Angiogenesis, Inflammation & Therapeutics | Online ISSN  2207-872X
RESEARCH ARTICLE   (Open Access)

Characterization of Essential Oil Composition of Syzygium aromaticum Linn. (Clove) by GC-MS and Evaluation of its Antioxidant Activity

Javed Ahamad 1*

+ Author Affiliations

Journal of Angiotherapy 7(1) 1-5 https://doi.org/10.25163/angiotherapy.719358

Submitted: 15 October 2023  Revised: 26 November 2023  Published: 27 November 2023 

Abstract

Background: Syzygium aromaticum Linn. (Clove, Family: Myrtaceae) is traditionally used as a spice and condiment; and medicinally used as a dental analgesic, carminative, and antiseptic. The Clove buds are widely used as a spice in Kurdish foods and are abundantly available in the local market; the quality of Clove available in the local market of Erbil has not been studied till now. Therefore, the aim of current research is to characterize essential oil composition by gas-chromatography mass spectroscopy (GC-MS) and evaluate its antioxidant potential by the DPPH method. Results: The GC-MS analysis of Clove essential oil resulted identification of 37 chemical compounds which constitute about 99.49% of total essential oil. Clove essential oil was found rich in eugenol (59.87%), caryophyllene (23.58%), α-selinene (4.67%), α-terpinyl acetate (4.12%), and humulene (3.74%). The Clove essential oil was found potent antioxidant with a maximum inhibition of 90.94% and it was found comparable with standard antioxidant compounds such as ascorbic acid (92.94%), and gallic acid (87.80%) inhibition. Conclusion: The present study explores the essential oil composition of Clove found in the Kurdistan region and results also show its essential oil has potent antioxidant activity.

Keywords:  Syzygium aromaticum, Clove, Myrtaceae, Kurdistan, GC-MS, DPPH, Antioxidant.

References

Adam R.P. (2007). Identification of essential oil components by gas chromatography/mass spectroscopy, 4th edition, Allured Publishing Corporation, Carol Stream, Illinois.

Ahamad J, Uthirapathy S, Mohammed Ameen MS, Anwer ET. (2019). Essential oil composition and antidiabetic, anticancer activity of Rosmarinus officinalis L. leaves from Erbil (Iraq). J. Essent. Oil-Bear. Plants, 22(6):1544-53.

Ahamad, J., & Uthirapathy, S. (2021). GC/MS profile and in-vitro α-glucosidase inhibitory activity of essential oil of Eucalyptus camaldulensis Dehnh collected from (Erbil) Iraq. Curr. Bioact. Compd. 17(5), 47-52.

Ahamad, J., Uthirapathy, S., Ameen, M. S., Anwer, E. T., Hussain, F. H., & Mir, S. R. (2020). Chemical composition and in vitro antidiabetic effects of Olea europaea Linn. (Olive). Curr. Bioact. Compd.16(8), 1157-1163.

Ali, M. (2001). Techniques in terpenoid identification, Birla Publication, Delhi, India.

Batiha, G. E. S., Alkazmi, L. M., Wasef, L. G., Beshbishy, A. M., Nadwa, E. H., & Rashwan, E. K. (2020). Syzygium aromaticum L. (Myrtaceae): traditional uses, bioactive chemical constituents, pharmacological and toxicological activities. Biomolecules, 10(2).

Biernasiuk, A., Baj, T., & Malm, A. (2022). Clove essential oil and its main constituent, eugenol, as potential natural antifungals against Candida spp. alone or in combination with other antimycotics due to synergistic interactions. Molecules 28(1), 215.

Chaieb, K., Hajlaoui, H., Zmantar, T., Kahla-Nakbi, A. B., Rouabhia, M., Mahdouani, K., & Bakhrouf, A. (2007). The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzigium aromaticum L. Myrtaceae): a short review. Phytother. Res., 21(6), 501-506.

Chandra Manivannan, A., Malaisamy, A., Eswaran, M., Meyyazhagan, A., Arumugam, V. A., Rengasamy, K. R., & Liu, W. C. (2022). Evaluation of clove phytochemicals as potential antiviral drug candidates targeting SARS-CoV-2 main protease: Computational Docking, molecular dynamics simulation, and pharmacokinetic profiling. Front. Mol. Biosci. 9, 918101.

Cortés-Rojas, D. F., de Souza, C. R. F., & Oliveira, W. P. (2014). Clove (Syzygium aromaticum): a precious spice. Asian Pac. J. Trop. Biomed. 4(2), 90-96.

González-Rivera, J., Duce, C., Falconieri, D., Ferrari, C., Ghezzi, L., Piras, A., & Tine, M. R. (2016). Coaxial microwave assisted hydrodistillation of essential oils from five different herbs (lavender, rosemary, sage, fennel seeds and clove buds): Chemical composition and thermal analysis. Innov. Food Sci. Emerg. Technol., 33, 308-318.

Hamad, K. J., Al-Shaheen, S. J. A., Kaskoos, R. A., Ahamad, J., Jameel, M., & Mir, S. R. (2013). Essential oil composition and antioxidant activity of Lavandula angustifolia from Iraq. Int. Res. J. Pharm, 4(4), 117-120.

