EMAN RESEARCH PUBLISHING | <p>Ethanolic Extract of <em>Triganella Foenum- grcacum L. </em>(Fenugreek) Mitigates Hypothyroidism-Induced Male Reproductive Dysfunction in Rats</p>

Inflammation Cancer Angiogenesis Biology and Therapeutics | Impact 0.1 (CiteScore) | Online ISSN 2207-872X

RESEARCH ARTICLE (Open Access)

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Ethanolic Extract of Triganella Foenum- grcacum L. (Fenugreek) Mitigates Hypothyroidism-Induced Male Reproductive Dysfunction in Rats

Fouad Ziedan Hamza Al-Quraishi ^1*, Bassam Abdulameer Mohammed Al-Madany ^1*, Mohammed Abdaljaleel ¹, Fadhel Al-Kraawi ¹, Zainab Mohammed Kadhim Hirzuldeen ¹, Karrar Talib Salman Al-Ibrahimi ¹

+ Author Affiliations

Journal of Angiotherapy 8 (6) https://doi.org/10.25163/angiotherapy.869684

Submitted: 08 April 2024 Revised: 03 June 2024 Published: 05 June 2024

Abstract

Background: Hypothyroidism, a common endocrine disorder, adversely affects various physiological processes, including male reproductive health. Fenugreek (Trigonella foenum-graecum L.), a traditional herbal remedy, is known for its therapeutic properties but also exhibits potential protective effects on reproductive function. This study aims to evaluate the protective effect of ethanolic extract of fenugreek seeds on sperm characteristics in hypothyroid male albino rats. Methods: Forty adult male albino rats (200–250 g) were divided into four groups, each containing ten rats. The negative control group received normal saline daily. Hypothyroidism was induced in the remaining three groups using 0.05% 6-propyl-thiouracil (PTU) for 30 days. Post-induction, the groups were treated as follows: the positive control group received normal saline, the third group received ethanolic extract of fenugreek seeds (15 mg/kg body weight), and the fourth group received levothyroxine (L-T4, 0.1 mg/kg/day). Hormonal levels of TSH, T3, and T4 were measured using the ELISA method, and sperm characteristics were analyzed from left caudal epididymis samples. Results: Hypothyroid positive control rats showed significantly increased TSH levels and decreased T3 and T4 levels compared to the negative control group. Treatment with either L-T4 or fenugreek extract significantly reduced TSH levels and increased T3 and T4 levels compared to the hypothyroid positive control group. Sperm analysis revealed that hypothyroidism significantly reduced sperm count, general motility, progressive motility, viability, and morphology. Both L-T4 and fenugreek treatments significantly improved all sperm parameters compared to the hypothyroid positive control group. Conclusion: Hypothyroidism impairs sperm characteristics, underscoring the importance of thyroid function in male fertility. The ethanolic extract of fenugreek seeds demonstrated a protective effect on sperm characteristics in hypothyroid rats, comparable to the effects of levothyroxine. These findings suggest that fenugreek may serve as a potential therapeutic agent for mitigating hypothyroidism-induced reproductive dysfunction. Further research is needed to explore the underlying mechanisms and clinical applications.

Keywords: Hypothyroidism, Fenugreek, Male fertility, Thyroid hormones, Sperm quality

References

Al-Habori, M., & Raman, A. (1998). Antidiabetic and hypocholesterolaemic effects of fenugreek. Phytotherapy Research, 12, 233-242.

Baby, B. T., Smitha, R., Remya, K., Shebina, P., & Azeem, A. K. (2016). Polycystic ovarian syndrome: Therapeutic potential of herbal remedies—a review. International Journal of Herbal Medicine, 4(5), 91-96.

Bashtian, M. H., Seyed, A. E., Nezhat, M., Habib Allah, E., Mahmoud, M., & Mahmoud, H. M. (2013). Evaluation of Fenugreek (Trigonella foenum-graecum L.) seeds extract effects on insulin resistance in women with polycystic ovarian syndrome. Iranian Journal of Pharmaceutical Research, 12(2), 475-481.

Biswas, N. M., Ghosh, P. K., Biswas, R., & Ghosh, D. (1994). Effect of thyroidectomy and thyroxin and α24-globulin replacement therapy on testicular steroidogenic and gametogenic activities in rats. Journal of Endocrinology, 140, 343-347.

