A Review of Healing Potential of Indian Flora: An Ethnopharmacological Evidence of Wound Healing Activity

Rasmita Jena ^1,2, Durga Madhab Kar ^1*, Sovan Pattanaik ¹, Bhisma Narayan Ratha ³

This review emphasizes the rich potential in traditional practices, offering valuable insights and solutions to current healing challenges.

Abstract

Introduction: Healing a wound involves a complex series of coordinated chemical and cellular events to restore the integrity of skin and underlying tissues. Many people prefer using medicinal plants as an alternative therapy for this purpose globally. Objective: The focal point of the review is based on the ethnopharmacological profiling and validation of plants. The reviewed plants belong to the India region and have promising wound healing activity which has been scientifically proven by in vivo as well as in vitro studies. Numerous phyto extracts and their secondary metabolites are known as promising alternatives for wound healing agents due to the existence of miscellaneous dynamic constituents, ease of access, and their inadequate side effects. Methods: The plant profile was explored from various sources including scientific search engines such as Google Scholar, Niscare repository, Scopus, Pubmed, Sciencedirect, Sci-Finder, and Springer including reports and thesis from Proquest. Keywords such as wound healing property, plant extract, and animal models were employed in combination with the ethnopharmacological claim, pharmacological activity, etc. Conclusion: This review provides comprehensive data from published research work on medicinal plants over 10 years, focusing on wound healing activity and its ethnopharmacological profiling from different tribal belts. This review features the potential of medicinal plants to contribute to drug discovery as a compelling wound healer which may help in the discovery of a new drug molecule with a maximum margin of safety. However further studies are needed such as novel formulation, and target-based drug delivery systems by performing clinical studies for the scientific exploration and validation from laboratory to industry for societal value.

Keywords: Wound healing, natural product, phyto-medicine, ethnopharmacology, organic composites.

References

Acharya, N., Acharya, S., Shah, U., Shah, R., Hingorani, L., 2016. A comprehensive analysis on Symplocos racemosa Roxb .: Traditional uses , botany , phytochemistry and pharmacological activities. J. Ethnopharmacol. 181, 236–251. https://doi.org/10.1016/j.jep.2016.01.043

Ahmed, H.M., 2023. Ethnopharmacology of medicinal plants used in choman community , Hewler ( Erbil ), Kurdistan Region , Iraq. Genet. Resour. Crop Evol. https://doi.org/10.1007/s10722-023-01800-8

Alankar shrivastav, Arun Kumar Mishra, Syed Salman Ali, Aftab Ahmad, mohammed F. Abuzinadah, N.A.K. e tal., 2018. In vivo models for assesment of wound healing potential: Asystematic review. wound Med. 20, 43–53. https://doi.org/.1037//0033-2909.I26.1.78

Anima, P., Arun, M., Satish, S., 2019. Scientific validation of wound healing potential of Jasminum sambac Ait. South African J. Bot. 121, 584–589. https://doi.org/10.1016/j.sajb.2018.11.018

Ayyanar, M., Ignacimuthu, S., 2009. Herbal medicines for wound healing among tribal people in Southern India: Ethnobotanical and scientific evidences. Int. J. Appl. Res. Nat. Prod. 2, 29–42.

B.V.B., B., H.S., V., H.V., S., Shyam Prasad, K., 2017. Hydroalcoholic extract from Lepidium meyenii (Black Maca) root exerts wound healing activity in Streptozotocin-induced diabetic rats. Wound Med. 19, 75–81. https://doi.org/10.1016/j.wndm.2017.10.003

Bahramsoltani, R., Farzaei, M.H., Rahimi, R., 2014. Medicinal plants and their natural components as future drugs for the treatment of burn wounds?: an integrative review. Arch. Dermacology Res. 36, 601–617. https://doi.org/10.1007/s00403-014-1474-6

Boakye, Y.D., Agyare, C., Ayande, G.P., Titiloye, N., Asiamah, E.A., Danquah, K.O., 2018. Assessment of wound-healing properties of medicinal plants: The case of Phyllanthus muellerianus. Front. Pharmacol. 9, 1–12. https://doi.org/10.3389/fphar.2018.00945

