Ancient DNA: Cardiovascular Disease Prediction from Mummies
Anastasia V. Poznyak 1*, Tatyana Vladimirovna Kirichenko 2,5, Dmitry Felixovich Beloyartsev 4, Alexey V. Churov 2,6, Tatiana Ivanovna Kovyanova 1,2, Irina Alexandrovna Starodubtseva 3, Vasily N. Sukhorukov 2,5, and Alexander N. Orekhov 2,5
Journal of Angiotherapy 8(6) 1-9 https://doi.org/10.25163/angiotherapy.869761
Submitted: 29 April 2024 Revised: 23 June 2024 Published: 24 June 2024
Understanding CVD's genetic roots through ancient mummies reveals its historical prevalence and informs contemporary health.
Abstract
The impact of human health disorders on history has been an area of insufficient study, with genetic evidence pivotal in addressing this dilemma. The detection of genetic evidence of pathogens through ancient DNA in mummies has revealed insights into the presence of cardiovascular diseases (CVD) in ancient populations. This review article provides an extensive analysis of studies focused on ancient DNA analysis for predicting CVD from remains of ancient individuals, particularly mummies from various regions across the world. The introduction section explains the multifactorial nature of CVD and the importance of understanding the hereditary susceptibility to this disease. Notably, genome-wide association studies (GWAS) have identified numerous loci related to atherosclerotic (AS) cardiovascular disease, shedding light on the genetic basis of its pathogenesis. The review further discusses the significance of noninvasive procedures, such as computed tomography (CT) scans, in identifying evidence of AS cardiovascular disease in mummified remains. The subsequent sections delve into specific findings from Egyptian, Peruvian, North American, European, and African mummies, each revealing unique insights into the prevalence and characteristics of CVD in ancient populations. A particular focus is placed on the Tyrolean Iceman, whose well-preserved state has enabled comprehensive genetic analyses, leading to the identification of specific single nucleotide polymorphisms associated with CVD risk. In conclusion, the review highlights the potential for future studies to further explore genetic predispositions to CVD in ancient populations, leveraging advancements in ancient DNA analysis techniques and complete genome sequencing. The challenges and opportunities associated with analyzing DNA from mummies, especially in warmer regions, are acknowledged, emphasizing the need for continued research in this fascinating field. Overall, this review emphasizes the potential of ancient DNA analysis in broadening our understanding of the genetic underpinnings of CVD and its prevalence in ancient populations, contributing to both historical and medical knowledge.
Keywords: Cardiovascular Disease (CVD), Genetic predisposition, Ancient DNA (aDNA), Mummified remains, Polygenic risk score (PRS)
References
Abstracts from the 52nd European Society of Human Genetics (ESHG) Conference: Posters. (2019). European Journal of Human Genetics, 27(Suppl 2), 1174–1813. https://doi.org/10.1038/s41431-019-0494-2
Acikgoz, A., & Akkemik, O. (2023). Prevalence and Radiographic Features of Head and Neck Soft Tissue Calcifications on Digital Panoramic Radiographs: A Retrospective Study. Cureus, 15(9), e46025. https://doi.org/10.7759/cureus.46025
Aleyasin, S. A., Navidi, T., & Davoudi, S. (2017). Association between rs10757274 and rs2383206 SNPs as Genetic Risk Factors in Iranian Patients with Coronary Artery Disease. The journal of Tehran Heart Center, 12(3), 114–118.
