typhi. Int J Curr Microbiol App Sci, 3(12), 461-476.

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