Broughton, G., Janis, J.E., Attinger, C.E., 2006. A brief history of wound care. Plast. Reconstr. Surg. 117, 6–11. https://doi.org/10.1097/01.prs.0000225429.76355.dd

D. Senthil Rajan, M. Rajkumar, C.T.K. and K.L.S.K., 2013. Wound healing activity of an herbal ointment containing the leaf extract of Ziziphus Mauritiana Lam. African J. Pharm. Pharmacol. 7, 98–103. https://doi.org/10.5897/ajpp12.795

Dev, S.K., Choudhury, P.K., Srivastava, R., Sharma, M., 2019. Antimicrobial, anti-inflammatory and wound healing activity of polyherbal formulation. Biomed. Pharmacother. 111, 555–567. https://doi.org/10.1016/j.biopha.2018.12.075

Dinh, T., Braunagel, S., Rosenblum, B.I., 2015. G ro w t h F a c t o r s in Wo u n d Healing The Present and the Future?? Growth Factors Wound Heal. 32, 109–119.

Dwivedi, D., Dwivedi, M., Malviya, S., Singh, V., 2017. Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J. Tradit. Complement. Med. 7, 79–85. https://doi.org/10.1016/j.jtcme.2015.12.002

Firdous, S.M., Sautya, D., 2018. Medicinal plants with wound healing potential. Bangladesh J. Pharmacol. 13, 41–52. https://doi.org/10.3329/bjp.v13i1.32646

Ghosh, D., Mondal, S., Ramakrishna, K., 2019. A topical ointment formulation containing leaves extract of Aegialitis rotundifolia Roxb., accelerates excision, incision and burn wound healing in rats. Wound Med. https://doi.org/10.1016/j.wndm.2019.100168

Gowdru, H.B., Rajashekarappa, S., Manjunatha, H., 2012. Molecular docking and evaluation of wound healing activity of stigmastatone isolated from bark of Celastrus paniculatus Willd . J. Biochem. Technol. Technol. 3, 134–137.

Gupta, A., Kumar, P., 2015. Assessment of the histological state of the healing wound. Plast. Aesthetic Res. 2, 239. https://doi.org/10.4103/2347-9264.158862

Hajialyani, M., Tewari, D., Sobarzo-Sánchez, E., Nabavi, S.M., Farzaei, M.H., Abdollahi, M., 2018. Natural product-based nanomedicines for wound healing purposes: Therapeutic targets and drug delivery systems. Int. J. Nanomedicine 13, 5023–5043. https://doi.org/10.2147/IJN.S174072

Hiwa M.Ahmed, Seyed Nabavi, S.B., 2021. Herbal Drugs and Natural Products in the light of Nanotechnology and Nanomedicine for Developing Drug Formulations. Mini-Reviews Med. Chem. 21, 302–313. https://doi.org/10.2174/1389557520666200916143240

Imran, H., Saleem, U., Anwar, F., Khan, Z., Saleem, F., Ahmad, B., Waqar, A.B., 2023. Evaluation of angiogenic and wound healing activity of bergenia ciliata rhizome?: in ovo and in vivo study using histological and analytical tools. J. Anim. Plant Sci. 33, 805–816. https://doi.org/https://doi.org/10.36899/JAPS.2023.4.0674

Jahandideh, M., Hajimehdipoor, H., Mortazavi, S.A., Dehpour, A., Hassanzadeh, G., 2017. Evaluation of the wound healing activity of a traditional compound herbal product using rat excision wound model. Iran. J. Pharm. Res. 16, 153–163.

Jamkhande, Prasad G., A.S.W., 2015. Annona reticulata Linn. (Bullock’s heart)_ Plant profile, phytochemistry and pharmacological properties _ Elsevier Enhanced Reader.pdf. J. Tradit. Complement. Med. 5, 144–152.

