Antioxidant Potential of Curculigo pilosa Rhizome Extracts in Hydrogen Peroxide Induced Drosophila melanogaster In Vivo
Damilola A. Omoboyowa1*, Elijah A. Agboola 1, Damilola S. Bodun 1, Simbo T. Akinsulure 1, Samia Belahcene 2, Dhurgham Al-Fahad 3
Journal of Angiotherapy 9(1) 1-8 https://doi.org/10.25163/angiotherapy.9110134
Submitted: 04 November 2024 Revised: 09 January 2025 Published: 10 January 2025
Abstract
Background: The scavenging of oxidants and the inhibition of their conversion into harmful metabolites are essential for maintaining the health of living organisms. As a result, there has been significant interest in identifying natural antioxidants. This study investigates the antioxidant potential of Curculigo pilosa rhizome extracts in mitigating oxidative stress in Drosophila melanogaster (Harwich strain) induced with hydrogen peroxide (H2O2). Methods: The radical scavenging activity of butanol (BECP), methanol (MECP), and ethyl acetate (EECP) extracts of C. pilosa was evaluated using standard assays, including 1,1-diphenyl-2-picrylhydrazyl (DPPH), lipid peroxidation (LPO), nitric oxide (NO) inhibition, and ferric reducing antioxidant power (FRAP). The most potent extracts (MECP and BECP) were further assessed in vivo using H2O2-induced Drosophila. Molecular docking studies were conducted using the Schrödinger suite to evaluate the interaction between BECP-derived compounds, identified via HPLC, and Drosophila glutathione-S-transferase (GST). Results: MECP and BECP demonstrated significant antioxidant activity, comparable to butylated hydroxytoluene (BHT), in DPPH, LPO, NO inhibition, and FRAP assays. In vivo, these extracts enhanced the survival rate of Drosophila at concentrations of 2 mg and 4 mg/10 g diet. Flies treated with ascorbic acid, MECP, and BECP exhibited significantly increased (P < 0.05) negative geotaxis performance and reduced NO levels compared to H2O2-induced flies on a normal diet. Additionally, MECP and BECP significantly (P < 0.05) upregulated mRNA expression of GST and glutathione peroxidase (GPx) in H2O2-induced flies. Molecular docking revealed that phlorizin and curculigoside had high binding affinities to Drosophila GST (-6.65 kcal/mol and -6.54 kcal/mol, respectively), comparable to glutathione (-5.97 kcal/mol). Conclusion: The butanol and methanol extracts of Curculigo pilosa rhizome enhanced the free radical scavenging capacity of Drosophila melanogaster and mitigated H2O2-induced oxidative stress, likely due to their bioactive antioxidant compounds. These findings suggest the potential of C. pilosa as a natural source of antioxidants for managing oxidative stress-related conditions.
Keywords: Antioxidant; Free radicals; Natural compounds; Hydrogen peroxide; Drosophila
References
Adebayo SA, Ondua M, Shai LJ, Lebelo SL (2019) Inhibition of nitric oxide production and free radical scavenging activities of four South African medicinal plants. J. Inflamm. Res. 12: 195-204
Adedara AO, Babalola AD, Stephano F, Awogbindin IO, Olopade JO, Rocha JBT, Whitworth AJ, Abolaji AO (2022) An assessment of the rescue action of resveratrol in parkin loss of function -induced oxidative stress in Drosophila melanogaster. Sci. Rep. 12: 3922–3934
Adefegha SA, Oyeleye SI, Oboh G (2018) African crocus (Curculigo pilosa) and wonderful kola (Buchholzia coriacea) seeds modulate critical enzymes relevant to erectile dysfunction and oxidative stress. J. Complemen. Integrat. Med. 15: 1-12
Anadozie SO, Asueiza UA, Olusola BA (2023) Alkaloid-rich extract of Buchholzia coriacea seed mitigate the effect of copper-induced toxicity in Drosophila melanogaster. Vegetos, (https://doi.org/10.1007/s42535-023-00760-9
Arika W, Kibiti CM, Njagi JM, Ngugi MP (2019) In Vitro Antioxidant Properties of Dichloromethanolic Leaf Extract of Gnidia glauca (Fresen) as a Promising Antiobesity Drug. Journal of Evidence-Based Integrative Medicine, 24: 1-17
Averill-Bates DA (2023) Chapter 5: The antioxidant glutathione. Vitamins and hormones. G Litwack Acad Press 121: 109–141
Belahcene S, Widad K, Omoboyowa DA, Alshihri AA, Alelyani M, Bakkour Y, Leghouchi EJ (2023) Unveiling the Chemical Profiling Antioxidant and Anti-Inflammatory Activities of Algerian Myrtus communis L. Essential Oils, and Exploring Molecular Docking to Predict the Inhibitory Compounds against Cyclooxygenase-2. Pharmaceuticals, 16: 1343.