Allam, A. H., Thompson, R. C., Wann, L. S., Miyamoto, M. I., Nur El-Din, A.el-H., El-Maksoud, G. A., Al-Tohamy Soliman, M., Badr, I., El-Rahman Amer, H. A., Sutherland, M. L., Sutherland, J. D., & Thomas, G. S. (2011). Atherosclerosis in ancient Egyptian mummies: the Horus study. JACC. Cardiovascular imaging, 4(4), 315–327. https://doi.org/10.1016/j.jcmg.2011.02.002
Barbieri, C., Vicente, M., Rocha, J., Mpoloka, S. W., Stoneking, M., & Pakendorf, B. (2013). Ancient substructure in early mtDNA lineages of southern Africa. American journal of human genetics, 92(2), 285–292. https://doi.org/10.1016/j.ajhg.2012.12.010
Binder, M., Roberts, C. A., & Antoine, D. (Eds.). (2023). Evidence from Mummified Tissues. In The Bioarchaeology of Cardiovascular Disease (pp. 39–144). part, Cambridge: Cambridge University Press.
Bodner, M., Iuvaro, A., Strobl, C., Nagl, S., Huber, G., Pelotti, S., Pettener, D., Luiselli, D., & Parson, W. (2015). Helena, the hidden beauty: Resolving the most common West Eurasian mtDNA control region haplotype by massively parallel sequencing an Italian population sample. Forensic science international. Genetics, 15, 21–26. https://doi.org/10.1016/j.fsigen.2014.09.012
Briggs, A. W., Good, J. M., Green, R. E., Krause, J., Maricic, T., Stenzel, U., & Pääbo, S. (2009). Primer extension capture: targeted sequence retrieval from heavily degraded DNA sources. Journal of visualized experiments : JoVE, (31), 1573. https://doi.org/10.3791/1573
Briggs, A. W., Stenzel, U., Meyer, M., Krause, J., Kircher, M., & Pääbo, S. (2010). Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA. Nucleic acids research, 38(6), e87. https://doi.org/10.1093/nar/gkp1163
Briggs, K., Madejski, G., Magill, M., Kastritis, K., de Haan, H. W., McGrath, J. L., & Tabard-Cossa, V. (2018). DNA Translocations through Nanopores under Nanoscale Preconfinement. Nano letters, 18(2), 660–668. https://doi.org/10.1021/acs.nanolett.7b03987
Brotherton, J. M., & Gertig, D. M. (2011). Primary prophylactic human papillomavirus vaccination programs: future perspective on global impact. Expert review of anti-infective therapy, 9(8), 627–639. https://doi.org/10.1586/eri.11.78
Chung, G. E., Park, H. E., Lee, H., Kim, M. J., Choi, S. Y., Yim, J. Y., & Yoon, J. W. (2021). Sarcopenic Obesity Is Significantly Associated With Coronary Artery Calcification. Frontiers in medicine, 8, 651961. https://doi.org/10.3389/fmed.2021.651961
Conde-Valverde, M., Rosa, M., Martínez, I., Marchamalo, J., Pantoja-Pérez, A., Quam, R., Lorenzo, C., Gracia-Téllez, A., García-Fernández, A., Arsuaga, J. L., & Rivera-Rodríguez, T. (2019). A revision of the conductive hearing loss in Cranium 4 from the Middle Pleistocene site of Sima de los Huesos (Burgos, Spain). Journal of human evolution, 135, 102663. https://doi.org/10.1016/j.jhevol.2019.102663
Gignoux, C. R., Henn, B. M., & Mountain, J. L. (2011). Rapid, global demographic expansions after the origins of agriculture. Proceedings of the National Academy of Sciences of the United States of America, 108(15), 6044–6049. https://doi.org/10.1073/pnas.0914274108
