Angiogenesis, Inflammation & Therapeutics | Online ISSN  2207-872X
RESEARCH ARTICLE   (Open Access)

Immunomodulatory Effect of Tinospora cordifolia with Special Reference to Suppression of Cytokine Storm Induced in SARS-CoV-2

Aishath Thahuseen Waheed1, Thurga Ayavoo2,3, Karthikeyan Murugesan2,3,  Fouad Saleih R. AL-Suede4,5, Ashok Gnanasekaran2,3*

+ Author Affiliations

Journal of Angiotherapy 5(1) 218-225 https://doi.org/10.25163/angiotherapy.51212522101021

Submitted: 22 August 2021  Revised: 29 October 2021  Published: 10 November 2021 

Abstract

Background: Tinospora cordifolia (T. cordifolia) is one such plant that has been studied for its many medicinal properties. Objective: The objective of this study was to investigate the in vitro exposure of human peripheral blood mononuclear cells to T. cordifolia plant might stimulate the induction of anti-inflammatory cytokines, interleukin-10 and interleukin-37. Methods: T. cordifolia plant powder was sterilized by several methods to eliminate presence of microorganisms in plant powder. The sterilized T. cordifolia plant powder was exposed to peripheral blood mononuclear cells to determine the inhibitory concentration by conducting a cytotoxicity test. ELISA test was performed to check whether T. cordifolia stimulates the peripheral blood mononuclear cells into producing anti-inflammatory cytokines. Results: Pasteurization technique was a success as there was no bacterial or fungal growth observed on nutrient agar, blood agar and sabouraud dextrose agar. The optimal inhibitory concentration of T. cordifolia is 72 mg/ml. The results of the ELISA tests showed the production of interleukin-10 and interleukin-37 when stimulated by T. cordifolia. Conclusion: T. cordifolia plant powder could be a potential alternative for non-steroidal anti-inflammatory drugs as the plant induces secretion of interleukin-10 and interleukin-37 that subsidizes the interleukin-6.

Keywords: Cytokine storm, ELISA, Interleukin-10, Interleukin-37, Tinospora cordifolia

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