Balogun, T.A., Iqbal, M.N., Saibu, O.A., Akintubosun, M.O., Lateef, O.M., Nneka, U.C., Abdullateef, O.T., Omoboyowa, D.A., 2021. Discovery of potential HER2 inhibitors from Mangifera indica for the treatment of HER2-positive breast cancer: an integrated computational approach. J. Biomol. Struct. Dyn. 39, 1–12.
Chattopadhyay D, James J, Roy D, Sen S, Chatterjee R, Thirumurugan K (2015) Effect of semolina-jaggery diet on survival and development of Drosophila melanogaster, Fly 9: 16–21
Chaudhary P, Janmeda P, Docea AO, Yeskaliyeva B, Abdull-Razis AF, Modu B, Calina D, Sharifi-Rad J (2023) Oxidative stress, free radicals and antioxidants: potential crosstalk in the pathophysiology of human diseases. Front. Chem. 11: 1158198.
Ebrahimzadeh MA, Nabavi1 SF, Nabavi1 SM, Pourmorad F (2010) Nitric oxide radical scavenging potential of some Elburz medicinal plants. Afri. J. Biotechnol. 9: 5212-5217
Ellman GL (1959) Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82: 70–77
Fatoki JO, Kehinde SA, Adegoke AA, Faokunla O, Atere TG, Olajide AT (2022) Inhibition of lipid peroxidation and free radical scavenging activities of methanolic leaf extract of Psidium guajava. Res. J. of Health Sci. 10: 139-150
Gbadamosi IT, Egunyomi A (2010) Phytochemical screening and invitro anti-candidal activity of extracts and essential oil of Curculigo pilosa (Schum and Thonn) Engl. Hypoxidaceae, Afr. J. Biotechnol. 9: 1236–1240
Hachiya M, Akashi M (2005) Catalase regulates cell growth in HL60 human promye-locytic cells: evidence for growth regulation by H2O2, Radiat. Res. 163: 271–282
Hogberg J, Larsen ER, Kristogerson A, Ovrhenius S (1974) NADPH-dependent reductase solubilised from microsomes of peroxidation and its activity. Biochem. and Biophys. Res. Commu. 56: 836.
Jiménez-Estrada M, Velázquez-Contreras C, Garibay-Escobar A, Sierras-Canchola D, Lapizco-Vázquez R, Ortiz-Sandoval C, Burgos-Hernández A, Robles-Zepeda RE (2013) In vitro antioxidant and antiproliferative activities of plants of the ethnopharmacopeia from northwest of Mexico. BMC Complementary and Alternative Medicine 13: 12-20
Johnmark N, Kinyi HW (2021) Amaranth leaf extract protects against hydrogen peroxide induced oxidative stress in Drosophila melanogaster , BMC Res. Notes, 14: 188–193 .
Karigidi KO, Akintimehin ES, Omoboyowa DA, Adetuyi FO, Olaiya CO (2020) Effect of Curculigo pilosa supplemented diet on blood sugar, lipid metabolism, hepatic oxidative stress and carbohydrate metabolism enzymes in streptozotocin-induced diabetic rats. J. Diabetes Metab. Disord. 19: 1173-1184
Karigidi KO, Olaiya CO (2021) Effects of Curculigo pilosa supplementation on antioxidant and antidiabetic activities of yam ?our. J. Food Sci. Technol. 58: 4110–4117
Lang Y, Ningxuan G, Zhihuan Z, Xianjun M, Yang L, Shufang Y, Yiyun Y, Zhufeng J, Bin L (2024) Classification and antioxidant assays of polyphenols: a review, Journal of Future Foods 4: 193-204
Lee SE, Ju EM, Kim JH (2001) Free radical scavenging and antioxidant enzyme fortifying activities of extracts from Smilax china root. Experimental and Molecular Medicine, 33: 263-268
Men TT, Khang DT, Tuan NT, Trang DTX (2022) Anti-aging effects of Lasia spinosa L. stem extract on Drosophila melanogaster. Food Sci. Technol, Campinas 42: e38721
Mohideen K, Krithika C, Saranya R, Chandini R, Snehashish G, Safal D (2023) Assessment of Oxidative Stress by the Estimation of Lipid Peroxidation Marker Malondialdehyde (MDA) in Patients with Chronic Periodontitis: A Systematic Review and Meta-Analysis, International Journal of Dentistry. 23: 6014706.
