Therapeutic Effects of Esomeprazole, Curcumin, Chitosan, and Curcumin-Chitosan Mixture on Ethanol-Induced Gastric Ulcer in Female Rats
Jenan Mahdi Jawad Al-kawaz 1*, Fakhir Magtoof Al-Zubaidy 2**, Hussein Jassim Obaid Al -Harbi 1***
Journal of Angiotherapy 8(1) 1-12 https://doi.org/10.25163/angiotherapy.819439
Submitted: 19 December 2023 Revised: 18 January 2024 Published: 21 January 2024
Abstract
Introduction: Gastric ulcer (GU) is a prevalent health issue linked to alcohol consumption, smoking, and physiological stress. This study aims to assess the therapeutic effects of esomeprazole (40 mg), curcumin (40 mg/kg), chitosan (150 mg/kg), and a mixture of curcumin (40 mg/kg) and chitosan (150 mg/kg) on ethanol-induced gastric ulcers in female rats. Method: The study involved 30 female rats with an average weight ranging from 179.1 to 180.3 gm. Rats were divided into two control groups and four treated groups (esomeprazole, curcumin, chitosan, and mixture), each containing 5 rats. Gastric ulcers were induced by orally administering 2 ml of absolute ethanol to All groups, except the negative control, after a 19-hour fasting period. Therapeutic effects were evaluated by assessing gastric juice volume and pH, ulcer index, curative index, and through morphological and histological examination of the stomach. Results: The study revealed a significant decrease (p≤0.05) in body weight percentage change in the curcumin-treated group compared to the positive control. Esomeprazole, chitosan, and the mixture showed mixture showed no significant change (p>0.05). Additionally, esomeprazole, curcumin, chitosan, and the mixture demonstrated a significant decrease (p≤0.05) in ulcer index and gastric juice volume, with a significant increase (p≤0.05) in pH compared to the positive control. Conclusion: In conclusion, esomeprazole, chitosan, and the mixture demonstrated notable protective effects and therapeutic effects by reducing ulcer index and gastric juice volume in ethanol-induced gastric ulcers in female rats.
Keywords: Gastric ulcer, stomach, curcumin, Esomeprazole, Chitosan, Female rats
References
Adinortey, M. B., Ansah, C.,Galyuon, I, et al. (2013). In vivo models used for evaluation of potential antigastrduodenal ulcer model agents. Ulcers, 2013:1-12.
Aggarwal BB, Sundaram C, Malani N and Ichikawa H, 2007. Curcuminn: The Indian solid gold. Adv Exp Med Biol. 95:1-75.
Aguiar, A.S., Da-Silva, V.A., and Boaventura, G.T. (2004). Can calories from ethanol contribute to body weight preservation by malnourished rats? Braz J Med Biol Res (37): 841-846.
Ahmed S, Ahmad M, and Ikram S, 2014. Chitosan a Natural Antimicrobial Agent-A Review. J. Applicable Chem. 3(2): 493-503.
Aistrup G. L., Marszalec, W., Narahashi, T.(1999). Ethanol modulation of nicotinic acetylcholine receptor currents in cultured cortical neurons. Molecular Pharmacology,(55)1:39-49. https://doi.org/10.1124/mol.55.1.39
Al-Judaibi A, Chande N, Dresser GK, Sultan N and Gregor JC, 2010. Gastric Acid-Related Diseases: Focus on Esomeprazole. Clinical Medicine Insights: Therapeutics. 2: 439-452.
Ammon H and Wahl MA,1991.Pharmacology of Curcuma longa. Planta Med.57:1-7.
Araugo, E.R.D., Guerra, G.C.B., Andrade, A.W.L., Fernandes, J.M., Silva, V.C.D., Tavares, E.D.A., Araujo, A.A.D., Junior, R.F.D.A., and Zucolotto, S.M. (2021). Gastric ulcer healing property of bryophyllum pinnatum leaf extract in chronic model in vivo and gastroprotective activity of its major flavonoid. frontiers in Pharmacology, 12(744192):1-21
Bancroft JD, Layton C and Suvarna SK, 2013. Bancrofts theory and practice of histological technique. Seventh edition.Livingstone.Elsevier Limited.
Bhattacharyya, A., Chattopadhyay, R.,Mitra, S., and Crowe, S.E. (2023). Oxidative stress: An essential factors in the pathogenesis of gastrointestinal mucosal diseases. Physiol Rev.94(14):329-354.
Castro, C.L., Aguiar-Nemer, A.S., Castro-Faria-Neto, H.C., Barros, F.R., Rocha, E.M.S. and Slva-Fonseca, V.A.(2013). Effect of chronic ethanol consumption in females rats subjected to experimental sepsis. Braz J Med Biol Res, 46(12): 1033-1039. http://dx.doi.org/10.1590/1414-431x20133189
Devlieghere F, Vermeulen, A and Debevere J, 2004. Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol. 21: 703–714.
Dokmeci D, Akpolat M, Aydogdu N, Doganay L. and Turan FN, 2005. L-carnitine inhibits ethanol-induced gastric mucosal injury in rats. Pharmacology reports.57:481-488.
