Anti-Cancer Activity of Allium Ascalonicum Ethanol Extract Against Human Hepatocellular Carcinoma Cell Line (HepG-2)

Geetha N B ^1*, Chintala Venkatesh ¹, Suresh M ¹, Manikandan S ²

Allium ascalonicum might be a safer, more effective alternative to toxic chemotherapy for combating cancer with targeted potency.

Abstract

Chemotherapy is a widely used medicine for the treatment of cancer globally. However, these agents are highly toxic and very little effective in cancer. Determining the anti-cancer medicine from plant sources with selective toxicity and high potency is essential. In this study, we evaluated the anti-cancer potential of ethanol extract of Allium ascalonicum. Solvent-solvent extraction methods were used to collect the extract from plant. The anti-cancer study was carried out in HepG-2 cells. The results showed the anti-cancer potential of Allium ascalonicum ethanol extract on HepG-2 cells, determining dose-dependent apoptosis and selective cytotoxicity. The extract might modulate the mitochondrial membrane potential and ROS levels to induce apoptosis of cancer cells. In conclusion, Allium ascalonicum might be useful for treating Hepatocellular Carcinoma.

Keywords: HepG-2 cells, Vero cells, MTT assay, DAPI staining,  DCFDA staining.

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