Multidisciplinary research and review journal | Online ISSN 3064-9870
RESEARCH ARTICLE   (Open Access)

Isolation, Identification and Antibiotic Sensitivity Pattern of Salmonella typhi Isolated from Blood Samples of Patients in Dhaka City, Bangladesh

Mohammad Mizanur Rahman1, Maruf Abony1, Kaniz Fatema1, Suvamoy Datta1*

+ Author Affiliations

Journal of Primeasia 1.3(1) 1-5 https://doi.org/10.25163/primeasia.560014

Submitted: 11 October 2020  Revised: 05 December 2020  Published: 15 December 2020 

Abstract

In recent years, there has been a significant rise in the prevalence of multidrug resistance Salmonella typhi in Dhaka city. To study the prevalence rate and identification of typhoidal Salmonella typhi among the patients attending at a hospital by conventional test.  It was seen that 88% (Amoxyclave) of the S. typhi strains are resistant to the antibiotics used against them. Gentamycin, Levofloxacin and Meropenem (100% sensitive) are the best drugs for the treatment of S. typhi infection followed by Ciprofloxacin (99% sensitive). None of the samples exhibited sensitivity to more than four drugs. Typhoid fever occurs infrequently in developed counties but massively in developing countries. Traditionally the drugs of choice were chloramphenicol, ampicillin, and cotrimoxazole but unfortunately the emergence of multidrug resistant strains of S. typhi introduced the use of Amikacin, Imipenem & Netilmycin among the patients. However, now a day’s ciprofloxacin used frequently as a drug of choice but this study, isolated S. typhi are sensitive to ciprofloxacin 95 % that twisted the circumstances into different directions.

Keywords: Salmonella, Blood Samples, Antibiotic Sensitivity, Bangladesh.

References

 

Bauer A 1966: Antibiotic susceptibility testing by a standardized single disc method. American Journal of clinical pathology 45: 149-158.

Bäumler, A. J., Hargis, B. M., & Tsolis, R. M. (2000). Trick or treat: Salmonella interactions with host macrophages. Annual Review of Microbiology, 54(1), 203-231.

Bhutta, Z. A. (1996). Impact of age and drug resistance on mortality in typhoid fever. Archives of Disease in Childhood, 75(3), 214-217.

Bhutta, Z. A., & Dewraj, H. L. (2006). Current concepts in the diagnosis and treatment of typhoid fever. BMJ, 333(7558), 78-82.

Brenner, F. W., Villar, R. G., Angulo, F. J., Tauxe, R., & Swaminathan, B. (2000). Salmonella nomenclature. Journal of Clinical Microbiology, 38(7), 2465-2467.

Cao X, Kneen R, Nguyen T, Truong D, White N, Parry C 1999: A comparative study of ofloxacin and cefixime for treatment of typhoid fever in children. The Dong Nai Pediatric Center Typhoid Study Group. The Pediatric infectious disease journal 18(3): 245-248.

Cappuccino, J.G. and Sherman, N. 1996: Microbiology a Laboratory Manual. The Benjamin Cummings Publishing Co. Inc., San Francisco.

Chau TT, Campbell JI, Galindo CM, Van Minh Hoang N, Diep TS, Nga TTT 2007: Antimicrobial drug resistance of Salmonella enterica serovar Typhi in Asia and molecular mechanism of reduced susceptibility to the fluoroquinolones. Antimicrobial agents and chemotherapy 51(12): 4315-4323.

Chitnis V, Chitnis D, Verma S, Hemvani N 1999: Multidrug-resistant Salmonella typhi in India. The Lancet 354(9177): 514-515.

Crump, J. A., & Mintz, E. D. (2010). Global trends in typhoid and paratyphoid fever. Clinical Infectious Diseases, 50(2), 241-246.

Crump, J. A., Luby, S. P., & Mintz, E. D. (2004). The global burden of typhoid fever. Bulletin of the World Health Organization, 82(5), 346-353.

Eykyn S, Williams H 1987: Treatment of multi resistant Salmonella typhi with oral ciprofloxacin. The Lancet 330(8572): 1407-1408.

Glynn, M. K., Bopp, C., Dewitt, W., Dabney, P., Mokhtar, M., & Angulo, F. J. (1998). Emergence of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 infections in the United States. New England Journal of Medicine, 338(19), 1333-1338.

Gupta, S. K., Medalla, F., Omondi, M. W., Stroika, S., Whichard, J. M., & Fields, P. I. (2008). Laboratory-based surveillance of paratyphoid fever in the United States: travel and domestic acquisition. Clinical Infectious Diseases, 46(11), 1656-1663.

