IL-6 and TNFα Genetic Mutations and Immunological Response in Salmonellosis
Zina Murshd Kadim 1, Nahla Ghazi Mohammed Al Loza 2,4*, Teba Habeeb Sayfe 3
Journal of Angiotherapy 8(5) 1-7 https://doi.org/10.25163/angiotherapy.859668
Submitted: 17 March 2024 Revised: 06 May 2024 Published: 11 May 2024
Abstract
Background: Understanding the genetic and immunological aspects of Salmonella infection is essential for effective management and prevention strategies. This study aimed to investigate the prevalence of Salmonella IgM and IgG antibodies, as well as the concentrations of interleukin-6 (IL-6) and tumor necrosis factor alpha (TNFα), in patients with suspected Salmonella infection compared to healthy controls. Additionally, genetic mutations in the IL-6 and IFNα genes were explored. Methods: Between March and November 2023, blood samples were collected from 60 patients visiting Baghdad Teaching Hospital and 30 healthy individuals serving as the control group. ELISA assays were performed to measure Salmonella IgM and IgG levels, while IL-6 and TNFα concentrations were determined. Genetic mutations in the IL-6 (rs1800799) and IFNα (rs2430560) genes were analyzed using molecular techniques. Results: The mean levels of Salmonella IgM and IgG did not significantly differ between males and females (p = 0.21 and p = 0.58, respectively). Similarly, there were no significant gender differences in IL-6 and TNFα concentrations (p = 0.55 and p = 0.55, respectively). Mutations were observed in the IL-6 gene (rs1800799), resulting in variations from the wild AA variant to AG, AG, GG, GG, GG, AG, AG, GG, AG, and GG. Additionally, mutations were detected in the IFNα gene (rs2430560), with the wild TT variant changing to TA, TA, TA, AA, AA, TA, TA, AA, TT, and AA. Conclusion: This study provides insights into the immunological and genetic aspects of Salmonella infection. The lack of significant gender differences in antibody levels and cytokine concentrations suggests a similar immune response between males and females. The identification of genetic mutations in the IL-6 and IFNα genes highlights the genetic variability associated with Salmonella infection and warrants further investigation into their clinical implications.
Keywords: Salmonellosis, genetic mutations, immunological response, IL-6, TNFα
References
Abbott, S. L., Janda, J. M., & Farmer, J. J. (2011). Vibrio and related organisms. In J. Versalovic, K. C. Carroll, J. H. Jorgensen, G. Funke, M. L. Landry, & D. W. Warnock (Eds.), Manual of clinical microbiology (10th ed., Vol. 1, pp. 666–676). ASM Press.
Allos, B. M., & Blaser, M. J. (2010). Campylobacter jejuni and related species. In G. L. Mandell, J. E. Bennett, & R. Dolin (Eds.), Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases (7th ed.). Elsevier.
Blaser, M. J. (2020). Helicobacter pylori and other gastric Helicobacter species. In G. L. Mandell, J. E. Bennett, & R. Dolin (Eds.), Mandell, Douglas and Bennett’s Principles and Practice of Infectious Diseases (7th ed.). Elsevier.
Calenge, F., Kaiser, P., & Vignal, A. (2010). Genetic control of resistance to salmonellosis and to Salmonella carrier-state in fowl: A review. Genetics Selection Evolution, 42, Article 11. https://doi.org/10.1186/1297-9686-42-11
Chiwan, A. H. R., & Shnawa, I. M. S. (2023). Diagnostic validity of urinary mucosal anti-Salmonella typhi OMP antibodies as compared to systemic responses in typhoid patients. Journal of Population Therapeutics and Clinical Pharmacology, 30(12). https://doi.org/10.47750/jptcp.2023.30.12.012
Chiwan, A. H., Shnawa, I. M., & Abd, F. G. (2023). Genome DNA sequencing, phylogenetic dendrogram and outer membrane protein separation studies of local Najaf S. typhi isolates/Iraq. HIV Nursing, 23(1), 1125-1128.
Dahora, L. C., Jin, C., Spreng, R. L., Feely, F., Mathura, R., Seaton, K. E., Zhang, L., Hill, J., Jones, E., Alam, M., Dennison, S. M., Pollard, A. J., & Tomaras, G. D. (2019). IgA and IgG1 specific to Vi polysaccharide of Salmonella Typhi correlate with protection status in a typhoid fever controlled human infection model. Frontiers in Immunology, 10, 2582. https://doi.org/10.3389/fimmu.2019.02582
Fitzgerald, C., & Nachamkin, I. (2011). Campylobacter and Arcobacter. In J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, & D. W. Warnock (Eds.), Manual of clinical microbiology (10th ed., pp. 885–899). American Society for Microbiology.
