Microbial Bioactives
Microbial Bioactives | Online ISSN 2209-2161
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Volume 7 Number 1 2024
REVIEWS (Open Access)
Advances in Biodiversity Exploration and Bioactive Metabolite Discovery: Insights from Cyanobacteria, Microalgae, and Uncultured Microorganisms
Rizwan Rashid Bazmi1, Muhammad Asif2*, Hafiza Sidra Yaseen3 , Ashok Gnanasekaran5*
Microbial Bioactives 7 (1) 1-8 https://doi.org/10.25163/microbbioacts.7110660
Submitted: 22 September 2024 Revised: 17 November 2024 Accepted: 25 November 2024 Published: 27 November 2024
Abstract
Microorganisms, including cyanobacteria, microalgae, and uncultured bacteria, constitute a vast and largely unexplored reservoir of biodiversity and bioactive metabolites. Traditional culture-based methods have historically limited access to microbial diversity, capturing less than 1% of species, while leaving “microbial dark matter” untapped. Recent technological advancements in robotic sampling, high-resolution microscopy, high-throughput screening, and omics-based analyses have transformed our capacity to systematically explore microbial communities across diverse habitats, including deep-sea sediments, hydrothermal vents, acidic craters, and mesophotic ecosystems. These integrated approaches facilitate in situ preservation, detailed structural characterization, and functional assessment of microbial metabolites, enabling discovery of novel compounds with pharmacological and industrial potential. High-throughput extraction and screening platforms accelerate identification of bioactive molecules, while single-cell genomics and metagenomics reveal cryptic biosynthetic gene clusters in previously uncultured organisms. Cryo-electron microscopy and atomic force microscopy provide nanometer-scale visualization of cellular architectures, photosynthetic complexes, and extracellular interactions, linking morphology to ecological function. Despite these advances, challenges remain in functionally annotating novel genes, reproducing environmental conditions, and optimizing hit rates in large-scale screening. Systematic integration of robotics, imaging, genomics, and metabolomics enables a holistic understanding of microbial ecosystems, supporting meta-analytical synthesis of trends in metabolite discovery and biodiversity patterns. This review highlights the state-of-the-art methodologies that uncover microbial diversity, offering insights into sustainable bioengineering, natural product discovery, and ecological monitoring.
Keywords: microbial diversity, cyanobacteria, microalgae, uncultured bacteria, high-throughput screening, metagenomics, bioactive metabolites
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