A Rapid Diagnosis Development of Canine Enteric Coronavirus Inflammation
Mohammed Ali Hussein 1,2*, Mawlood Abbas Ali Al-Graibawi 1
Journal of Angiotherapy 8(4) 1-8 https://doi.org/10.25163/angiotherapy.849521
Submitted: 28 January 2024 Revised: 04 April 2024 Published: 07 April 2024
Abstract
Background: Canine Enteric Coronavirus (CECoV) is a significant viral pathogen causing gastrointestinal disturbances in dogs worldwide. However, data regarding CECoV in Iraq remains scarce. This study aimed to investigate CECoV IgG antibodies using ELISA and detect CECoV antigens in feces using lateral flow immunochromatography test. Methods: A total of 211 blood and fecal samples were collected from 161 dogs with diarrhea and 50 apparently healthy dogs (control group) in Baghdad, Iraq. Clinical signs were recorded, and samples were analyzed for CECoV IgG antibodies and antigens. Hematological parameters were also assessed. Results and Discussion: Clinical examination revealed gastrointestinal disturbances in 76.3% of dogs, with diverse clinical signs observed. ELISA results showed 21.8% seropositivity for CECoV IgG antibodies, with none detected in apparently healthy dogs. Lateral flow immunochromatography identified CECoV antigens in 7.58% of fecal swabs. Hematological changes included leukopenia, lymphopenia, and increased PCV and MCV values. These findings align with previous studies and suggest the ongoing prevalence and clinical significance of CECoV infection in Iraqi dogs. Conclusion: The combined use of ELISA and lateral flow immunochromatography tests can facilitate the diagnosis of CECoV infection in canine practice. This study provides valuable insights into the epidemiology and clinical impact of CECoV in Iraq, contributing to the global understanding of this viral pathogen.
Keywords: Dog, Canine enteric coronavirus (CECoV), ELISA, immunochromatography, Zoonotic disease, Public health
References
Alfano, F., Fusco, G., Mari, V., et al., 2020. Circulation of pantropic canine coronavirus in autochthonous and imported dogs, Italy. Transbound. Emerg. Dis. https://doi.org/ 10.1111/tbed.13542.
AL-Mutar, H. A. H. (2020). The evolution of Genetic Molecular Map and phylogenetic tree of Coronavirus (COVID-19). The Iraqi Journal of Veterinary Medicine, 44(2), 56–70.
Avci O, Levent O, Yapici Orhan, Hasircioglu S, Simsek A. (2016). Canine coronavirus infection in dogs in Türkiye: Virological and serological evidence. Indian Journal of Animal Research.
Avci, O., Yavru, S., Kale, M. and Dik, I.: (2015) Determination of presence of canine coronavirus in shelter dogs. Eurasian. J. Vet. Sci. 31: 184-187.
Awad, R. A., S. Ali Hassan, A. G. Attallah & W. K. B. Khalil, 2020. Epidemiology and molecular diagnosis of canine coronavirus in Egypt: evaluation of different tests used for its diagnosis. Bulg. J. Vet. Med., 23, No 4, 467-477.
Awadallah MAI, Salem LMA.(2015) Zoonotic enteric parasites transmitted from dogs in Egypt with special concern to Toxocara canisinfection.Vet World., 8(8):946-957.
Badawi N.M, Yousif A.A.(2020) Babesia canis spp. in dogs in Baghdad Province, Iraq: First molecular identification and clinical and epidemiological study. Vet World. 13(3):579-585.
Buller H, Adam K, Bard A, Bruce A, (Ray) Chan KW, Hinchliffe S, Morgans L, Rees G, Reyher KK. (2020). Veterinary Diagnostic Practice and the Use of Rapid Tests in Antimicrobial Stewardship on UK Livestock Farms. Frontiers in Veterinary Science, 7: 765.
Castro TX, Cubel Garcia Rde C, Gonçalves LP, Costa EM, Marcello GC, Labarthe NV, Mendes-de-Almeida F.(2013) Clinical, hematological, and biochemical findings in puppies with coronavirus and parvovirus enteritis. Can Vet J. Sep;54(9):885-8.
Decaro N, Buonavoglia C. An update on canine coronaviruses: viral evolution and pathobiology. Vet Microbiol. (2008) 132:221– 34.
Decaro N, Campolo M, Lorusso A, Desario C, Mari V, Colaianni ML, Elia G, Martella V, Buonavoglia C.(2008). Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia. Vet Microbiol. Apr 30;128(3-4):253-60.
Decaro N, Cordonnier N, Demeter Z (2013). European surveillance for pantropic canine coronavirus. J Clin Microbiol. 51:83–88.