Guo, Y., Gu, D., Huang, T., Cao, L., Zhu, X., Zhou, Y., Wang, K., Kang, X., Meng, C., Jiao, X., & Pan, Z. (2020). Essential role of Salmonella Enteritidis DNA adenine methylase in modulating inflammasome activation. BMC Microbiology, 20, Article 226. https://doi.org/10.1186/s12866-020-01873-7
Horneman, A. J., & Ali, A. (2011). Aeromonas. In J. Versalovic, K. C. Carroll, G. Funke, J. H. Jorgensen, M. L. Landry, & D. W. Warnock (Eds.), Manual of clinical microbiology (10th ed., pp. 658–665). ASM Press.
Huang, F.-C. (2009). Upregulation of Salmonella-induced IL-6 production in Caco-2 cells by PJ-34, PARP-1 inhibitor: Involvement of PI3K, p38 MAPK, ERK, JNK, and NF-κB. Mediators of Inflammation, 2009, Article 103890. https://doi.org/10.1155/2009/103890
Jin, C., Gibani, M. M., Moore, M., Juel, H. B., Jones, E., Meiring, J., Harris, V., Gardner, J., Kerridge, S. A., Hill, J., Thomaides-Brears, H., Blohmke, C. J., Yu, L. M., Angus, B., & Pollard, A. J. (2017). Efficacy and immunogenicity of a Vi-tetanus toxoid conjugate vaccine in the prevention of typhoid fever using a controlled human infection model of Salmonella Typhi: A randomised controlled, phase 2b trial. The Lancet, 390, 2472-2480. https://doi.org/10.1016/S0140-6736(17)32149-9
Jones, E., Jin, C., Stockdale, L., Dold, C., Pollard, A., & Hill, J. (2021). A Salmonella Typhi controlled human infection study for assessing correlation between bactericidal antibodies and protection against infection induced by typhoid vaccination. Microorganisms, 9(7), 1394. https://doi.org/10.3390/microorganisms9071394
Mariam Ayad Abd, Sawsan Q. T. Al-Quhli, Mahir Ali Jassim, (2024). Salmonella Biofilms And Antibiotic Resistance Determination From Different Patient Types, Journal of Angiotherapy, 8(2), 1-11, 9502
Mohan, V. K., Varanasi, V., Singh, A., Pasetti, M. F., Levine, M. M., Venkatesan, R., & Ella, K. M. (2015). Safety and immunogenicity of a Vi polysaccharide-tetanus toxoid conjugate vaccine (Typbar-TCV) in healthy infants, children, and adults in typhoid endemic areas: A multicenter, 2-cohort, open-label, double-blind, randomized, controlled phase 3 study. Clinical Infectious Diseases, 61, 393-402. https://doi.org/10.1093/cid/civ324
Najib, M. A., Mustaffa, K. M. F., Ong, E. B. B., Selvam, K., Khalid, M. F., Awang, M. S., Zambry, N. S., Manaf, A. A., Bustami, Y., Hamzah, H. H., Ismail, A., & Aziah, I. (2021). Performance of immunodiagnostic tests for typhoid fever: A systematic review and meta-analysis. Pathogens, 10(11), 1394. https://doi.org/10.3390/pathogens10111394
Pham, T. H. M., Brewer, S. M., Thurston, T. L. M., Massis, L. M., Honeycutt, J., Lugo, K., Jacobson, A. R., Vilches-Moure, J. G., Hamblin, M., Helaine, S., & Monack, D. M. (2020). Salmonella-driven polarization of granuloma macrophages antagonizes TNF-mediated pathogen restriction during persistent infection. Cell Host & Microbe, 27(1), 54-67.e5. https://doi.org/10.1016/j.chom.2019.12.002
Qadri, F., Khanam, F., Liu, X., Theiss-Nyland, K., Biswas, P. K., Bhuiyan, A. I., Ahmmed, F., Colin-Jones, R., Smith, N., Tonks, S., Voysey, M., Mujadidi, Y. F., Mazur, O., Rajib, N. H., Hossen, I., Ahmed, S. U., Khan, A., Rahman, N., Babu, G., ... Pollard, A. J. (2021). Protection by vaccination of children against typhoid fever with a Vi-tetanus toxoid conjugate vaccine in urban Bangladesh: A cluster-randomised trial. The Lancet, 398(10301), 675-684. https://doi.org/10.1016/S0140-6736(21)01124-7
Seas, C., DuPont, H. L., Valdez, L. M., & Gotuzzo, E. (1996). Practical guidelines for the treatment of cholera. Drugs, 51(6), 966-973. https://doi.org/10.2165/00003495-199651060-00006
Shakya, M., Colin-Jones, R., Theiss-Nyland, K., Voysey, M., Pant, D., Smith, N., Liu, X., Tonks, S., Mazur, O., Farooq, Y. G., Hill, J., Adhikari, A., Dongol, S., Karkey, A., Bajrach
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