Microbial Bioactives
Microbial Bioactives | Online ISSN 2209-2161
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Volume 5 Number 2 2022
REVIEWS (Open Access)
Threshold-Dependent Effects of Lead (Pb) on Soil Microbial Diversity and Denitrifying Function: A Systematic Review and Meta-Analytical Perspective
Rita S. Adam 1*
Microbial Bioactives 5 (2) 1-8 https://doi.org/10.25163/microbbioacts.5210707
Submitted: 12 March 2022 Revised: 10 May 2022 Accepted: 17 May 2022 Published: 19 May 2022
Abstract
Soil microbial communities are central to ecosystem functioning, regulating nutrient cycling, organic matter turnover, and soil fertility. However, these communities are increasingly threatened by heavy metal contamination, particularly lead (Pb), a persistent and non-essential toxic element widely distributed due to industrialization, mining, urbanization, and agricultural activities. Although numerous studies have examined Pb toxicity in soils, reported effects on microbial diversity and function remain inconsistent, largely due to variations in soil properties, contamination levels, exposure duration, and methodological approaches. This systematic review and meta-analytical synthesis consolidates evidence from peer-reviewed studies published before 2023 to clarify how Pb contamination influences soil bacterial diversity, community composition, and denitrification-related functions. Across studies, increasing Pb concentrations were generally associated with reduced microbial richness and diversity, as indicated by Shannon and Chao1 indices, alongside pronounced shifts in community structure. Importantly, the analysis highlights threshold-dependent responses, where microbial communities exhibited relative stability at lower Pb concentrations but experienced rapid diversity loss and functional disruption beyond critical contamination levels. Denitrifying communities were particularly sensitive, reflecting the vulnerability of nitrogen-cycling processes to metal stress. Soil pH, organic matter content, and co-occurring metals emerged as key modulators of Pb bioavailability and microbial response, often buffering or intensifying toxicity. Evidence also suggests partial functional resilience driven by microbial redundancy and long-term adaptation in chronically contaminated soils. By integrating taxonomic and functional outcomes across diverse soil systems, this synthesis provides a clearer framework for understanding Pb-induced microbial stress, identifying ecological tipping points, and informing soil risk assessment, remediation strategies, and sustainable land management.
Keywords: Lead contamination; soil microbiome; microbial diversity; denitrification; heavy metals; systematic review; meta-analysis
References
Alengebawy, A., Abdelkhalek, S. T., Qureshi, S. R., & Wang, M. Q. (2021). Heavy metals and pesticides toxicity in agricultural soil and plants: Ecological risks and human health implications. Toxics, 9(3), 42. https://doi.org/10.3390/toxics9030042
Allison, S. D., & Martiny, J. B. H. (2008). Resistance, resilience, and redundancy in microbial communities. Proceedings of the National Academy of Sciences of the United States of America, 105(Suppl. 1), 11512–11519. https://doi.org/10.1073/pnas.0801925105
Beattie, R. E., Henke, W., Campa, M. F., Hazen, T. C., McAliley, L. R., & Campbell, J. H. (2018). Variation in microbial community structure correlates with heavy-metal contamination in soils decades after mining ceased. Soil Biology and Biochemistry, 126, 57–63. https://doi.org/10.1016/j.soilbio.2018.08.011
Bhakta, J. N., Lahiri, S., Bhuiyna, F. A., Rokunuzzaaman, M., Ohonishi, K., Iwasaki, K., & Jana, B. B. (2018). Profiling of heavy metal(loid)-resistant bacterial community structure by metagenomic-DNA fingerprinting using PCR-DGGE. Energy, Ecology and Environment, 3(1), 102–109. https://doi.org/10.1007/s40974-017-0079-2
Borenstein, M., Hedges, L. V., Higgins, J. P. T., & Rothstein, H. R. (2009). Introduction to meta-analysis. Wiley. https://doi.org/10.1002/9780470743386
Cui, H., Liu, L. L., Dai, J. R., Yu, X. N., Guo, X., Yi, S. J., & Du, N. (2018). Bacterial community shaped by heavy metals and contributing to health risks in cornfields. Ecotoxicology and Environmental Safety, 166, 259–269. https://doi.org/10.1016/j.ecoenv.2018.09.096
Delgado-Baquerizo, M., Maestre, F. T., Reich, P. B., et al. (2016). Microbial diversity drives multifunctionality in terrestrial ecosystems. Nature Communications, 7, 10541. https://doi.org/10.1038/ncomms10541
DerSimonian, R., & Laird, N. (1986). Meta-analysis in clinical trials. Controlled Clinical Trials, 7(3), 177–188. https://doi.org/10.1016/0197-2456(86)90046-2
Egger, M., Davey Smith, G., Schneider, M., & Minder, C. (1997). Bias in meta-analysis detected by a simple, graphical test. BMJ, 315, 629–634. https://doi.org/10.1136/bmj.315.7109.629
Egger, M., Davey Smith, G., Schneider, M., & Minder, C. (1997). Bias in meta-analysis detected by a simple, graphical test. BMJ, 315(7109), 629–634. https://doi.org/10.1136/bmj.315.7109.629