González Rojo, P., Pérez Ramírez, C., Gálvez Navas, J. M., Pineda Lancheros, L. E., Rojo Tolosa, S., Ramírez Tortosa, M. D. C., & Jiménez Morales, A. (2022). Vitamin D-Related Single Nucleotide Polymorphisms as Risk Biomarker of Cardiovascular Disease. International journal of molecular sciences, 23(15), 8686. https://doi.org/10.3390/ijms23158686
Green, E. J., & Speller, C. F. (2017). Novel Substrates as Sources of Ancient DNA: Prospects and Hurdles. Genes, 8(7), 180. https://doi.org/10.3390/genes8070180
Green, R. E., Briggs, A. W., Krause, J., Prüfer, K., Burbano, H. A., Siebauer, M., Lachmann, M., & Pääbo, S. (2009). The Neandertal genome and ancient DNA authenticity. The EMBO journal, 28(17), 2494–2502. https://doi.org/10.1038/emboj.2009.222
Gronau, I., Hubisz, M. J., Gulko, B., Danko, C. G., & Siepel, A. (2011). Bayesian inference of ancient human demography from individual genome sequences. Nature genetics, 43(10), 1031–1034. https://doi.org/10.1038/ng.937
Halawa, A. Influence of the traditional food culture of Ancient Egypt on the transition of cuisine and food culture of contemporary Egypt. J. Ethn. Food 10, 11 (2023). https://doi.org/10.1186/s42779-023-00177-4
Huang, Y. X., Wang, S., Gao, Y. Q., Chen, J. H., Wang, X. L., & Li, R. J. (2021). Comparison of mitochondrial genome and development of specific PCR primers for identifying two scuticociliates, Pseudocohnilembus persalinus and Uronema marinum. Parasites & vectors, 14(1), 318. https://doi.org/10.1186/s13071-021-04821-3
Keller, A., Graefen, A., Ball, M. et al. New insights into the Tyrolean Iceman's origin and phenotype as inferred by whole-genome sequencing. Nat Commun 3, 698 (2012). https://doi.org/10.1038/ncomms1701
Keller, A., Graefen, A., Ball, M. et al. New insights into the Tyrolean Iceman's origin and phenotype as inferred by whole-genome sequencing. Nat Commun 3, 698 (2012). https://doi.org/10.1038/ncomms1701
Kessler, T., Vilne, B., & Schunkert, H. (2016). The impact of genome-wide association studies on the pathophysiology and therapy of cardiovascular disease. EMBO molecular medicine, 8(7), 688–701. https://doi.org/10.15252/emmm.201506174
Krause, J., Briggs, A. W., Kircher, M., Maricic, T., Zwyns, N., Derevianko, A., & Pääbo, S. (2010). A complete mtDNA genome of an early modern human from Kostenki, Russia. Current biology : CB, 20(3), 231–236. https://doi.org/10.1016/j.cub.2009.11.068
Kreier F. (2023). Ötzi the Iceman has a new look: balding and dark-skinned. Nature, 10.1038/d41586-023-02562-0. Advance online publication. https://doi.org/10.1038/d41586-023-02562-0
Li, H. Y., Yang, J. F., Chen, M., Xu, L., Wang, W. C., Wang, F., Tong, J. B., & Wang, C. Y. (2008). Visualized regional hypodermic migration channels of interstitial fluid in human beings: are these ancient meridians?. Journal of alternative and complementary medicine (New York, N.Y.), 14(6), 621–628. https://doi.org/10.1089/acm.2007.0606