Jones, M.L., 2015. A short history of the development of wound care dressings. Br. J. Healthc. Assist. 9, 482–485. https://doi.org/10.12968/bjha.2015.9.10.482

Joshua S. Boateng et al., 2008. Wound Healing Dressings and Drug Delivery Systems?: A Review. J. Pharm. Sci. 97, 2892–2923. https://doi.org/DOI 10.1002/jps.21210

K, M.K., Vittalrao, A.M., Se, P.K., Prabhath, S., 2020. Evaluation of Wound Healing Activity of an Ethanolic Extract of Anacardium occidentale Leaves in Wistar Rats. Biomed. Pharmacol. J. 13, 2061–2068. https://doi.org/https://dx.doi.org/10.13005/bpj/2086

Kriti Juneja, Rutusmita Mishra, Samrat Chauhan, Sumeet Gupta, P.R., 2019. metabolite profiling and wound healing activity of Boerhavia diffusa leaf extrats using in vitro and in vivo models. J. Tradit. Complement. Med. article in, 1–9. https://doi.org/.1037//0033-2909.I26.1.78

L. Azmi, I. Shukla, A. Goutam, Allauddin, Ch. V. Rao, T. Jawaid, M. Kalmal, a. S. Awaad, S I. Alqasoumi, O.A.A., 2019. In vitro wound healing activity of 1-hydroxy-5,7-dimethoxy-2-naphthalene-carboxaldehyde (HDNC) and other isolates of Aegle marmelos L._ Enhances keratinocytes motility via Wnt_Î2-catenin and RAS-ERK pathways. Saudi Pharm. J. 27, 532–539. https://doi.org/https://doi.org/10.1016/J.jsps.2019.01.017

Lazarus, G.S., Cooper, D.M., Knighton, D.R., Margolis, D.J., Percoraro, R.E., Rodeheaver, G., Robson, M.C., 1994. Definitions and guidelines for assessment of wounds and evaluation of healing. Wound Repair Regen. 2, 165–170. https://doi.org/10.1046/j.1524-475X.1994.20305.x

Manikandan, R., Anjali, R., Beulaja, M., Prabhu, N.M., Koodalingam, A., Saiprasad, G., Chitra, P., Arumugam, M., 2019. Synthesis, characterization, anti-proliferative and wound healing activities of silver nanoparticles synthesized from Caulerpa scalpelliformis. Process Biochem. 79, 135–141. https://doi.org/10.1016/j.procbio.2019.01.013

Mazumdar, S., Ghosh, A.K., Dinda, M., Das, A.K., Das, S., Jana, K., Karmakar, P., 2021. Journal of Traditional and Complementary Medicine Evaluation of wound healing activity of ethanol extract of Annona reticulata L . leaf both in vitro and in diabetic mice model. J. Tradit. Complement. Med. 11, 27–37. https://doi.org/https://doi.org/10.1016/j.jtcme.2019.12.001

Nagar, H.K., Srivastava, A.K., Srivastava, R., Kurmi, Madan Lal, Harinarayan Singh Chandel, M.S.R., 2016. Pharmacological Investigation of the Wound Healing Activity of Cestrum nocturnum ( L .) Ointment in Wistar Albino Rats. J. Pharm. 1–8. https://doi.org/http://dx.doi.org/10.1155/2016/9249040

Negm, S.A.E.S.W.A., 2023. The wound healing effect of botanicals and pure natural substances used in in vivo models. Inflammopharmacology 31, 755–772.

Orsted, H.L., 2018. Skin: Anatomy, Physiology and Wound Healing. Can. Assoc. Wound Care.

Paswan, S.K., Srivastava, S., Rao, C., 2020. Wound Healing Activity of Ethanolic Extract of Selaginella Bryopteris on Rats. Pharmacogn. J. 12, 335–341. https://doi.org/DOI?: 10.5530/pj.2020.12.53 Article

Prasathkumar, M., Raja, K., Vasanth, K., Khusro, A., Sadhasivam, S., Sahibzada, M.U.K., Gawwad, M.R.A., Al Farraj, D.A., Elshikh, M.S., 2021. Phytochemical screening and in vitro antibacterial, antioxidant, anti-inflammatory, anti-diabetic, and wound healing attributes of Senna auriculata (L.) Roxb. leaves. Arab. J. Chem. 14, 1–13. https://doi.org/10.1016/j.arabjc.2021.103345