French, T.A. & Weiner, N. (1991). Serotonin-ergic involvement in ethanol-induced alterations of thermoregulation in longsleep and short-sleep mice. Journal of Pharmacology and Experimental Therapeutics, (259): 833-840.
Garcia, J.A.D., Souza, A.L.T., Cruz, L.H.C., Marques, P.P., Camilli, J.A., Nakagaki, W.R., Esteves, A.,Rossi-Junior, W.C.,Fernandes, G.J.M.,Guerra, F.D.,Soares, E.A. (2015). Effects of ethanol consumption and alcohol detoxification on the biomechanics and morphology the bone in rat femurs. Braz. J. Biol.,75(4):983-988. http://dx.doi.org/10.1590/1519-6984.04814
Hajrezaie, M., Salehen, N., Karimian,H., et al. (2015). Biochanin a gastroprotective effects in ethanol-induced gastric mucosal ulceration in rats. PloS One,10:e0121529.
Ibrahim, H. A. E. , Metwaly, E. K., Galal, A. A. A., and Sherif, S. A. (2019). Potential curative of curcumin on gastric ulcer induced by piroxicam in male albino rats. Zag Vert J,47(4):378-387. doi:10.21608/zvjz.2019.14389.1056
Kalant, H. and Lê, A.D. (1984). Effects of ethanol on thermoregulation. Pharmacologyand Therapeutics, (23): 313-364.
Karasov WH, Pinshow B, Starck JM and Afik D, 2004. Anatomical and histological changes in the alimentary tract of migrating blackcaps (Sylvia atricapilla):a comparison among fed, fasted,food-restricted, and refed birds. Physiological and Biochemical Zoology.77:149-160.
Koboziev, I., Scoggin, S., Gong, X., Mirzaei, P.,Zabet-Moghaddam, M., Yosofvand, M., Moussa, H., Jones-HAll,Y.and Moustaid-Moussa, N.(2020). Effects of curcumin in a mouse model of very high fat diet- induced obesity. Biomolecules,(10)1368:1-16.
Kuadkaew S, Ungphaiboon S, Phdoongsombut, N, Kaewsuwan, S and Mahattanadul, S, 2021. Efficacy of a chitosan-curcumin mixture in treating indomethacin-induced acute gastric ulcer in rats. Current pharmaceutical biotechnology. 22:1919-1931.
Kumar V, Cotran R, Robbins S, editors. (2003). Robbins basic pathology. 7th ed. Philadephlia: W.B. Saunders.
Lind T, Rydberg L, Kyleback A, Jonsson A, Andersson T and Hasselgren G, 2000. Esomeprazole provides improved acid control vs. omeprazole In patients with symptoms of gastro-oesophageal reflux disease. Aliment Pharmacol Ther.14:861–7.
Ma N, Sasaki T, Sakata-Haga H, Ohta K, Gao M, awanishi S and Fukui Y, 2017. Protective effect of taurine against nitrosative stress in the stomach of rat with water immersion resistant stress. Adv. Exp. Med. Biol.643:273-283.
Miner PJR , Katz PO, Chen Yand Sostek M, 2003. Gastric acid control with esomeprazole, lansoprazole, omeprazole, pantoprazole, and rabeprazole: a five-way crossover study. Am J Gastroenterol. 98:2616–20.
Mizinga, K.M., Stino, F.K.R., Samaan, S.S., Soliman, K.F.A. and Kolta, M.G. (1995). Hypothermic effect of ethanol in mice selected for differential sleep-time responses to pentobarbital. Pharmacology, Biochemistry and Behavior, (51): 525-528.
Mohammad R, Shahid M , Jardan AB and Ansari MA ,2021. Gastroprotective effect of sinapic acid on thanol-induced gastric ulcers in rats: Involvement of Nrf2/HO-1 and NF-kB signaling and antiaptotic role. Frontiers in Pharmacology.12:1-15.
Morato, G.S., Souza, M.L.O., Pires, M.L.N. and Masur, J. (1986). Hypoglycemia and hypothermia induced by ethanol: antagonism by indomethacin. Pharmacology, Biochemistry and Behavior, (25): 739-742.
Morikawa, H. and Morrisett, R.A.(2010). Ethanol action on dopaminergic neurons in the ventral tegmental area: interaction with intrinsic ion channels and neurotransmitter inputs. Int Rev Neurobiol,(91):235-288 doi:10.1016/s0074-7742(10)91008-8
Noorafshan A. and Ashkani-Esfahani S, 2013. A Review of Therapeutic Effects of Curcumin. Current Pharmaceutical Design. 19: 2032-2046.
Obasi E, Iheanacho K, Nwachukwu Nc and Chikezie, PC, 2019. Evaluation of body weight, serum glucose level and oxidative stress parameters of diabetic rats administration phenolic aqueous leaf extract of Vitex doniana . Research and Therapy.6:3359-3367.