Lindqvist, C., Schuster, S. C., Sun, Y., Talbot, S. L., Qi, J., Ratan, A., Tomsho, L. P., Kasson, L., Zeyl, E., Aars, J., Miller, W., Ingólfsson, O., Bachmann, L., & Wiig, O. (2010). Complete mitochondrial genome of a Pleistocene jawbone unveils the origin of polar bear. Proceedings of the National Academy of Sciences of the United States of America, 107(11), 5053–5057. https://doi.org/10.1073/pnas.0914266107
Lindqvist, C., Schuster, S. C., Sun, Y., Talbot, S. L., Qi, J., Ratan, A., Tomsho, L. P., Kasson, L., Zeyl, E., Aars, J., Miller, W., Ingólfsson, O., Bachmann, L., & Wiig, O. (2010). Complete mitochondrial genome of a Pleistocene jawbone unveils the origin of polar bear. Proceedings of the National Academy of Sciences of the United States of America, 107(11), 5053–5057. https://doi.org/10.1073/pnas.0914266107
Madaudo, C., Coppola, G., Parlati, A. L. M., & Corrado, E. (2024). Discovering Inflammation in Atherosclerosis: Insights from Pathogenic Pathways to Clinical Practice. International journal of molecular sciences, 25(11), 6016. https://doi.org/10.3390/ijms25116016
Mallah, M. A., Soomro, T., Ali, M., Noreen, S., Khatoon, N., Kafle, A., Feng, F., Wang, W., Naveed, M., & Zhang, Q. (2023). Cigarette smoking and air pollution exposure and their effects on cardiovascular diseases. Frontiers in public health, 11, 967047. https://doi.org/10.3389/fpubh.2023.967047
Nagle, N., van Oven, M., Wilcox, S., van Holst Pellekaan, S., Tyler-Smith, C., Xue, Y., Ballantyne, K. N., Wilcox, L., Papac, L., Cooke, K., van Oorschot, R. A., McAllister, P., Williams, L., Kayser, M., Mitchell, R. J., & Genographic Consortium (2017). Aboriginal Australian mitochondrial genome variation - an increased understanding of population antiquity and diversity. Scientific reports, 7, 43041. https://doi.org/10.1038/srep43041
Nawaz, S. K., Noreen, A., Rani, A., Yousaf, M., & Arshad, M. (2015). Association of the rs10757274 SNP with coronary artery disease in a small group of a Pakistani population. Anatolian journal of cardiology, 15(9), 709–715. https://doi.org/10.5152/akd.2014.5470
Nerlich, A. G., Egarter Vigl, E., Fleckinger, A., Tauber, M., & Peschel, O. (2021). Der Mann aus dem Eis : Lebensszenario und Pathologische Befunde aus 30 Jahren Forschung an der Gletschermumie „Ötzi“ [The Iceman : Life scenarios and pathological findings from 30 years of research on the glacier mummy "Ötzi"]. Der Pathologe, 42(5), 530–539. https://doi.org/10.1007/s00292-021-00961-6
Oras, E., Anderson, J., Tõrv, M., Vahur, S., Rammo, R., Remmer, S., Mölder, M., Malve, M., Saag, L., Saage, R., Teearu-Ojakäär, A., Peets, P., Tambets, K., Metspalu, M., Lees, D. C., Barclay, M. V. L., Hall, M. J. R., Ikram, S., & Piombino-Mascali, D. (2020). Multidisciplinary investigation of two Egyptian child mummies curated at the University of Tartu Art Museum, Estonia (Late/Graeco-Roman Periods). PloS one, 15(1), e0227446. https://doi.org/10.1371/journal.pone.0227446
Owen, K. A., Bell, K. A., Price, A., Bachali, P., Ainsworth, H., Marion, M. C., Howard, T. D., Langefeld, C. D., Shen, N., Yazdany, J., Dall'era, M., Grammer, A. C., & Lipsky, P. E. (2023). Molecular pathways identified from single nucleotide polymorphisms demonstrate mechanistic differences in systemic lupus erythematosus patients of Asian and European ancestry. Scientific reports, 13(1), 5339. https://doi.org/10.1038/s41598-023-32569-6