Moron MS, Depierre JW, Mannervik B (1979) Levels of glutathione, glutathione reductase and glutathione S-transferase activities in rat lung and liver. Biochim. Biophys. Acta. 582: 67–78
Njoya EM, Munvera AM, Mkounga P, Nkengfack AE, McGaw LJ (2017) Phytochemical analysis with free radical scavenging, nitric oxide inhibition and antiproliferative activity of Sarcocephalus pobeguinii extracts, BMC Complementary and Alternative Medicine 17: 199-208
Omoboyowa DA, Agoi MD, Shodehinde SA, Saibu OA, Saliu JA (2023) Antidiabetes study of Spondias mombin (Linn) stem bark fractions in high-sucrose diet-induced diabetes in Drosophila melanogaster. Journal of Taibah University Medical Sciences 18: 663e675
Omoboyowa DA, Karigidi KO, Aribigbola TC (2020) Bridelia ferruginea Benth leaves attenuates diabetes nephropathy in STZ-induced rats via targeting NGAL/KIM-1/cystatin c gene. Clin. Phytosci. 6: 63-73
Omoboyowa DA, Olugbenga ST, Adetuyi FD, Akinsulure ST, Akinwande KM, Iwuji CB, Ayekolu SF (2022a) Modulatory effects of selected compounds on oxidative stress in hydrogen peroxide-induced Drosophila melanogaster. Pharmacol. Res.-Modern Chin. Med. 5: 100169
Omoboyowa DA, Singh G, Fatoki JO, Oyeneyin OE (2022b) Computational investigation of phytochemicals from Abrus precatorius seeds as modulators of peroxisome proliferator-activated receptor gamma (PPAR ??), J. Biomol. Struct. and Dyn. 35: 1–5
Oyibo, A., Abolaji, A. O., Omoboyowa, D. A., Odunola, O.A. (2023). Vitellaria paradoxa inhibits arsenic-induced toxicity in Drosophila melanogaster via the augmentation of antioxidant system. Toxicology 494 (2023) 153590
Phaniendra A, Jestadi DB, Periyasamy L (2015) Free radicals: Properties, sources, targets, and their implication in various diseases. Indian J. Clin. Biochem. 30: 11–26.
Poodineh J, Nakhaee A (2019) In Vitro Antioxidant and Anti-Lipid Peroxidation Activities of Hydroalcoholic Extracts of Caralluma tuberculata, Root and Aerial Parts. Jundishapur J Nat Pharm Prod. 14: e69685.
Pulido R, Bravo L, Sauro-Calixto F (2000) Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. Journal of Agricultural and Food Chemistry, 48: 3396–3402
Ruberto G, Baratta MT (2000) Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69: 167–174.
Ruch RJ, Cheng SJ, Klaunig JE (1989) Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogen 10: 1003–1008.
Shabir S, Yousuf S, Singh SK, Vamanu E, Singh MP (2022) Ethnopharmacological Effects of Urtica dioica, Matricaria chamomilla, and Murraya koenigii on Rotenone-Exposed D. melanogaster: An Attenuation of Cellular, Biochemical, and Organismal Markers. Antioxidants. 11: 1623
Sreejayan N, Rao MNA (1997) Nitric oxide scavenging by curcuminoids. J. of Pharm. and Pharm. 49: 105–107.
Subedi RP, Vartak RR, Kale PG, (2017) Management of stress exerted by hydrogen per-oxide in Drosophila melanogaster using Abhrak bhasma, J. Appl. Pharm. Sci. 7: 65–71
Ugbaja RN, Akinhanmi TF, James AS, Ugwor EI, Babalola AA, Ezenandu EO, Ugbaja VC, Emmanuel EA (2021) Flavonoid-rich fractions from Clerodendrum volubile and Vernonia amygdalina extenuates arsenic-invoked hepatorenal toxicity via augmentation of the antioxidant system in rats. Clinical Nutrition Open Science 35: 12e25
Wided K, Rouibah H, Lahouel M (2015) Quercetin Protects Liver Cells and Mitochondria Against Doxorubicin Induced Oxidative Stress in Albinos' Rats, Journal of Biologically Active Products from Nature 5: 331-38.
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