Serum miRNAs as Potential Diagnostic Biomarkers for Non-Obstructive Azoospermia
Fatima Mahdi Kadhum 1*, Ali Ibrahim Rahim 2, Ula Al-Kawaz 1
Journal of Angiotherapy 8(4) 1-5 https://doi.org/10.25163/angiotherapy.849582
Submitted: 03 February 2024 Revised: 04 April 2024 Published: 08 April 2024
Abstract
Background: Infertility in couples shows a significant challenge worldwide, with non-obstructive azoospermia (NOA) being a prevalent condition characterized by defective spermatogenesis. Research into genetic factors contributing to NOA and the exploration of non-invasive diagnostic biomarkers are crucial for effective management. Method: Thirty-five NOA men underwent testicular biopsy and physical assessments. Serum miRNA (miR-211, miR-429, miR-34c-5p) levels were measured using RT-PCR to evaluate their potential as diagnostic biomarkers. Results: Among the patients, 77.14% showed positive biopsy results. Serum miR-211 levels were significantly lower in NOA patients compared to controls (p < 0.001), while miR-429 and miR-34c-5p showed slight decreases without significance. ROC analysis revealed diagnostic potential for miR-211. Discussion: Dysregulation of miRNAs, particularly miR-211, has been associated with spermatogenesis disorders. However, limited research exists on miRNA profiles in NOA. Our findings align with previous studies, suggesting miRNA-211's potential as a biomarker. Further studies with larger cohorts are warranted. Conclusion: Serum miR-211 demonstrates promise as a non-invasive diagnostic biomarker for NOA. This study underscores the importance of miRNAs in male infertility diagnostics and lays the groundwork for future research in this area. Developing non-invasive diagnostic techniques is crucial for effective management of male infertility.
Keywords: Non-obstructive azoospermia, miRNA biomarkers, male infertility, diagnostic technique, spermatogenesis, miR-211, miR-34c-5p, and miRNA-429
References
Cao, Y., Wang, H., Jin, Z., Hang, J., Jiang, H., Wu, H., & Zhang, Z. (2023). Characterization of Non-Obstructive Azoospermia in Men Using Gut Microbial Profiling. Journal of Clinical Medicine, 12(2), 701.
Chen, J., & Han, C. (2023). In vivo functions of miRNAs in mammalian spermatogenesis. Frontiers in Cell and Developmental Biology, 11, 1154938.
Fu, J., Imani, S., Wu, M. Y., & Wu, R. C. (2023). MicroRNA-34 Family in Cancers: Role, Mechanism, and Therapeutic Potential. Cancers, 15(19). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10571940/
Khawar, M. B., Mehmood, R., & Roohi, N. (2019). MicroRNAs: Recent insights towards their role in male infertility and reproductive cancers. Bosnian Journal of Basic Medical Sciences, 19(1), 31. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6387678/
Krausz, C., & Cioppi, F. (2021). Genetic Factors of Non-Obstructive Azoospermia: Consequences on Patients’ and Offspring Health. Journal of Clinical Medicine, 10(17). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8432470/
Li, H., Xiao, X., Zhang, J., Zafar, M. I., Wu, C., Long, Y., Lu, W., Pan, F., Meng, T., Zhao, K., Zhou, L., Shen, S., Liu, L., Liu, Q., & Xiong, C. (2020). Impaired spermatogenesis in COVID-19 patients. EClinicalMedicine, 28.
Pantos, K., Grigoriadis, S., Tomara, P., Louka, I., Maziotis, E., Pantou, A., Nitsos, N., Vaxevanoglou, T., Kokkali, G., Agarwal, A., Sfakianoudis, K., & Simopoulou, M. (2021). Investigating the Role of the microRNA-34/449 Family in Male Infertility: A Critical Analysis and Review of the Literature. Frontiers in Endocrinology, 12. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8281345/
Pop, M. A., & Almquist, B. D. (2021). Controlled Delivery of MicroRNAs into Primary Cells Using Nanostraw Technology. Advanced NanoBiomed Research, 1(6), 2000061. https://onlinelibrary.wiley.com/doi/full/10.1002/anbr.202000061
Saebnia, N., Neshati, Z., & Bahrami, A. R. (2021). Role of microRNAs in etiology of azoospermia and their application as non-invasive biomarkers in diagnosis of azoospermic patients. Journal of Gynecology Obstetrics and Human Reproduction, 50(10), 102207.
Shapiro, M., & Ohlander, S. (2018). Endocrine stimulatory therapy for testis sperm extraction. In Encyclopedia of Reproduction (pp. 318–323).
Vander Borght, M., & Wyns, C. (2018). Fertility and infertility: Definition and epidemiology. Clinical Biochemistry, 62, 2–10. https://pubmed.ncbi.nlm.nih.gov/29555319/
Ye, L., Wang, F., Wang, J., Wu, H., Yang, H., Yang, Z., & Huang, H. (2022). Role and mechanism of miR-211 in human cancer. Journal of Cancer, 13(9), 2933. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9330454/
Zhang, J., Campion, S., Catlin, N., Reagan, W. J., Palyada, K., Ramaiah, S. K., & Ramanathan, R. (2023). Circulating microRNAs as promising testicular translatable safety biomarkers: current state and future perspectives. Archives of Toxicology, 97(4), 947. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9933818/
Zhang, Y., Tang, Y., Huang, J., Liu, H., Liu, X., Zhou, Y., Ma, C., Wang, Q., Yang, J., Sun, F., & Zhang, X. (2022). Circulating microRNAs in seminal plasma as predictors of sperm retrieval in microdissection testicular sperm extraction. Annals of Translational Medicine, 10(7), 392. https://atm.amegroups.org/article/view/89239/html
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