Han, X., & Parker, T. L. (2017). Anti-inflammatory activity of clove (Eugenia caryophyllata) essential oil in human dermal fibroblasts. Pharm. Biol., 55(1), 1619-1622.

Haro-González, J. N., Castillo-Herrera, G. A., Martínez-Velázquez, M., & Espinosa-Andrews, H. (2021). Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications, and essential bioactivity for human health. Molecules 26(21), 6387.

Hatami, T., Johner, J. C., Zabot, G. L., & Meireles, M. A. A. (2019). Supercritical fluid extraction assisted by cold pressing from clove buds: Extraction performance, volatile oil composition, and economic evaluation. J. Supercrit. Fluids, 144, 39-47.

Hemalatha, R., Nivetha, P., Mohanapriya, C., Sharmila, G., Muthukumaran, C., & Gopinath, M. (2016). Phytochemical composition, GC-MS analysis, in vitro antioxidant and antibacterial potential of clove flower bud (Eugenia caryophyllus) methanolic extract. Am. J. Food Technol., 53, 1189-1198.

Javed Ahamad, Saima Amin, Kamran J Naquvi, Showkat R Mir. (2023). Gymnemagenin a Promising Drug Candidate for Management of Hyperglycemia: In-Vitro and In-Vivo Study, Journal of Angiotherapy, 7(1), 1-6, 9357

Kaefer, C. M., & Milner, J. A. (2008). The role of herbs and spices in cancer prevention. The J Nutr Biochem., 19(6), 347-361.

Kalemba, D. A. A. K., & Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Curr. Med. Chem., 10(10), 813-829.

Kennouche, A., Benkaci-Ali, F., Scholl, G., & Eppe, G. (2015). Chemical composition and antimicrobial activity of the essential oil of Eugenia caryophyllata cloves extracted by conventional and microwave techniques. J. Biol. Act. Prod. Nat., 5(1), 1-11.

Li, Q., Chang, M., Lai, R., Zhang, H., Song, L., Wang, X., ... & Xu, H. (2023). Potential benefits of spicy food consumption on cardiovascular outcomes in patients with diabetes: A cohort study of the China Kadoorie Biobank. Nutrition, 112, 112062.

Martínez-Tomé, M., Jiménez, A. M., Ruggieri, S., Frega, N., Strabbioli, R., & Murcia, M. A. (2001). Antioxidant properties of Mediterranean spices compared with common food additives. J Food Protec., 64(9), 1412-1419.

Mostafa, A. A. F., Yassin, M. T., Al–Askar, A. A., & Al-Otibi, F. O. (2023). Phytochemical analysis, antiproliferative and antifungal activities of different Syzygium aromaticum solvent extracts. J. King Saud Univ. Sci., 35(1), 102362.

Najibullah, S. N. M., Ahamad, J., Aldahish, A. A., Sultana, S., & Sultana, S. (2021). Chemical characterization and α-glucosidase inhibitory activity of essential oil of Lavandula angustifolia flowers. J. Essent. Oil-Bear. Plants, 24(3), 431-438.

Ofori-Asenso, R., Mohsenpour, M. A., Nouri, M., Faghih, S., Liew, D., & Mazidi, M. (2021). Association of spicy chilli food consumption with cardiovascular and all-cause mortality: a meta-analysis of prospective cohort studies. Angiology, 72(7), 625-632.

Oliveira, G. D. S., Nascimento, S. T., Dos Santos, V. M., & Silva, M. G. (2020). Clove essential oil in the sanitation of fertile eggs. Poultry Sci., 99(11), 5509-5516.

Rosarior, V. L., Lim, P. S., Wong, W. K., Yue, C. S., Yam, H. C., & Tan, S. A. (2021). Antioxidant-rich clove extract, a strong antimicrobial agent against urinary tract infections-causing bacteria in vitro. Trop. Life Sci. Res., 32(2), 45.

Selka MA, Chenafa A and Achouri MY, 2021. Developement and biological activity evaluation of clove essential oil based emulsion. In Annales Pharmaceutiques Francaises. https://doi.org/10.1016/j.pharma.2021.11.006.

Somrani, M., Debbabi, H., & Palop, A. (2022). Antibacterial and antibiofilm activity of essential oil of clove against Listeria monocytogenes and Salmonella enteritidis. Int. J. Food Sci. Technol., 28(4), 331-339.

Van Asten, A. (2002). The importance of GC and GC-MS in perfume analysis. TrAC Trends Anal. Chem., 21(9-10): 698-708.

Zhang, C. D., Hu, X. Y., Wang, H. S., & Yan, F. (2021). GC–MS Analysis of essential oil extracted from Acori tatarinowii Rhizoma: An experiment in Natural product analysis. J. Chem. Educ., 98(9), 3004-3010.

Zhang, L., Gu, B., & Wang, Y. (2021). Clove essential oil confers antioxidant activity and lifespan extension in C. elegans via the DAF-16/FOXO transcription factor. Comp. Biochem. Physiol. Part - C: Toxicol. Pharmacol. 242, 108938.

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