Kariuki, S., Gordon, M. A., Feasey, N., & Parry, C. M. (2015). Antimicrobial resistance and management of invasive Salmonella disease. Vaccine, 33, C21-C29

Keddy, K. H., Sooka, A., Smith, A. M., Musekiwa, A., & Tau, N. P. (2015). Typhoid fever in South Africa in an endemic HIV setting. PLoS One, 10(12), e0145445.

Le Hello, S., Hendriksen, R. S., Doublet, B., Fisher, I., Nielsen, E. M., Whichard, J. M., ... & Weill, F. X. (2011). International spread of an epidemic population of Salmonella enterica serotype Kentucky ST198 resistant to ciprofloxacin. Journal of Infectious Diseases, 204(5), 675-684.

Mermin JH, Villar R, Carpenter J, Roberts L, Samaridden A, Gasanova L 1999: A massive epidemic of multidrug-resistant typhoid fever in Tajikistan associated with consumption of municipal water. The Journal of infectious diseases 179(6): 1416-1422.

Nair, V. T. D., Venkitanarayanan, K., & Kollanoo, J. A. (2018). Antibiotic-Resistant Salmonella in the Food Supply and the Potential Role of Antibiotic Alternatives for Control. Foods, 7, Article No. 167. https://doi.org/10.3390/foods7100167

Ochiai, R. L., Acosta, C. J., Danovaro-Holliday, M. C., Baiqing, D., Bhattacharya, S. K., Agtini, M. D., ... & Clemens, J. D. (2008). A study of typhoid fever in five Asian countries: disease burden and implications for controls. Bulletin of the World Health Organization, 86, 260-268.

Parry CM, Hoa NTT, Diep TS, Wain J, Chinh NT, Vinh H 1999: Value of a single-tube Widal test in diagnosis of typhoid fever in Vietnam. Journal of Clinical Microbiology 37(9): 2882-2886.

Parry, C. M., Hien, T. T., Dougan, G., White, N. J., & Farrar, J. J. (2002). Typhoid fever. New England Journal of Medicine, 347(22), 1770-1782.

Rahman, M., Siddique, A. K., Tam, F. C., & Nishibuchi, M. (1997). Emergence of multidrug-resistant Salmonella Typhi in Bangladesh. Journal of Medical Microbiology, 46(8), 579-581.

Rowe, B., Ward, L. R., & Threlfall, E. J. (1997). Multidrug-resistant Salmonella typhi: a worldwide epidemic. Clinical Infectious Diseases, 24(Suppl 1), S106-S109.

Saha, S. K., Baqui, A. H., Hanif, M., Darmstadt, G. L., Ruhulamin, M., & Nagatake, T. (2001). Typhoid fever in Bangladesh: Implications for vaccination policy. Pediatric Infectious Disease Journal, 20(5), 521-524.

Saha, S. K., Talukder, S. Y., Islam, M., & Saha, S. (1999). A highly ceftriaxone-resistant Salmonella typhi in Bangladesh. Pediatric Infectious Disease Journal, 18(4), 387-388.

Sur, D., Ali, M., von Seidlein, L., Manna, B., Deen, J. L., & Clemens, J. D. (2007). Comparative immunogenicity of two Salmonella enterica serovar Typhi vaccines in children aged 2 to 5 years in Kolkata, India. Clinical and Vaccine Immunology, 14(7), 888-893.

Tasnim, T., Tarafder, S., Alam, F., Sattar, H., & Mostofa, K. S. (2018). Pre-Extensively Drug Resistant Tuberculosis (Pre-XDR-TB) among Pulmonary Multidrug Resistant Tuberculosis (MDR-TB) Patients in Bangladesh. Journal of Tuberculosis Research, 6, 199-206. https://doi.org/10.4236/jtr.2018.63018

Wain, J., Bay, P. V., Vinh, H., Duong, N. M., Diep, T. S., Walsh, A. L., ... & White, N. J. (1999). Quantitation of bacteria in bone marrow from patients with typhoid fever: relationship between counts and clinical features. Journal of Clinical Microbiology, 37(5), 1571-1576.

Walid, E., Abdelhalim, M. H., Wael, F. E. T., & Asmaa, S. (2017). Extremely Drug Resistant Salmonella in Broiler Production Chain in Egypt. Life Science Journal, 14, 81-87.

World Health Organization. (2007). Typhoid fever. Retrieved from WHO.

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