Serum miRNAs as Potential Diagnostic Biomarkers for Non-Obstructive Azoospermia
Fatima Mahdi Kadhum 1*, Ali Ibrahim Rahim 2, Ula Al-Kawaz 1
Journal of Angiotherapy 8(4) 1-8 https://doi.org/10.25163/angiotherapy.849582
Submitted: 03 February 2024 Revised: 04 April 2024 Published: 08 April 2024
Abstract
Background: Infertility in couples shows a significant challenge worldwide, with non-obstructive azoospermia (NOA) being a prevalent condition characterized by defective spermatogenesis. Research into genetic factors contributing to NOA and the exploration of non-invasive diagnostic biomarkers are crucial for effective management. Method: Thirty-five NOA men underwent testicular biopsy and physical assessments. Serum miRNA (miR-211, miR-429, miR-34c-5p) levels were measured using RT-PCR to evaluate their potential as diagnostic biomarkers. Results: Among the patients, 77.14% showed positive biopsy results. Serum miR-211 levels were significantly lower in NOA patients compared to controls (p < 0.001), while miR-429 and miR-34c-5p showed slight decreases without significance. ROC analysis revealed diagnostic potential for miR-211. Discussion: Dysregulation of miRNAs, particularly miR-211, has been associated with spermatogenesis disorders. However, limited research exists on miRNA profiles in NOA. Our findings align with previous studies, suggesting miRNA-211's potential as a biomarker. Further studies with larger cohorts are warranted. Conclusion: Serum miR-211 demonstrates promise as a non-invasive diagnostic biomarker for NOA. This study underscores the importance of miRNAs in male infertility diagnostics and lays the groundwork for future research in this area. Developing non-invasive diagnostic techniques is crucial for effective management of male infertility.
Keywords: Non-obstructive azoospermia, miRNA biomarkers, male infertility, diagnostic technique, spermatogenesis, miR-211, miR-34c-5p, and miRNA-429
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