Immunological and Molecular Analysis of Virulence and Resistance of Pseudomonas aeruginosa in Urinary Tract Infections
Immunological and Molecular Analysis of Virulence and Resistance of Pseudomonas aeruginosa in Urinary Tract Infections
Zainab Hayder Jaber Alkufaishi 1, Lamees A. Abdul-Lateef 2 *, Anwar Kadhim Hussein AL-saffar 3, Jwan Ahmed Ali Alhamawandi 4
Journal of Angiotherapy 8(4) 1-6 https://doi.org/10.25163/angiotherapy.849601
Submitted: 06 February 2024 Revised: 02 April 2024 Published: 08 April 2024
Abstract
Background: Pseudomonas aeruginosa is an opportunistic pathogen notorious for its role in antibiotic resistance and nosocomial infections. This study investigates immunological markers in UTI with P. aeruginosa. Method: Between June 2022 and January 2023, 150 patients with suspected UTIs and 40 healthy controls were enrolled at AL-Hilla Teaching Hospital. Molecular tests, including PCR for MexA and pareE genes, and ELISA tests for TNF-α, IL-12, and IL-17, were conducted. Results: Out of 150 samples, 20 (13.3%) were culture-negative, while 90 harbored different bacterial species. Pseudomonas aeruginosa was isolated in 26.6% of samples, with 40% positive for MexA and 70% for pareE genes via PCR. UTI patients with P. aeruginosa showed significantly higher TNF-α (620.53 ± 18.455 ng/ml) and TNF-β levels (630.32 ± 25.150 ng/ml) compared to controls (P <0..01). Conclusion: Elevated levels of IL-12 and IL-17 were observed in P. aeruginosa-infected UTI patients compared to TNF-α levels, highlighting a distinct cytokine profile associated with Pseudomonas aeruginosa UTIs.Key words: Pseudomonas aurogenosa ,TNF, IL-12, IL-17 ,pareE and MexA.
Keywords: Pseudomonas aeruginosa, urinary tract infections, virulence factors, antibiotic resistance, cytokine analysis
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