Pääbo S. (1993). Ancient DNA. Scientific American, 269(5), 86–92. https://doi.org/10.1038/scientificamerican1193-86
Pack, J. D., Xu, M., Wang, G., Baskaran, L., Min, J., & De Man, B. (2022). Cardiac CT blooming artifacts: clinical significance, root causes and potential solutions. Visual computing for industry, biomedicine, and art, 5(1), 29. https://doi.org/10.1186/s42492-022-00125-0
Park, J. S., Son, J. H., Park, C. S., & Chang, H. S. (2019). Clinical Implications of Single Nucleotide Polymorphisms in Diagnosis of Asthma and its Subtypes. Yonsei medical journal, 60(1), 1–9. https://doi.org/10.3349/ymj.2019.60.1.1
Pospieszny, L., & Nowaczyk, S. (2022). Radiocarbon dates and stable isotope data for human and animal skeletal remains from a passage grave in Kierzkowo, Poland (Late Neolithic and modern period). Data in brief, 41, 108011. https://doi.org/10.1016/j.dib.2022.108011
Powell-Wiley, T. M., Poirier, P., Burke, L. E., Després, J. P., Gordon-Larsen, P., Lavie, C. J., Lear, S. A., Ndumele, C. E., Neeland, I. J., Sanders, P., St-Onge, M. P., & American Heart Association Council on Lifestyle and Cardiometabolic Health; Council on Cardiovascular and Stroke Nursing; Council on Clinical Cardiology; Council on Epidemiology and Prevention; and Stroke Council (2021). Obesity and Cardiovascular Disease: A Scientific Statement From the American Heart Association. Circulation, 143(21), e984–e1010. https://doi.org/10.1161/CIR.0000000000000973
Prendergast, M. E., Lipson, M., Sawchuk, E. A., Olalde, I., Ogola, C. A., Rohland, N., Sirak, K. A., Adamski, N., Bernardos, R., Broomandkhoshbacht, N., Callan, K., Culleton, B. J., Eccles, L., Harper, T. K., Lawson, A. M., Mah, M., Oppenheimer, J., Stewardson, K., Zalzala, F., Ambrose, S. H., … Reich, D. (2019). Ancient DNA reveals a multistep spread of the first herders into sub-Saharan Africa. Science (New York, N.Y.), 365(6448), eaaw6275. https://doi.org/10.1126/science.aaw6275
Rawla P, Sharma S. Enterobius Vermicularis. [Updated 2023 Aug 1]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK536974/
Rollo, F., Ubaldi, M., Ermini, L., & Marota, I. (2002). Otzi's last meals: DNA analysis of the intestinal content of the Neolithic glacier mummy from the Alps. Proceedings of the National Academy of Sciences of the United States of America, 99(20), 12594–12599. https://doi.org/10.1073/pnas.192184599
Ruff, C. B., Holt, B. M., Sládek, V., Berner, M., Murphy, W. A., Jr, zur Nedden, D., Seidler, H., & Recheis, W. (2006). Body size, body proportions, and mobility in the Tyrolean "Iceman". Journal of human evolution, 51(1), 91–101. https://doi.org/10.1016/j.jhevol.2006.02.001