Ramya Devi, D., Sowmiya Lakshna, S., Veena Parvathi, S., Vedha Hari, B.N., 2018. Investigation of wound healing effect of topical gel of Albizia amara leaves extract. South African J. Bot. 119, 400–409. https://doi.org/10.1016/j.sajb.2018.10.005

Roy, I., , Karuppur Thiagarajan Magesh , Mayilvakanam Sathyakumar , Annamalai Sivachandran, n Deenadayalan Purushothaman, R.A., 2023. Evaluation of Wound Healing Property of the Ethanolic Extract of Glycyrrhiza Glabra on Vero Cell Lines Using In Vitro Scratch assay Test. J. Pharm. Bioallied Sci. 15, 630–635. https://doi.org/10.4103/jpbs.jpbs

Sayeed Mohammed, D.S., 2018. Medicinal plants with wound healing potential. A J. Bangladesh Pharmacol. Soc. 13, 41–52. https://doi.org/10.3329/bjp.v13i1.32646

Shaibi, K.M.M., Leeba, B., Jamuna, S., Babu, R., 2022. Phytochemical Analysis , In Vitro Antioxidant , and Wound Healing Activities of Turbinaria ornata ( Turner ) J . Agardh from Gulf of Mannar, India. Appl. Biochem. Biotechnol. 194, 395–406. https://doi.org/10.1007/s12010-021-03752-0

Shedoeva, A., Leavesley, D., Upton, Z., Fan, C., 2019. Wound Healing and the Use of Medicinal Plants. Evidence-based Complement. Altern. Med. 2019, 1–30. https://doi.org/https://doi.org/10.1155/2019/2684108

Siddique, Z., Shah, G.M., Ahmed, H.M., Nisa, S., Khan, A., Idrees, M., Naz, S., Hassan, S.W., Mohiuddin, M., 2019. Ethnophytotherapy Practices for Wound Healing among Populations of District Haripur , KPK , Pakistan. Evidence-based Complement. Altern. Med. 2019, 1–11. https://doi.org/https://doi.org/10.1155/2019/4591675

Stuart Enoch, D.J.L., 2010. Basic science of wound healing. Surgery 23, 37–42. https://doi.org/10.1016/j.mpsur.2010.05.007

Subramanian, S., Duraipandian, C., Alsayari, A., Ramachawolran, G., Wong, L.S., Sekar, M., Gan, S.H., Subramaniyan, V., Seethalakshmi, S., Jeyabalan, S., Dhanasekaran, S., Chinni, S. V., Mat Rani, N.N.I., Wahab, S., 2023. Wound healing properties of a new formulated flavonoid-rich fraction from Dodonaea viscosa Jacq. leaves extract. Front. Pharmacol. 14, 1–16. https://doi.org/10.3389/fphar.2023.1096905

Tauber, A.I., 2003. Metchnikoff and the phagocytosis theory. Nat. Rev. Mol. Cell Biol. 4, 897–901. https://doi.org/10.1038/nrm1244

Upadhyay, A., Chattopadhyay, P., Goyary, D., Mazumder, P.M., Veer, V., 2014. Ixora coccinea Enhances Cutaneous Wound Healing by Upregulating the Expression of Collagen and Basic Fibroblast Growth Factor. ISRN Pharmacol. 2014, 1–9. https://doi.org/10.1155/2014/751824

Velnar, T., Bailey, T., Smrkolj, V., 2009. The Wound Healing Process?: an Overview of the Cellular and Molecular Mechanisms. J. Int. Med. Res. 37, 1528–1542. https://doi.org/10.1177/147323000903700531

Yadav, E., Singh, D., Yadav, P., Verma, A., 2018. Antioxidant and anti-inflammatory properties of Prosopis cineraria based phenolic rich ointment in wound healing. Biomed. Pharmacother. https://doi.org/10.1016/j.biopha.2018.09.180

Yogesh P. Talekar, Kishori G. Apte, Shubhangi V. Paygude, Prasad R. Tondare, P.B.P., 2017. Studies on wound healing potential of polyherbal formulation using invitro and invivo assay. J. Ayurveda Integr. Med. 8, 73–81. https://doi.org/dx.doi.org/10.1016/j.jaim.2016.11.007