Nutmeg (Myristica fragrans) Extracts Accelerate Oral Mucosal Wound Healing In Vivo
Dewi Rosmalia 1, Minarni 2, Mhd Riza Marjoni 2*
Journal of Angiotherapy 8(10) 1-6 https://doi.org/10.25163/angiotherapy.8109929
Submitted: 01 July 2024 Revised: 25 September 2024 Published: 04 October 2024
Abstract
Background: Oral mucosal pain and sores are common concerns in dental care, often resulting from surgeries, infections, trauma, or autoimmune disorders. Conventional treatments like NSAIDs offer relief but carry adverse side effects. Nutmeg (Myristica fragrans) has been traditionally used for its analgesic, anti-inflammatory, and antimicrobial properties. However, its potential in oral mucosal wound healing remains underexplored. Methods: This study utilized male Wistar rats (Rattus norvegicus) to assess the wound healing effects of nutmeg extracts and fractions. Nutmeg was extracted using maceration, and the fractions were obtained through liquid-liquid extraction. Rats were divided into control and treatment groups, and topical nutmeg treatments were applied daily to incised gingival mucosa. Wound closure time was monitored daily, and healing rates were analyzed. Results: The ethyl acetate fraction of nutmeg exhibited the fastest wound healing, achieving an average healing rate of 88.79%, closely matching the positive control (94.65%). Other fractions also demonstrated efficacy, with the aqueous fraction achieving 82.46% and the ethanol fraction 75.54%. The hexane fraction showed the slowest healing at 63.67%. Statistical analysis revealed significant differences between the groups (p < 0.05). Conclusion: Nutmeg extracts, particularly the ethyl acetate fraction, significantly accelerated wound healing in the oral mucosa of Wistar rats, highlighting its potential as a natural wound-healing agent. Further research is needed to isolate the bioactive compounds responsible for these effects and to explore their clinical applications in dental care.
Keywords: Nutmeg, Wound healing, Oral mucosa, Bioactive compounds, Dental care
References
Akintoye, S., & Greenberg, M. S. (2014). Recurrent aphthous stomatitis. Dental Clinics of North America, 58(2), 281-297. https://doi.org/10.1016/j.cden.2013.12.002
Amin, A. et al. (2022). "Anti-inflammatory effects of lignans and neolignans from nutmeg." Journal of Natural Products, 85(4), 634-642.
Aoki, T., Kawahara, T., & Yamaguchi, M. (2019). Safety and efficacy of immediate-release omeprazole and amoxicillin twice daily for 1 week in the treatment of Helicobacter pylori infection in adults in Japan: A phase III, randomized, double-blind, multicenter study. Journal of Gastroenterology, 54(9), 792-802. https://doi.org/10.1007/s00535-019-01575-w
Bhatnagar, S., Saxena, M., Sandhir, M., & Bhardwaj, G. (2018). Management of oral mucosal lesions: A review. International Journal of Applied Dental Sciences, 4(4), 321-326.
Chatterjee, P. et al. (2020). "Antimicrobial activity of lignans and neolignans from nutmeg." Journal of Ethnopharmacology, 250, 112483.
Gupta, R. et al. (2021). "Antioxidant properties of nutmeg-derived compounds in wound healing." Journal of Medicinal Chemistry, 64(22), 16487-16498.
Habiboglu, S. and Basri, H. (2020). "Natural products in wound healing: A review." Phytotherapy Research, 34(6), 1235-1250.
Kizil, M., Khunt, O., Williams, J., & Patterson, R. (2017). Altered composition of the essential oil of Myristica fragrans (Houtt.) due to botanical predators. American Journal of Essential Oils and Natural Products, 5(1), 1-7.
Kotlia, S. and Singh, N. (2015). "Lignans and neolignans as bioactive compounds in nutmeg." Phytochemistry, 117, 236-245.
Mbabi, S., Williams, R., Rietveld, S., & Kusters, J. (2021). Nutmeg: A potential therapeutic plant for the management of pain and inflammation. Molecules, 26(21), 6483. https://doi.org/10.3390/molecules26216483
Ozaki, Y., Kawahara, T., & Yamaguchi, M. (2019). Antiallodynic effect of nutmeg essential oil in neuropathic pain mice model. Journal of Natural Medicines, 73(1), 187-195. https://doi.org/10.1007/s11418-018-1256-5
Rosmalia, D., Minarni, & Marjoni, M. R. (2022). Effect of nutmeg (Myristica fragrans) methanolic extract to the growth of dental plaque bacteria. DENTA, 16(2), 74-80. https://doi.org/10.30649/denta.v16i2.3
Salehi, B. et al. (2019). "Nutmeg: Traditional uses and modern therapeutic potential." Biomolecules, 9(1), 34.
Takzaree, N. et al. (2016). "The role of nutmeg in promoting collagen and IGF-1 expression during wound healing." Cell Proliferation, 49(5), 549-556.
Thakur, D., Jain, P., & Narang, S. (2021). Nutmeg: A potential therapeutic agent for the management of pain and inflammation. Phytotherapy Research, 35(11), 6144-6162. https://doi.org/10.1002/ptr.7204
Zhang, Y., Ozaki, Y., Kawahara, T., & Yamaguchi, M. (2020). Anti-inflammatory and analgesic effects of α-sabinene, a natural monoterpene isolated from nutmeg. Biomedicine & Pharmacotherapy, 134, 111172. https://doi.org/10.1016/j.biopha.2020.111172
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