Schlebusch, C. M., Sjödin, P., Breton, G., Günther, T., Naidoo, T., Hollfelder, N., Sjöstrand, A. E., Xu, J., Gattepaille, L. M., Vicente, M., Scofield, D. G., Malmström, H., de Jongh, M., Lombard, M., Soodyall, H., & Jakobsson, M. (2020). Khoe-San Genomes Reveal Unique Variation and Confirm the Deepest Population Divergence in Homo sapiens. Molecular biology and evolution, 37(10), 2944–2954. https://doi.org/10.1093/molbev/msaa140
Sharifi-Rad, J., Rodrigues, C. F., Sharopov, F., Docea, A. O., Can Karaca, A., Sharifi-Rad, M., Kahveci Karincaoglu, D., Gülseren, G., Senol, E., Demircan, E., Taheri, Y., Suleria, H. A. R., Özçelik, B., Nur Kasapoglu, K., Gültekin-Özgüven, M., Daskaya-Dikmen, C., Cho, W. C., Martins, N., & Calina, D. (2020). Diet, Lifestyle and Cardiovascular Diseases: Linking Pathophysiology to Cardioprotective Effects of Natural Bioactive Compounds. International journal of environmental research and public health, 17(7), 2326. https://doi.org/10.3390/ijerph17072326
Shimizu, S., Mimura, J., Hasegawa, T., Shimizu, E., Imoto, S., Tsushima, M., Kasai, S., Yamazaki, H., Ushida, Y., Suganuma, H., Tomita, H., Yamamoto, M., Nakaji, S., & Itoh, K. (2020). Association of single nucleotide polymorphisms in the NRF2 promoter with vascular stiffness with aging. PloS one, 15(8), e0236834. https://doi.org/10.1371/journal.pone.0236834
Shin, D. H., Oh, C. S., Hong, J. H., Kim, Y., Lee, S. D., & Lee, E. (2017). Paleogenetic study on the 17th century Korean mummy with atherosclerotic cardiovascular disease. PloS one, 12(8), e0183098. https://doi.org/10.1371/journal.pone.0183098
Skoglund, P., Thompson, J. C., Prendergast, M. E., Mittnik, A., Sirak, K., Hajdinjak, M., Salie, T., Rohland, N., Mallick, S., Peltzer, A., Heinze, A., Olalde, I., Ferry, M., Harney, E., Michel, M., Stewardson, K., Cerezo-Román, J. I., Chiumia, C., Crowther, A., Gomani-Chindebvu, E., … Reich, D. (2017). Reconstructing Prehistoric African Population Structure. Cell, 171(1), 59–71.e21. https://doi.org/10.1016/j.cell.2017.08.049
Thompson, R. C., Allam, A. H., Lombardi, G. P., Wann, L. S., Sutherland, M. L., Sutherland, J. D., Soliman, M. A., Frohlich, B., Mininberg, D. T., Monge, J. M., Vallodolid, C. M., Cox, S. L., Abd el-Maksoud, G., Badr, I., Miyamoto, M. I., el-Halim Nur el-Din, A., Narula, J., Finch, C. E., & Thomas, G. S. (2013). Atherosclerosis across 4000 years of human history: the Horus study of four ancient populations. Lancet (London, England), 381(9873), 1211–1222. https://doi.org/10.1016/S0140-6736(13)60598-X
Thompson, R. C., Sutherland, M. L., Allam, A. H., Paladin, A., Zink, A. R., Wann, L. S., Sutherland, J. D., Frohlich, B., Hunt, D., Miyamoto, M. I., Rowan, C. J., Michalik, D. E., Finch, C. E., Lombardi, G. P., Soliman, M. A., Monge, J. M., Vallodolid, C. M., Cox, S. L., Abdel-Maksoud, G., Badr, I., … Thomas, G. S. (2024). Atherosclerosis in ancient mummified humans: the global HORUS study. European heart journal, ehae283. Advance online publication. https://doi.org/10.1093/eurheartj/ehae283
Thompson, Randall & Mahadev, Ashna & Sutherland, M Linda & Thomas, Gregory. (2023). Computed Tomography Evidence of Atherosclerosis in Ancient Mummies: The Horus Studies of Mummies from Five Continents. 10.1017/9781108648561.006.