Buzzard, J. J., Wreford, N. G., & Morrison, J. R. (2003). Thyroid hormone, retinoic acid and testosterone suppress proliferation and induce markers of differentiation in cultured rat Sertoli cells. Journal of Endocrinology, 144(9), 3722-3731.

François Xavier, K. N., Patrick Brice, D. D., Modeste, W. N., Esther, N., Albert, K., Pierre, K., & Pierre, W. (2019). Preventive effects of Aframomum melegueta extracts on the reproductive complications of propylthiouracil-induced hypothyroidism in male rats. Andrologia, 51(7), e13306.‏

Gu, L.-B., Liu, X.-N., Liu, H.-M., Pang, H.-L., & Qin, G.-Y. (2017). Extraction of Fenugreek (Trigonella foenum-graecum L.) seed oil using subcritical butane: Characterization and process optimization. Molecules, 22, 228.

Jiang, J.–Y., Umezu, M., & Sato, E. (2000). Characteristics of infertility and the improvement of fertility by thyroxin treatment in adult male hypothyroid rats. Biology of Reproduction, 63, 1637-1641.

Kaplan, E. L., Schwartz, S. I., Shires, G. T., Spencer, F. C., & Storer, E. H. (1984). Principles of surgery in Thyroid and parathyroid (4th ed.). McGraw-Hill, New York, pp. 1545-1635.

Kiss, R., Pesti-Asbóth, G., Szarvas, M. M., Stündl, L., Cziáky, Z., Hegedus, C., & Remenyik, J. (2019). Diosgenin and its fenugreek-based biological matrix affect insulin resistance and anabolic hormones in a rat-based insulin resistance model. BioMed Research International.‏

Kumar, A., Shekhar, S., & Dhole, B. (2014). Thyroid and male reproduction. Indian Journal of Endocrinology and Metabolism, 18, 23–31. https://doi.org/10.4103/2230-8210.126523

Kundu, S., Pramanik, M., Roy, S., De, J., Biswas, A., & Ray, A. K. (2006). Maintenance of brain thyroid hormone level during peripheral hypothyroid condition in adult rat. Life Sciences, 79, 1450–1455. https://doi.org/10.1016/j.lfs.2006.04.006

Lim, S. N., Cheung, P. C., Ooi, V. E., & Ang, P. O. (2002). Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. Journal of Agricultural and Food Chemistry, 50(13), 3862-3866.

Liu, S. C., Wang, Q., Lienhard, G. E., & Keller, S. R. (1999). Insulin receptor substrate 3 is not essential for growth or glucose homeostasis. Journal of Biological Chemistry, 274, 18093–18099. https://doi.org/10.1074/jbc.274.25.18093

Maheshwari, A., Verma, N., Swaroop, A., Bagchi, M., Preuss, H. G., Tiwari, K., & Bagchi, D. (2017). Efficacy of Furosap™, a novel Trigonella foenum-graecum seed extract, in enhancing testosterone level and improving sperm profile in male volunteers. International Journal of Medical Sciences, 14(1), 58.‏

Maia, A. L., Favaretto, A. L., Antunes-Rodrigues, J., Iazigi, N., & Lamano-Carvalho, T. L. (1990). Spermatogenic and steroidogenic testicular function in hypothyroid pubertal rats. Brazilian Journal of Medical and Biological Research, 23(6-7), 625-628.

Mishra, P., Paital, B., Jena, S., Swain, S. S., Kumar, S., Yadav, M. K., Chainy, G. B. N., & Samanta, L. (2019). Possible activation of NRF2 by Vitamin E/Curcumin against altered thyroid hormone-induced oxidative stress via NFκB/AKT/mTOR/KEAP1 signalling in rat heart. Scientific Reports, 9, 7408–7423.

Petit, P., Sauvaire, Y., Ponsin, G., Manteghetti, M., Fave, A., & Ribes, G. (1993). Effects of a fenugreek seed extract on feeding behaviour in the rat: metabolic-endocrine correlates. Pharmacology Biochemistry and Behavior, 45, 369–374.

