Antibiotic Sensitivity Pattern of Staphylococcus aureus Isolated from Pus Samples of Different Age and Sex Groups in Gazipur District, Bangladesh
Md. Eaktear Uddin 1, Shakila Sultana 1, Maruf Abony 1, Suvamoy Datta 1*
Journal of Primeasia 1.3(1) 1-5 https://doi.org/10.25163/primeasia.11560013
Submitted: 13 October 2020 Revised: 22 November 2020 Published: 03 December 2020
Abstract
Staphylococcus aureus has long been reported as one of the most important bacteria that cause different kinds of diseases in humans, particularly skin and soft tissue infections such as abscesses (boils), furuncles, and cellulitis. To treat these infections, certain antibiotics have frequently been used across the world. However, nowadays, there is a growing concern on the treatment of staphylococcal infections due to gaining resistance to some antibiotics by this bacterium. In the present work, S. aureus was isolated from 40 pus samples and a total of 40 isolates were selected to study their multi-drug resistance pattern using 10 available antibiotics. Among the antibiotics used, gentamycin was found to be the most effective to control S. aureus as the isolates showed minimum resistance to this antibiotic (0%). On the other hand, the isolates showed the highest resistance to amoxicillin (100%). Among 40 isolates, one isolate (2.5%) was resistant to the maximum number of antibiotics used in the experiments (seven), while five isolates (12.5%) showed resistance to maximum 6 antibiotics, and five isolates (12.5%) were resistant to at least 5 antibiotics. Overall, a total of 27.5% isolates were multi-drug resistant (resistant to 5 or more of the antibiotics tested). In conclusion, the findings of this study would be helpful to choose appropriate antibiotic for treating staphylococcal infections considering the multi-drug resistance of the bacterium.
Keywords: S. aureus, Pus samples, Gazipur city, Bangladesh,
References
Bartlett JG, Gilbert DN, Spellberg B 2013: Seven ways to preserve the miracle of antibiotics. Clinical infectious diseases 56(10): 1445-1450.
Bauer A 1966: Antibiotic susceptibility testing by a standardized single disc method. American Journal of clinical pathology 45: 149-15
Blot SI, Vandewoude KH, Hoste EA, Colardyn FA 2002: Outcome and attributable mortality in critically ill patients with bacteremia involving methicillin-susceptible and methicillin-resistant Staphylococcus aureus. Archives of Internal Medicine 162(19): 2229-2235.
Carter AP, Clemons WM, Brodersen DE, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V 2000: Functional insights from the structure of the 30S ribosomal subunit and its interactions with antibiotics. Nature 407(6802): 340-348.
Chang S, Sievert DM, Hageman JC, Boulton ML, Tenover FC, Downes FP, et al. 2003: Infection with vancomycin-resistant Staphylococcus aureus containing the vanA resistance gene. New England journal of medicine 348(14): 1342-1347.
Golkar Z, Bagasra O, Pace DG 2014: Bacteriophage therapy: a potential solution for the antibiotic resistance crisis. The Journal of Infection in Developing Countries 8(02): 129-136.
Herold BC, Immergluck LC, Maranan MC, Lauderdale DS, Gaskin RE, Boyle-Vavra S 1998: Community-acquired methicillin-resistant Staphylococcus aureus in children with no identified predisposing risk. Jama 279(8): 593-598.
Hiramatsu K, Hanaki H, Ino T, Yabuta K, Oguri T, Tenover F 1997: Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. The Journal of antimicrobial chemotherapy 40(1): 135-136.
Holden HM 1984: Molecular Structure of Kanamycin Nucleotidyl transferase Determined to 3.0-A Resolution. Biochemistry 1993(32): 11977-11971.
Hussain FM, Boyle-Vavra S, Bethel CD, Daum RS 2000: Current trends in community-acquired methicillin-resistant Staphylococcus aureus at a tertiary care pediatric facility. The Pediatric infectious disease journal 19(12): 1163-1166.
Johnson AP, Aucken HM, Cavendish S, Ganner M, Wale MC, Warner M, 2001: Dominance of EMRSA-15 and-16 among MRSA causing nosocomial bacteraemia in the UK: analysis of isolates from the European Antimicrobial Resistance Surveillance System (EARSS). Journal of Antimicrobial Chemotherapy 48(1): 143-144.
Lushniak BD 2014: Antibiotic resistance: a public health crisis. Public Health Reports 129(4): 314-316.
Mendem SK, Gangadhara TA, Shivannavar CT, Gaddad SM 2016: Antibiotic resistance patterns of Staphylococcus aureus: A multicenter study from India. Microbial pathogenesis 98: 167-170.
Menichetti F 2005: Current and emerging serious Gram-positive infections. Clinical Microbiology and Infection 11: 22-28.
Michael CA, Dominey-Howes D, Labbate M 2014: The antimicrobial resistance crisis: causes, consequences, and management. Frontiers in public health 2: 145.
Nature E 2013: The antibiotic alarm. Nature 495(7440): 141.
Pierzchala E, Ramos P, Pilawa B 2019: EPR study of free radicals formed in fusidic acid and neomycin under UV irradiation. Acta Poloniae Pharmaceutica-Drug Research 76(2): 215-223.
Read AF, Woods RJ 2014: Antibiotic resistance management. Evolution, medicine, and public health 2014(1): 147.
Reiner K 2010: Catalase test protocol. American society for microbiology: 1-6.
Rossolini G, Arena F, Pecile P, Pollini S 2014: Update on the antibiotic resistance crisis. Clin Opin Pharmacol.18: 56–60.
Ventola CL 2015: The antibiotic resistance crisis: part 1: causes and threats. Pharmacy and therapeutics 40(4): 277.
Viswanathan V 2014: Off-label abuse of antibiotics by bacteria. Gut microbes 5(1): 3-4.
Zhanel GG, Calic D, Schweizer F, Zelenitsky S, Adam H, Lagacé-Wiens PR 2010: New lipoglycopeptides. Drugs 70(7): 859-886.
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