Angiogenesis, Inflammation & Therapeutics | Impact 0.1 (CiteScore) | Online ISSN  2207-872X
RESEARCH ARTICLE   (Open Access)

A Rapid Diagnosis Development of Canine Enteric Coronavirus Inflammation

Mohammed Ali Hussein 1,2*, Mawlood Abbas Ali Al-Graibawi 1

+ Author Affiliations

Journal of Angiotherapy 8(4) 1-8 https://doi.org/10.25163/angiotherapy.849521

Submitted: 28 January 2024  Revised: 04 April 2024  Published: 07 April 2024 

This study elucidated the prevalence of CECoV in Baghdad, Iraq, highlighting its zoonotic potential and emphasizing the importance of surveillance and diagnostic tools.

Abstract


Background: Canine Enteric Coronavirus (CECoV) is a significant viral pathogen causing gastrointestinal disturbances in dogs worldwide. However, data regarding CECoV in Iraq remains scarce. This study aimed to investigate CECoV IgG antibodies using ELISA and detect CECoV antigens in feces using lateral flow immunochromatography test. Methods: A total of 211 blood and fecal samples were collected from 161 dogs with diarrhea and 50 apparently healthy dogs (control group) in Baghdad, Iraq. Clinical signs were recorded, and samples were analyzed for CECoV IgG antibodies and antigens. Hematological parameters were also assessed. Results and Discussion: Clinical examination revealed gastrointestinal disturbances in 76.3% of dogs, with diverse clinical signs observed. ELISA results showed 21.8% seropositivity for CECoV IgG antibodies, with none detected in apparently healthy dogs. Lateral flow immunochromatography identified CECoV antigens in 7.58% of fecal swabs. Hematological changes included leukopenia, lymphopenia, and increased PCV and MCV values. These findings align with previous studies and suggest the ongoing prevalence and clinical significance of CECoV infection in Iraqi dogs. Conclusion: The combined use of ELISA and lateral flow immunochromatography tests can facilitate the diagnosis of CECoV infection in canine practice. This study provides valuable insights into the epidemiology and clinical impact of CECoV in Iraq, contributing to the global understanding of this viral pathogen.

Keywords: Dog, Canine enteric coronavirus (CECoV), ELISA, immunochromatography, Zoonotic disease, Public health

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