Tomei, S., Singh, P., Mathew, R., Mattei, V., Garand, M., Alwakeel, M., Sharif, E., & Al Khodor, S. (2020). The Role of Polymorphisms in Vitamin D-Related Genes in Response to Vitamin D Supplementation. Nutrients, 12(9), 2608. https://doi.org/10.3390/nu12092608
Venter, J. C., Adams, M. D., Myers, E. W., Li, P. W., Mural, R. J., Sutton, G. G., Smith, H. O., Yandell, M., Evans, C. A., Holt, R. A., Gocayne, J. D., Amanatides, P., Ballew, R. M., Huson, D. H., Wortman, J. R., Zhang, Q., Kodira, C. D., Zheng, X. H., Chen, L., Skupski, M., … Zhu, X. (2001). The sequence of the human genome. Science (New York, N.Y.), 291(5507), 1304–1351. https://doi.org/10.1126/science.1058040
Vollenbrock, C. E., Roshandel, D., van der Klauw, M. M., Wolffenbuttel, B. H. R., & Paterson, A. D. (2022). Genome-wide association study identifies novel loci associated with skin autofluorescence in individuals without diabetes. BMC genomics, 23(1), 840. https://doi.org/10.1186/s12864-022-09062-x
Wang, K., Prüfer, K., Krause-Kyora, B., Childebayeva, A., Schuenemann, V. J., Coia, V., Maixner, F., Zink, A., Schiffels, S., & Krause, J. (2023). High-coverage genome of the Tyrolean Iceman reveals unusually high Anatolian farmer ancestry. Cell genomics, 3(9), 100377. https://doi.org/10.1016/j.xgen.2023.100377
Wang, K., Prüfer, K., Krause-Kyora, B., Childebayeva, A., Schuenemann, V. J., Coia, V., Maixner, F., Zink, A., Schiffels, S., & Krause, J. (2023). High-coverage genome of the Tyrolean Iceman reveals unusually high Anatolian farmer ancestry. Cell genomics, 3(9), 100377. https://doi.org/10.1016/j.xgen.2023.100377
Weaver, K. J., Holt, R. A., Henry, E., Lyu, Y., & Pletcher, S. D. (2023). Effects of hunger on neuronal histone modifications slow aging in Drosophila. Science (New York, N.Y.), 380(6645), 625–632. https://doi.org/10.1126/science.ade1662
Weissensteiner, H., Forer, L., Fendt, L., Kheirkhah, A., Salas, A., Kronenberg, F., & Schoenherr, S. (2021). Contamination detection in sequencing studies using the mitochondrial phylogeny. Genome research, 31(2), 309–316. https://doi.org/10.1101/gr.256545.119
Williams, S. T., Foster, P. G., & Littlewood, D. T. (2014). The complete mitochondrial genome of a turbinid vetigastropod from MiSeq Illumina sequencing of genomic DNA and steps towards a resolved gastropod phylogeny. Gene, 533(1), 38–47. https://doi.org/10.1016/j.gene.2013.10.005
Wurst, C., Maixner, F., Paladin, A., Mussauer, A., Valverde, G., Narula, J., Thompson, R., & Zink, A. (2024). Genetic Predisposition of Atherosclerotic Cardiovascular Disease in Ancient Human Remains. Annals of global health, 90(1), 6. https://doi.org/10.5334/aogh.4366
Wurst, C., Maixner, F., Paladin, A., Mussauer, A., Valverde, G., Narula, J., Thompson, R., & Zink, A. (2024). Genetic Predisposition of Atherosclerotic Cardiovascular Disease in Ancient Human Remains. Annals of global health, 90(1), 6. https://doi.org/10.5334/aogh.4366
Xu, L. B., Zhang, Y. Q., Zhang, N. N., Li, B., Weng, J. Y., Li, X. Y., Lu, W. C., Yu, P. R., Wang, X., Li, Y., Han, Z., Chen, L., He, H. T., Zhou, Y. F., Ma, X. X., & Xu, G. D. (2020). Rs10757274 gene polymorphisms in coronary artery disease: A systematic review and a meta-analysis. Medicine, 99(3), e18841. https://doi.org/10.1097/MD.0000000000018841
Yasuda, T., Ishida, T., & Rader, D. J. (2010). Update on the role of endothelial lipase in high-density lipoprotein metabolism, reverse cholesterol transport, and atherosclerosis. Circulation journal : official journal of the Japanese Circulation Society, 74(11), 2263–2270. https://doi.org/10.1253/circj.cj-10-0934
Zaharan, N. L., Muhamad, N. H., Jalaludin, M. Y., Su, T. T., Mohamed, Z., Mohamed, M. N. A., & A Majid, H. (2018). Non-Synonymous Single-Nucleotide Polymorphisms and Physical Activity Interactions on Adiposity Parameters in Malaysian Adolescents. Frontiers in endocrinology, 9, 209. https://doi.org/10.3389/fendo.2018.00209
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