Fajardo, C., Costa, G., Nande, M., Botías, P., García-Cantalejo, J., & Martín, M. (2019). Pb, Cd, and Zn soil contamination: Monitoring functional and structural impacts on the microbiome. Applied Soil Ecology, 135, 56–64. https://doi.org/10.1016/j.apsoil.2018.10.022
Fierer, N. (2017). Embracing the unknown: Disentangling the complexities of the soil microbiome. Nature Reviews Microbiology, 15, 579–590. https://doi.org/10.1038/nrmicro.2017.87
Gao, P., Li, D., Liu, J., Yang, N., Gu, W., He, X., & Tang, W. (2019). Risk assessment and microbial community changes in farmland soil contaminated with heavy metals. Ecotoxicology and Environmental Safety, 185, 109685. https://doi.org/10.1016/j.ecoenv.2019.109685
Giller, K. E., Witter, E., & McGrath, S. P. (2009). Heavy metals and soil microbes. Soil Biology and Biochemistry, 41, 2031–2037. https://doi.org/10.1016/j.soilbio.2009.04.026
Gong, W.-J., Niu, Z.-F., Wang, X.-R., & Zhao, H.-P. (2021). How the soil microbial communities and activities respond to long-term heavy metal contamination in electroplating contaminated site. Microorganisms, 9(2), 362. https://doi.org/10.3390/microorganisms9020362
Griffiths, B. S., & Philippot, L. (2012). Insights into the resistance and resilience of the soil microbial community. FEMS Microbiology Reviews, 37, 112–129. https://doi.org/10.1111/j.1574-6976.2012.00343.x
Higgins, J. P. T., Thomas, J., Chandler, J., Cumpston, M., Li, T., Page, M. J., & Welch, V. A. (2022). Cochrane handbook for systematic reviews of interventions (Version 6.3). Cochrane. http://www.training.cochrane.org/handbook
Higgins, J. P. T., Thompson, S. G., Deeks, J. J., & Altman, D. G. (2003). Measuring inconsistency in meta-analyses. BMJ, 327(7414), 557–560. https://doi.org/10.1136/bmj.327.7414.557
Hu, H., Li, M., Wang, G., Drosos, M., Li, Z., Hu, Z., & Xi, B. (2019). Water-soluble mercury induced by organic amendments affected microbial community assemblage. Chemosphere, 236, 124405. https://doi.org/10.1016/j.chemosphere.2019.124405
Jousset, A., Bienhold, C., Chatzinotas, A., et al. (2017). Where less may be more: How the rare biosphere pulls ecosystems strings. ISME Journal, 11, 853–862. https://doi.org/10.1038/ismej.2016.174
Kuppusamy, S., Thavamani, P., Megharaj, M., & Naidu, R. (2016). Bioremediation of metal-contaminated soils. Environment International, 87, 1–15. https://doi.org/10.1016/j.envint.2015.10.018
Lin, Y., Ye, Y., Hu, Y., & Shi, H. (2019). Variation in microbial community structure under different heavy metal contamination levels. Ecotoxicology and Environmental Safety, 180, 557–564. https://doi.org/10.1016/j.ecoenv.2019.05.057
Liu, X., Ma, A., Zhuang, G., & Zhuang, X. (2018). Diversity of microbial communities involved in mercury methylation. MicrobiologyOpen, 7, e00577. https://doi.org/10.1002/mbo3.577
Luo, Z., Ma, J., Chen, F., Li, X., & Zhang, S. (2018). Effects of Pb smelting on the soil bacterial community near a secondary lead plant. International Journal of Environmental Research and Public Health, 15(5), 1030. https://doi.org/10.3390/ijerph15051030
Page, M. J., McKenzie, J. E., Bossuyt, P. M., Boutron, I., Hoffmann, T. C., Mulrow, C. D., et al. (2021). The PRISMA 2020 statement: An updated guideline for reporting systematic reviews. BMJ, 372, n71. https://doi.org/10.1136/bmj.n71
Shade, A., Peter, H., Allison, S. D., et al. (2012). Fundamentals of microbial community resistance and resilience. Frontiers in Microbiology, 3, 417. https://doi.org/10.3389/fmicb.2012.00417
Sobolev, D., & Begonia, M. F. T. (2008). Effects of heavy metal contamination upon soil microbes: Lead-induced changes in general and denitrifying microbial communities as evidenced by molecular markers. International Journal of Environmental Research and Public Health, 5(5), 450–456. https://doi.org/10.3390/ijerph5050450
Sterne, J. A. C., Sutton, A. J., Ioannidis, J. P. A., et al. (2011). Recommendations for examining funnel plot asymmetry. BMJ, 343, d4002. https://doi.org/10.1136/bmj.d4002
Tseng, S.-C., Liang, C.-M., Chia, T., & Ton, S.-S. (2021). Changes in the composition of the soil bacterial community in heavy metal-contaminated farmland. International Journal of Environmental Research and Public Health, 18(16), 8661. https://doi.org/10.3390/ijerph18168661
Violante, A., Cozzolino, V., Perelomov, L., Caporale, A. G., & Pigna, M. (2010). Mobility and bioavailability of heavy metals. Journal of Soil Science and Plant Nutrition, 173, 86–97. https://doi.org/10.4067/S0718-95162010000100005
Wyszkowska, J., Borowik, A., Zaborowska, M., & Kucharski, J. (2023). Sensitivity of Zea mays and soil microorganisms to the toxic effect of chromium (VI). International Journal of Molecular Sciences, 24(1), 178. https://doi.org/10.3390/ijms24010178
Zhao, X., Huang, J., Zhu, X., Chai, J., & Ji, X. (2020). Ecological effects of heavy metal pollution on soil microbial community structure and diversity on both sides of a river around a mining area. International Journal of Environmental Research and Public Health, 17(16), 5680. https://doi.org/10.3390/ijerph17165680
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