Dema A., Tallapally M R., Ganji V K., Buddala B., Kodi H., Ramidi A., Yella N R. and Putty K. (2022). A Comprehensive Molecular Survey of Viral Pathogens Associated with Canine Gastroenteritis, 02, Preprint (Version 1) available at Research Square.
DiGangi BA, Dingman PA, Grijalva CJ (2019) Prevalence and risk factors for the presence of serum antibodies against canine distemper, canine parvovirus, and canine adenovirus in communities in mainland Ecuador. Vet Immunol Immunopathol.
Elia G, Fiermonte G, Pratelli A, Martella V, Camero M, Cirone F and Buonavoglia C. (2003). Recombinant M protein-based ELISA test for detection of antibodies to canine coronavirus. Journal of Virological Methods 109.139_/142
Enany M, Wahdan A, El-Metwaly M et al (2021) Bacterial Causes of Hemorrhagic Gastroenteritis in Dogs and Cats with Detection of Some Virulence and β-lactamase Resistance Genes in Escherichia coli and Salmonella by Multiplex PCR. Suez Canal Vet Med Journal SCVMJ 0:0–0.
Evermann, J.F., Abbott, J.R., Han, S (2005). Canine coronavirus-associated puppy mortality without evidence of concurrent canine parvovirus infection. J. Vet. Diagn. Invest. 17 (6), 610–614.
Faraj, A. A., Shaimaa, A. M., Shahla, R. F. (2018). Prevalence of Cryptosporidium spp. in feces of 160 domestic dogs in Baghdad, Iraq. Online Journal of Veterinary Research. Volume 22 (7):563-567.
Gerardi F, Santaniello A, Del Prete L et al (2018) Parasitic infections in dogs involved in animal-assisted interventions. Ital J Anim Sci 17:269–272.
Ghazzi, J. J., Fadhil , H. Y., & Aufi , I. M. (2023). Impact of SARS-COV-2 Variants on the Infection Severity among Iraqi Patients. Iraqi Journal of Veterinary Science, 64(7), 3263–3272.
Giannitti, F.; Diab, S.; Mete, A.; Stanton, J.B.; Fielding, L.; Crossley, B.; Sverlow, K.; Fish, S.; Mapes, S.; Scott, L.; et al. Necrotizing Enteritis and Hyperammonemic Encephalopathy Associated with Equine Coronavirus Infection in Equids. Vet. Pathol. 2015, 52, 1148–1156.
Gizzi ABDR, Oliveira ST, Leutenegger CM et al (2014) Presence of infectious agents and co-infections in diarrheic dogs determined with a real-time polymerase chain reaction-based panel. BMC Vet Res 10:23.
Gnirs, K.; Quinton, J.F.; Dally, C.; Nicolier, A.; Ruel, Y. Cerebral pyogranuloma associated with systemic coronavirus infection in a ferret. J. Small Anim. Pract. 2016, 57, 36–39.
Gur S, Gencay A Dogan N. (2008). A Serologic Investigation for Canine Corona Virus Infection in Individually Reared Dogs in Central Anatolia. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 5(2): 67-71.
Hana W. Jun Chen, Jegathambigai Rameshwar Naidu, Mohamed Najimudeen. (2024). A Post-Pandemic Insight on The Covid-19 and Pregnancy in Malaysia, Journal of Angiotherapy, 8(2), 1-3, 9454
Hasan M. S., Yousif A.A. and Alwan M. J. (2016). Detection of Virulent Genes in E. coli O157:H7 Isolated from Puppies and Adult Dogs by Polymerase Chain Reaction. Research Journal for Veterinary Practitioners. 4 (1) 1-6.
Jassim, H. S. (2021). Efficacy of Immune System Challenges with Tiny Enemy COVID-19. The Iraqi Journal of Veterinary Medicine, 44(1), 75–79.
Jayanthi E, C. Elayaraja, Priyadharshini S. (2024). Navigating COVID-19 Treatment: CT Scans, Steroids, and Medical Practice Challenges in India, Journal of Angiotherapy, 8(2), 1-4, 9459
Kaneshima, T., Hohdatsu, T., Satoh, K., Takano, T., Motokawa, K. and Koyama, H. (2006). The prevalence of a group 2 coronavirus in dogs in Japan. J. Vet. Med. Sci. 68: 21-25..
Ma, G.G., Lu, C.P (2005). Two genotypes of Canine coronavirus simultaneously detected in the fecal samples of healthy foxes and raccoon dogs. Wei Sheng Wu Xue Bao 45 (2), 305–308.
Mahmood, Z. S., Fadhil, H. Y., and Ad′hiah, A. H (2021). Estimation of Hematological Parameters of Disease Severity in Iraqi Patients with COVID-19. Iraqi Journal of Science, 62(10), 3487–3496.
