Ethosomal formulation composed of standardized Orthosiphon stamineus extract and sophorolipid inhibits angiogenesis of melanoma in vivo
Mansoureh Nazari Vishkaei a, Mohamed Khadeer Ahamed Basheer c, Amin Malik Shah Abdul Majid a,b
Journal of Angiotherapy 2(1) 087-096 https://doi.org/10.25163/angiotherapy.1120591141830219
Submitted: 17 January 2019 Revised: 03 February 2019 Published: 04 February 2019
SLOS vesicles can be considered for effective dermal therapeutic agent for the treatment of skin cancer.
Abstract
Cancer disease involves the abnormal growth of cells that have the ability to invade other organs. Melanoma is one of the angiogenesis dependent cancer. Orthosiphon stamineus (EXOS) extract is a well-known anti-angiogenic herb in South Asia. Thin-film hydration method has been employed to develop ethosome, a flexible liposomal gel formulation composed of standard extract of O.S leaves (SLOS) using sophorolipid (SL) and investigated its physicochemical properties for topical drug delivery system. High Performance Liquid Chromatography (HPLC) determined 76.02.02±1.2% entrapment of rosmarinic acid (RA) in ethosome vesicle. The average particle size of optimized vesicle was 189.9 nm with potential of -35.5 mV. SLOS at concentration of 1% demonstrated proper plastic flow, which could facilitate achieving effective and maximum skin permeation of EXOS. Permeation studies using albino mice revealed that the SLOS demonstrated higher penetrability through deeper layers of skin than the conventional hydroethanolic gel composed of EXOS. Cytotoxic test on normal endothelial cells confirmed the safety of SLOS formulation. In vivo melanoma tumor model study revealed that SLOS showed 97.07±0.6% antitumor activity during 16 days of study. These results revealed that SLOS vesicles can be considered for effective dermal therapeutic agent for the treatment of skin cancer.
Key words: Ethosome; sophorolipid; Orthosiphon stamineus; Melanoma; Rheology; Topical drug delivery
References
Bragagni, M., Scozzafava, A., Mastrolorenzo, A., Supuran, C. T., & Mura, P. (2015). Development and ex vivo evaluation of 5-aminolevulinic acid-loaded niosomal formulations for topical photodynamic therapy. International Journal of Pharmaceutics, 494(1), 258-263.
Hossain, M. A., & Rahman, S. M. (2015). Isolation and characterisation of flavonoids from the leaves of medicinal plant Orthosiphon stamineus. Arabian Journal of Chemistry, 8(2), 218-221.
Iizhar, S. A., Syed, I. A., Satar, R., & Ansari, S. A. (2016). In vitro assessment of pharmaceutical potential of ethosomes entrapped with terbinafine hydrochloride. Journal of advanced research, 7(3), 453-461.
Jain, S., Patel, N., Madan, P., & Lin, S. (2016). Formulation and rheological evaluation of ethosome-loaded carbopol hydrogel for transdermal application. Drug development and industrial pharmacy, 42(8), 1315-1324.
Li, K., Gao, S., Tian, B., Shi, Y., Lv, Q., & Han, J. (2018). Formulation Optimization and In-vitro and In-vivo Evaluation of Lornoxicam Ethosomal Gels with Penetration Enhancers. Current drug delivery, 15(3), 424-435.
Malla, B., Malla, K., Sah, A. K., Koirala, A., Karki, S., Prajuli, D. R., & Thapa, B. (2016). Preparation and Characterization of Liposomes and Ethosomes Bearing Indomethacin for Topical drug delivery.
Mbah, C. C., Builders, P. F., Agubata, C. O., & Attama, A. A. (2017). Development of ethosomal vesicular carrier for topical application of griseofulvin: effect of ethanol concentration. Journal of Pharmaceutical Investigation, 1-10.
Ng, K.-W., Salhimi, S. M., Majid, A. M. S. A., & Chan, K.-L. (2010). Anti-angiogenic and cytotoxicity studies of some medicinal plants. Planta medica, 76(09), 935-940.
Ramadon, D., Goldie, A. W., & Anwar, E. (2017). Novel transdermal ethosomal gel containing green tea (Camellia sinensis L. Kuntze) leaves extract: formulation and in vitro penetration study. Journal of Young Pharmacists, 9(3), 336.
Rezaei-Sadabady, R., Eidi, A., Zarghami, N., & Barzegar, A. (2016). Intracellular ROS protection efficiency and free radical-scavenging activity of quercetin and quercetin-encapsulated liposomes. Artificial cells, nanomedicine, and biotechnology, 44(1), 128-134.
Shafaei, A., Ab Halim, N. H., Zakaria, N., & Ismail, Z. (2017). Analysis of free amino acids in different extracts of Orthosiphon stamineus leaves by high-performance liquid chromatography combined with solid-phase extraction. Pharmacognosy magazine, 13(Suppl 3), S385.
Shafaei, A., Sultan Khan, M. S., Aisha, A. F., Abdul Majid, A. M. S., Hamdan, M. R., Mordi, M. N., & Ismail, Z. (2016). Flavonoids-rich Orthosiphon stamineus extract as new candidate for angiotensin I-converting enzyme inhibition: A molecular docking study. Molecules, 21(11), 1500.
Vishkaei, M. N., Sultan, S. B. S. H., Basheer, M. K. A., & Majid, A. M. (2017). SKIN BARRIER CHALLENGES IN DERMAL AND TRANSDERMAL DRUG DELIVERY SYSTEMS.
Vishkaei, M. N., Sultan, S. B. S. H., Khadeer, M. B., & Ahamed, A. M. (2017). World Journal of Pharmaceutical Sciences. Regulation, 15, 7.
View Dimensions
View Altmetric
Save
Citation
View
Share