Anti-Cancer Study of Carvacrol in Hypoxic-Induced Colorectal Cancer Cell

Aumaima Tariq Abed ^1,2* ,  Ahmed MH AlMudhafar ¹ , and Najah R Hadi ¹

Effect of carvacrol on proliferation and migration of hypoxic SW480 cancer cells

Abstract

Introduction: Colorectal cancer presents a complex global health challenge, characterized by complications arising from metastasis development due to rapid proliferation, adaptation to hypoxia, and angiogenesis. Carvacrol, a natural monoterpenoid phenol derived from various plants, has interest for its diverse pharmacological properties, particularly its antitumor effects. Methods: We aimed to assess the inhibitory effect of carvacrol on cell migration and proliferation in the hypoxic colorectal cancer cell line (SW480). Chemical induction of hypoxia using cobalt chloride and serially diluted concentrations of carvacrol (400, 200, 100, 50, 25, and 12.5 µg/ml) were employed to evaluate the cytotoxic effect via the MTT assay. Smaller concentrations (low IC50) were used to examine the impact of carvacrol on SW480 cell migration through a cell migration assay (50, 25, 12.5, and 6.25 µg/ml). Results: The results demonstrated a significant, dose-dependent reduction in both cell proliferation and migration with carvacrol doses.  Conclusion: The findings suggest that carvacrol could be useful as an adjuvant therapy and a promising addition for patients with highly metastatic colorectal cancer.

Keywords: Carvacrol, hypoxia, colorectal, SW480, proliferation, migration.

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