Mansour, K. A., Hasso, S. A. (2022). Molecular Detection of Canine Distemper Virus in Dogs in Baghdad Province, Iraq. The Iraqi Journal of Veterinary Medicine, 45(2), 46–50.
Marinaro M, Mari V, Bellacicco AL, Tarsitano E, Elia G, Losurdo M, Rezza G, Buonavoglia C, Decaro N (2010). Prolonged depletion of circulating CD4+ T lymphocytes and acute monocytosis after pantropic canine coronavirus infection in dogs. Virus Res. 152(1-2):73-8.
Molnar, B., Duchamp, C., M¨ostl, K., Diehl, P.A., Betschart, B., 2014. Comparative survey of canine parvovirus, canine distemper virus and canine enteric coronavirus infection in free-ranging wolves of central Italy and south-eastern France. Eur. J. Wildl. Res 60 (4), 613–624.
Monge-Maillo B, López-Vélez R, NormanFF, Ferrere-González F, Martínez-Pérez Á, Pérez-Molina JA. Screening of imported infectious diseases among asymptomatic Sub-Saharan African and Latin American immigrants: a public health challenge. Am J Trop Med Hyg. 2015; 92 (4):848-856.
Moniruddin Chowdhury, Syeda Humayra, Taha Sulayman, Keichiro Mihara et al., (2023). Use of Neutralizing Monoclonal Antibodies and Its Outcome Measures in COVID-19 Patients, Journal of Angiotherapy, 7(1), 1-7.
Mosallanejad B, Ghorbanpoor N M, Avizeh R, Nikoosiar J M. 2008. Antigenic detection of Canine Coronavirus in diarrheic dogs in Ahvaz. Int.J.Vet.Res. 2,1:81-85.
Naylor, M.J., Monckton, R.P., Lehrbach, P.R., Deane, E.M., 2001b. Canine coronavirus in Australian dogs. Aust. Vet. J. 79, 116–119.
Owain M S., and Yousif A. A (2018). Effect of lead with or without thiamine and calcium EDTA on hematology in young dogs. Online Journal of Veterinary Research. Volume 22 (6):434-443.
Parkhe P and Verma S (2021) Evolution, Interspecies Transmission, and Zoonotic Significance of Animal Coronaviruses. Front. Vet. Sci. 8:719834.
Pesavento PA, Murphy BG (2014) Common and emerging infectious diseases in the animal shelter. Vet Pathol 51:478–491.
Pratelli A, Tinelli A, Decaro N, Martella V, Camero M, Tempesta M, Martini M, Carmichael LE, Buonavoglia C. Safety and efficacy of a modified-live canine coronavirus vaccine in dogs. Vet Microbiol. 2004 Mar 26;99(1):43-9.
Pratelli, A., 2006. Genetic evolution of canine coronavirus and recent advances in prophylaxis. The Veterinary Research 37, 191–200.
Pratelli, A., Elia, G., Martella, V., Palmieri, A., Cirone, F., Tinelli, A., Corrente, M. and Buonavoglia, C. 2002. Prevalence of canine coronavirus antibodies by an enzyme-linked immunosorbent assay in dogs in the south of Italy. J. Virol. Methods 102: 67–71.
Pugazhandhi Bakthavatchalam, Fouad Saleih R.AL-Suede, Sudarshan S., (2022), A Systematic Review on Temporal Lobe Epilepsy Patients with Corona Virus Infection, Journal of Angiotherapy, 6(2).
Radford AD, Singleton DA, Jewell C et al (2021) Outbreak of severe vomiting in dogs associated with a canine enteric coronavirus, United Kingdom. Emerg Infect Dis 27:517.
Rosa, G.M., Santos, N., Grøndahl-Rosado, R., et al., 2020. Unveiling patterns of viral pathogen infection in free-ranging carnivores of northern Portugal using a complementary methodological approach. Comp. Immunol. Microbiol Infect. Dis. 69, 101432.
Rowland H, Holding E, Falces PM, Wissink-Argilaga N, Stidworthy MF, Denk D, Weir W, Krumrie S, Dunbar D, Hopper JS. 2021. Canine coronavirus subtype 2a associated with outbreaks of fatal diarrhoea in bush dog (Speothos venaticus) groups, Fatale Diarrhoe bei Waldhunden (Speothos venaticus) durch Canines Coronavirus Subtyp 2a. Schweizer Archiv fur Tierheilkunde, 164(10): 661–671.
Salah, H. A.; Fadhil, H. Y.; Alhamdani, F. G. (2020) “multiplex sybr green assay for coronavirus detection using fast real-time RT-PCR”, iraqi journal of agricultural sciences, 51(2), pp. 556–564.
Salih W. and Yousif A.A. (2018b). Prevalence of salmonellosis in domestic dogs. Online Journal of Veterinary Research. 22 (9):739-743.