Palmese F., Bonavita, M. E., Pompili1, E., Migliano, M.T., Reggidori1,N., Stefano, C. D., Grieco, M., Colazzo, S., Tufoni1, M., Baldassarre, M, Caraceni, P., Foschi, F.G., Giostra, F., Farina G., Toro, R. D., Giorgio Bedogni, G., Domenicali1, M. (2023). Mortality and its association with chronic alcohol-related diseasesin patients admitted to the emergency department for acute alcoholicintoxication: retrospective cohort study. Internal and Emergency Medicine , 18:257–263. https://doi.org/10.1007/s11739-022-03114-6
Pandeya NK and Wives T, 2005. Modern Miracles —Turmeric as Traditional Medicine in India. Trees for Life J. 1:3.
Piano, M.R., Artwohl, J., Kim,S.D., Gass, G. (2001). The effects of a liguid ethanol diet on nutritional status and fluid balance in the rat. Alcohol & Alcoholism, 36(4):298-303.
Rahman,Z., Dwivedi,D.K. and Jena, G.B.(2020). Ethanol-induced gastric ulcer in rats and intervention of tert-butylhydroquinone: Involvement of Nrf2/HO-I signaling pathway. Human and Experimental Toxicology,39(4):547-562.
Rehman, M.H., Tahir, M., Lone, K.P., Sami, W.(2011). Ethanol Induced Hepatotoxicity in Albino Rats. Journal of the college of Physicians and Surgeons Pakistan, 21(10):642-643.
Ronis M. J., Hakkak R., Korourian S., Albano E, Yoon S, IngelmanSundberg M, et al. (2004). Alcoholic liver disease in rat fed ethanol aspart of oral or intragastric low-carbohydrate liquid diets. Exp Biol Med, 229:351-60.
Saravanan N, Nalini N. (2007) Antioxidant effect of Hemidesmus indicuson ethanol induced hepatotoxicity in rats. J Med Food, 10:675-82.
Scott LJ, Dunn CJ, MAllarkey G and Sharpe M. 2002. Esomeprazole: a review of its use in the management of acid-related disorders. Drugs. 62:1503–38.
Shefner, S.A. and Tabakoff, B. (1985). Basalfiring rate of rat locus coeruleus neurons affects sensitivity to ethanol. Alcohol, 2: 239-243.
Shimizu,N., Watanabe,T., Arakawa,T., Fujiwara,Y., Higuchi,K., Kuroki,T.(2000). Pentoxifylline Accelerates Gastric UlcerHealing in Rats: Roles of TumorNecrosis Factor Alpha and Neutrophils during theEarly Phase of Ulcer Healing. Digestion,(61):157–164.
Shin JW, Soel IC and Son CG, 2010. Interpretation of Animal Dose and Human Equivalent Dose for Drug Development. Journal of Korean Oriental Medicine.31:1-7.
Somensi, L. P., Costa, P., Boeing, T. , Mariano, N.,L.,B., Grego´ rio, E.,D., Silva, A.,T.,M.,E., Longo, B., Locatelli, C., de Souza, P., Magalhães, C., G., Duarte, L.P. and Silva, L., M., D.(2022). Lupeol stearate accelerates healing and prevents recurrence of gastric ulcer in rodents, Volume 2022, Article ID 6134128, 10 pages https://doi.org/10.1155/2022/6134128
Tabakoff, B., Hoffman, P.L. and Moses, F (1977). Neurochemical correlates of ethanol withdrawal: alterations in serotonergic function. Journal of Pharmacy and Pharmacology,(29): 471-476.
Tabakoff, B. and Hoffman, P.L. (1991). Neurochemical effects of alcohol. In: Frances RJ & Miller SI (Editors), Clinical Textbook of Addictive Disorders. Guilford Press, New York, 501-525.
Tabakoff B., Hellevuo, K. and Hoffman, P.L. (1996). Alcohol. In: Schuster, C.R., Gust, S.W. and Kuhar, M.J. (Editors), Handbook of Experimental Pharmacology. Vol. 118. Pharmacological Aspects of Drug Dependence. Springer-Verlag, Berlin, 373-458.
Thakur R., Puri HS, Husain A,1989. Major medicinal plants of India Medicinal plants; Materia medica, Vegetable; Traditional medicine; India. Lucknow: Central Institute of Medicinal and Aromatic Plants. P.585.
Tilak J, Banerjee M., Mohan H and Devasagayam TPA 2004. Antioxidant availability of turmeric in relation to its medicinal and culinary uses. Phytotherapy Research.. 18: 798-804.
Wang, X., Lemos, J.R., Dayanithi, G., Nordmann, J.J. and Treistman, S.N. (1991). Ethanol reduces vasopressin release byinhibiting calcium currents in nerve terminals. Brain Research, (551): 338-341.
Yu, H. P., Chaudry, I. H. (2009). The role of estrogen and receptor agonists in maintaining organ function after trauma-hemorrhage. Shock. (31): 227 – 237. doi:10.1097/SHK.0bo13e31818347e7.
View Dimensions
View Altmetric
Save
Citation
View
Share