Price, K. R., Johnson, I. T., & Fenwick, G. R. (1987). The chemistry and biological significance of saponins in foods and feeding stuffs. Critical Reviews in Food Science and Nutrition, 26, 27–135.

Raghuram, T. C., Sharma, R. D., Sivakumar, B., & Sahay, B. K. (1994). Effect of fenugreek seeds on intravenous glucose disposition in non-insulin dependent diabetic patients. Phytotherapy Research, 8, 83–86.

Rasheed, M. S. A. A., Wankhade, M. V., Saifuddin, M. S. S. K., & Sudarshan, M. A. R. (2015). Physico-chemical properties of Fenugreek (Trigonella foenum-graecum L.) seeds. International Journal of Engineering Research, 4, 68–70.

Sakr, S. A., El-Shenawy, S. M., & Al-Shabka, A. M. (2012). Aqueous fenugreek seed extract ameliorates adriamycin-induced cytotoxicity and testicular alterations in albino rats. Reproductive Sciences, 19(1), 70-80.1- Kaplan E. L. Schwartz, S.I.; Shires, G.T.; Spencer, F.C. Storer, E.H. (1984). Principles of surgery in Thyroid and parathyroid, 4th edn., McGraw-Hill, New York, 1545-1635.

Setty, B. S., Kamboj, V. P., & Khanna, N. M. (1977). Screening of plants for biological activity: Part VII, spermicidal activity of Indian plants. Indian Journal of Experimental Biology, 15, 231.

Shamim, M., Naseem, E., & Khan, N. I. (2016). Hypolipidemic effects of Trigonella foenum-graecum (fenugreek) seed powder administration in rabbits with experimental dietary hyperlipidemia. FUUAST Journal of Biology, 6(1), 33-39.

Sharma, N., & Jacob, D. (2002). Assessment of reversible contraceptive efficacy of methanol extract of Mentha arvensis L. leaves in male albino mice. Journal of Ethnopharmacology, 80, 9-13.

Singh, A., Sarkar, D., & Singh, S. K. (2022). Effect of Trigonella foenum-graecum L. seed extract on the reproductive system of male mice and possible mechanism of its action on spermatogenesis. Andrologia, 54(7), 1643-1659.‏

Singh, M., & Verma, G. N. (2019). Effect of ethanolic extract of Trigonella foenum-graecum L. seeds on reproductive system of male albino rats. Journal of Complementary and Alternative Medical Research, 8(3), 1-8.

Singh, S., Panda, V., Sudhamani, S., & Dande, P. (2020). Protective effect of a polyherbal bioactive fraction in propylthiouracil-induced thyroid toxicity in rats by modulation of the hypothalamic–pituitary–thyroid and hypothalamic–pituitary–adrenal axes. Toxicology Reports, 7, 730-742.‏

Stark, A., & Madar, Z. (1993). The effect of an ethanol extract derived from fenugreek (Trigonella foenum-graecum) on bile acid absorption and cholesterol levels in rats. British Journal of Nutrition, 69, 277–287.

Statistical Packages for the Social Sciences (SPSS). (2010). Statistical software for windows version 19.0. Microsoft. SPSS, Chicago, Illinois, USA. https://stagenew294.weebly.com/spss-19-0-software.html

Swaroop, A., Jaipuriar, A. S., Sushil, K. G., Manashi, B., Pawan, K., Harry, & Debasis, B. (2015). Efficacy of a novel fenugreek seed extract (Trigonella foenum-graecum, Furocyst™) in polycystic ovary syndrome (PCOS). International Journal of Medical Sciences, 12(10), 825-831.

Vats, V., Grover, J. K., & Rathi, S. S. (2002). Evaluation of antihyperglycemic and hypoglycemic effect of Trigonella foenum-graecum Linn, Ocimum sanctum Linn, and Pterocarpus marsupium Linn in normal and alloxanized diabetic rats. Journal of Ethnopharmacology, 79(1), 95–100.

Zia, T., Hasnain, S. N., & Hasan, S. K. (2001). Evaluation of the oral hypoglycemic effect of Trigonella foenum-graecum L (methi) in normal mice. Journal of Ethnopharmacology, 75(2-3), 191–195.

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