Salih W. and Yousif A.A.(2018a). Molecular detection of Salmonella typhimurium isolated from canine feces by PCR. Advances in Animal and Veterinary Science. 6 (12) 542- 547.
Sasi Kumar S, Gopinath S, Kalaichezhian M, Harish M, (2021), Surgical Management of Patients During Covid 19 Pandemic, Journal of Angiotherapy, 6(1), xx-xx
Smith C S, Lenz M F, Caldwell K, Oakey J. 2022. Identification of a canine coronavirus in Australian racing Greyhounds. Journal of Veterinary Diagnostic Investigation 2022, Vol. 34(1) 77– 81.
Soma, T., Hara, M., Ishii, H., Yamamoto, S. (2001) Antibody testing against Canine Coronavirus by immunoperoxidase plaque staining. Vet. Res. Commun. 25: 327-336.
Sulehria, M U; Ahmad, S S; Ijaz, M; Mushtaq, M H; Khan, A Y; Ghaffar, A. (2020). Molecular evidence and hematological alterations associated with the occurrence of coronavirus in domestic dogs in Pakistan. Tropical Biomedicine 37(4): 963–972.
Tadesse M, Ayana D, Kumsa B, Fromsam F. Zoonotic helminth parasites of dog in Bishoftu Town, central Ethiopia: prevalence, dog owners’ knowledge and control practice. J Ethiop Vet. 2020; 24(1): 93-115
Takano, T., Yamashita S., Murata-Ohkubo M., Satoh K., Doki T., and Hohdatsu T. Prevalence of canine coronavirus (CCoV) in dogs in Japan: detection of CCoV RNA and retrospective serological analysis. J. Vet. Med. Sci., 78(2): 341–345 (2016).
Tekelioglu BK, Yüceer HB, Akin B, Koç Ö, Çelik M, Kandir S, Baykal Çelik L, Gökçe M. 2021. Osmaniye Ilinde Sahipsiz Köpeklerin Rehabilitasyonu ve Viral Enfeksiyon Profilaksisi. Avrupa Bilim ve Teknoloji Dergisi, Ejosat, Özel Sayi 32: 956-966.
Tekelioglu, B., K. (2022). Seroepidemiological and Clinicopathological Investigation of Canine Coronavirus Infection in Dogs, in Türkiye. Turkish Journal of Agriculture - Food Science and Technology, 10(10): 1884-1892.
Warembourg C, Wera E, Odoch T, Bulu PM, Berger-González M, Alvarez D, et al. Comparative study of free-roaming domestic dog management and roaming behavior acrossfour countries: Chad, Guatemala, Indonesia, and Uganda. Front Vet Sci. 2021; 8:A617900.
Yagi K., Holowaychuk M. K. (2016). Manual of Veterinary Transfusion Medicine and Blood Banking (First Edition). WILEY Blackwell. USA; pp; 199.
Yesilbag K, Yilmaz Z, Torun S, Pratelli A. 2004. Canine coronavirus infection in Turkish dog population. Journal of Veterinary Medicine, Infectious Diseases and Veterinary Public Health, 51(7): 353–355.
Ye?ilbag, K., Yilmaz, Z., Torun, S., Pratelli, A., 2004. Canine coronavirus infection in Turkish dog population. J. Vet. Med. B Infect. Dis Vet. Public Health 51, 353–355.
Yilmaz, V. (2017). Seroprevalence of Canine Coronavirus in Kars Shepherd Dogs in Kars Province, Turkey. Palestine Journal of Veterinary Medicine. Vol. 72 (1)
Yoon SJ, Seo KW, Song KH. 2018. Clinical evaluation of a rapid diagnostic test kit for detection of canine coronavirus, Korean Journal of Veterinary Research, 58: 27–31.
Yousif A. A., Hasan M S. and Alwan M J. (2016). Clinical and molecular study of E. coli O157:H7 isolated from Diarrheic and non-diarrheic dogs. MRVSA 5 (2), 1-10
Yousif A.A., Hasan M S., Najim T M. and Hussein M A. (2017). Detection of virulence and antimicrobial susceptibility testing of Escherichia coli O157:H7 isolated from pets and stray dogs. Journal of Entomology and Zoology Studies 5(4): 573-578
Zahra A. Kheudhier, Alaa K. Mossa, (2022), Ferritin and High Sensitivity C-Reactive Protein (hs-CRP) may predict the COVID-19 patient’s fate, Journal of Angiotherpay, 6(2)
Zhou YH, Yin JB, Liu L, Yin XP, Li YM, Zhang ZD (2020). Prokaryotic expression of canine coronavirus N protein and preparation of its polyclonal antibodies. Chinese Animal Husbandry & Veterinary Medicine.
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