In Vitro Anti-Inflammatory Potential of Mangifera Indica Peels

Bhaskaran Sathyapriya¹, Jayesh S Raghavendra², Swamikannu Bhuminathan³, Adugula Chandrakala⁴, Kesavaram Padmavathy^5*, Bharathwaj D K⁶, Indhumathi Krishnaswamy⁶

This study determined Mangifera indica peels' significant anti-inflammatory effects, supporting their use in natural remedies and sustainable practices.

Abstract

Background: Mangifera indica including the peels, are valued for their therapeutic applications, including anti-inflammatory effects. Previous studies have highlighted the rich bioactive profile of Mangifera indica, including polyphenols, flavonoids, and tannins, which contribute to its diverse pharmacological activities. Methods: Fresh mango peels were collected, dried, and powdered. The peel powder was extracted using 80% acetone, and the extract was concentrated and stored for analysis. The anti-inflammatory activity was assessed via a protein denaturation inhibition assay, involving a reaction mixture of bovine serum albumin (BSA) with varying concentrations of the peel extract. The assay measured the extent of protein denaturation induced by heat, with the percentage inhibition calculated spectrophotometrically at 660 nm. Aspirin was used as a standard for comparison. Results: Mangifera indica peel extracts exhibited significant anti-inflammatory activity, with inhibition of protein denaturation increasing in a dose-dependent manner. At a concentration of 500 µg/mL, the extract achieved a maximum inhibition of 71.93 ± 1.12%. The IC50 value was determined to be 119.35 ± 1.99 µg/mL. Aspirin, at 200 µg/mL, showed a maximum inhibition of 77.12 ± 1.42%. These results suggest that the anti-inflammatory efficacy of Mangifera indica peel extracts is comparable to that of aspirin. Conclusion: This study demonstrates that Mangifera indica peels possess significant anti-inflammatory activity, attributed to their polyphenol content and ability to inhibit protein denaturation.

Keywords: Mangifera indica, Anti-inflammatory, Protein denaturation, Peel extracts, Bioactive compounds

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