Isolation, Identification and Antibiotic Susceptibility Analysis of Bacterial Pathogens from Suspected Urinary Tract Infected Patients of Tertiary Medical Centre in Dhaka City, Bangladesh
Md. Robeul Islam 1, Avijit Banik 1, Md. Abu Zihad 1, Kumkum Rahman Mouree 1* and Suvamoy Datta 1
Journal of Primeasia 1.3(1) 1-6 https://doi.org/10.25163/primeasia.11560012
Submitted: 02 October 2020 Revised: 11 November 2020 Published: 02 December 2020
Abstract
An infection of the urinary tract (kidneys, ureters, bladder, and urethra) is known as a urinary tract infection (UTI). The lower urinary tract, the bladder, and the urethra are the most often infected areas. Women are more likely than males to have a bacterial infection. The aim of the research was to determine the causative agent of UTI in patients and see how they responded to standard treatments. A total of 435 urine samples were examined using the culture technique. The samples were streaked evenly on blood agar, MacConkey, and then incubated for 24 hours at 37°C. The morphological characteristics of the colony on culture media were used to identify the presumptive bacterium. Gram staining and routine biochemical assays were also used to confirm the findings. On Muller-Hinton agar, the disk diffusion technique was employed to assess susceptibility to 12 different antibiotics. The most prevalent uropathogen was E. coli (44%) samples. The second isolate was Staphylococcus aureus (21%), Klebsiella species (13%) and Proteus species (12%), Enterobacter species (10%). In overall, the uropathogens were highest susceptible to Meropenem (82.2%), Amikacin (63.6%) and Cefixime (59.8%) highest resistance to Azithromycin (84.2%), Gentamycin (75.6%) and Nalidixic acid (64.4%). In both genders and age groups, E. coli was the most common uropathogen (44%). The most successful medications for the treatment of UTI Meropenem (82.2%), Amikacin (63.6%), and Azithromycin (93.15 percent), whereas Azithromycin (84.2%), Gentamycin (75.6%) and Nalidixic acid (64.4%) was the least effective. The present Study can be helpful for the clinicians in finding proper drugs in the developing countries like Bangladesh where multi-drug resistance problem has just complicated the treatment of UTIs.
Keywords: UTI, Uropathogen, Antibiotic Susceptibility, Tertiary Medical Centre.
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