Nanoparticle-Mediated Plasmid Curing in Combating Antibiotic Resistance in Pathogenic Bacteria

Muntaha R. Ibraheem ^1*, Dhafar N. Al-Ugaili ²

Novel approach fights antibiotic resistance. Enhances DNA extraction efficiency. Eliminates resistance plasmids, aiding biotechnology. Highlights research needs for nanoparticle optimization and mechanism understanding.

Abstract

Background: Nanotechnology has emerged as a pivotal domain in material science research with extensive applications across various sectors including biotechnology and medicine. Nanoparticles offer unique properties facilitating advancements in nanobiotechnology, particularly in nanomedicine, to combat bacterial infections and antibiotic resistance. This study aimed to determine the application of nanoparticles, specifically nano-TiO2, in treating plasmid-mediated antibiotic resistance in both Gram-negative and Gram-positive bacteria. Method: We evaluated antibiotic and nanomaterial sensitivity through disc diffusion and broth microdilution assays. Plasmid curing experiments were conducted using varying concentrations of nano-TiO2 and SDS as curing agents, followed by plasmid isolation and DNA extraction. The efficacy of nano-TiO2 in plasmid curing and DNA extraction was assessed, alongside the impact on bacterial growth and antibiotic resistance. Results: Results showed successful plasmid elimination with nano-TiO2 treatment, evidenced by the loss of plasmid DNA bands. Additionally, nano-TiO2 substantially enhanced DNA extraction efficiency and quality. The study indicated nano-TiO2's potential in combating antibiotic resistance by targeting plasmids, thereby presenting a novel approach in molecular biology techniques. Conclusion: In conclusion, this study underscores the promising role of nanoparticles in addressing bacterial infections and combating antibiotic resistance. Nano-TiO2 emerges as a valuable tool in DNA purification and plasmid curing, offering new avenues in molecular biology and antibiotic resistance research. However, further investigations are warranted to elucidate the broader implications of nanoparticles across diverse bacterial species and strains. These findings represent a significant step towards harnessing the potential of nanotechnology in combating antimicrobial resistance and advancing healthcare paradigms.

Keywords: Nanoparticle-mediated plasmid curing, Antibiotic resistance, DNA extraction efficiency, Biotechnological advancements, Mechanisms of nanoparticle action

References

Belaynehea K. M., Seung Won Shin , Kyung Yoon Park , Young Jang , Ho Geun Won , In Joong Yoon , Han Sang Yoo (2018). Emergence of mcr-1 and mcr-3 variants coding for plasmid-mediated colistin resistance in Escherichia coli isolates from food- producing animals in South Korea. International Journal of Infectious Diseases, 22-24.

Daoud Ali, et al. (2015). Cerium Oxide Nanoparticles Induce Oxidative Stress and Genotoxicity in Human Skin Melanoma Cells. Cell Biochem Biophys, 1643–1651.

Debashish A., et al. (2018). Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria. Scientific Reports, 1-11.

Dongshu Wang, et al. (2015). Curing Both Virulent Mega-Plasmids from Bacillus anthracis Wild-Type Strain A16 Simultaneously Using Plasmid Incompatibility. J. Microbiol. Biotechno, 1614–1620.

Dutta, R.K., Bhavani P Nenavathu, Mahesh K Gangishetty, (2012). Studies on antibacterial activity of ZnO nanoparticles by ROS induced lipid peroxidation. Colloids and Surfaces B: Biointerfaces, 143-150.

Ferraro, J. H. (2009). Antimicrobial Susceptibility Testing: A Review of General Principles and Contemporary Practices. MEDICAL MICROBIOLOGY, 1749-1755.

Fidel Martinez-Gutierrez, L. B., et al (2013). Anti-biofilm activity of silver nanoparticles against different microorganisms. Biofouling, 651-660.

Gloria Aderonke Otunola, et al.  (2017). CCharacterization, Antibacterial and Antioxidant Properties of Silver Nanoparticles Synthesized from Aqueous Extracts of Allium sativum, Zingiber officinale, and Capsicum frutescens. Pharmacognosy Magazine, s201-s208.

Haley J. Morrill, Jason M. Pogue, Keith S. Kaye, and Kerry L. LaPlante. (2015). Treatment Options for Carbapenem-Resistant Enterobacteriaceae Infections. open forum infectious disease, 1-15.

Jun Feng, et al. (2013). Curing the plasmid pMC1 from the poly (γ-glutamic acid) producing Bacillus amyloliquefaciens LL3 strain using plasmid incompatibility. Appl Biochem Biotechnology, 532-542.

Klepetsanis Pavlos (2022). Antibacterial Efficacy and Proteomic Response of Silver Nanoparticles in Escherichia coli XL1-Red, Biosensors and nanotheranostics, 1(1), 1-6, 9838.

Linlin W., et al. (2017). The antimicrobial activity of nanoparticles: present situation and prospects for the future. nternational journal of nanomedicin, 1227-1249.

Lowrence Rene Christena, et al. (2015). Copper nanoparticles as an efflux pump inhibitor to tackle drug resistant bacteria. RSC advances, 12899-12909.

Malarkodi,C.,  et al (2013). Novel eco-friendly synthesis of titanium oxide nanoparticles by using Planomicrobium sp. and its antimicrobial evaluation. Der Pharmacia Sinica, 59-66.

Mohammad J Hajipour, K. M. et al.(2012). Antibacterial properties of nanoparticles. Trends in Biotechnology, 499-511.

Muhammad Kamruzzaman, et al. (2017). Plasmid interference for curing antibiotic resistance plasmids in vivo. PLOS ONE, 1-20.

Ningning Song, J. X.-P. (2015). Curing a large endogenous plasmid by single substitution of a partitioning gene. plasmid, 10-16.

Priyanka Padwal,et al. (2014). Polyacrylic acid-coated iron oxide nanoparticles for targeting drug resistance in mycobacteria. RSC Advances, 15266–15276

Rezak Drali , Meryem Berrazeg, Laldja Lilia Zidouni, Fella Hamitouche , Amina Aicha Abbas , Abdelhamid Deriet , Fawzia Mouffok (2018). Emergence of mcr-1 plasmid-mediated colistin-resistant Escherichia coli isolates from seawater. Science of the Total Environment, Pages 90-94.

Robert Lotha, et al. (2018). Plant nutraceuticals (Quercetrin and Afzelin) capped silver nanoparticles exert potent antibiofilm effect against food borne pathogen Salmonella enterica serovar Typhi and curtail planktonic growth in zebrafish infection model. Microbial Pathogenesis, 109-118.

Ruobing Wang, Yu-Qing Liu. (2018). The prevalence of colistin resistance in Escherichia coli and Klebsiella pneumoniae isolated from food animals in China: coexistence of mcr-1 and blaNDM with low fitness cost. International Journal of Antimicrobial Agents, Pages 739-744.

Sengan Megarajan, et al. (2016). N-lauryltyramine capped copper nanoparticles exhibit a selective colorimetric response towards hazardous mercury(ii) ions and display true anti-biofilm and efflux pump inhibitory effects in E. coli. RSC Advances, 87513-87522.

Solmaz Maleki Dizaj, F. L.-J. et al (2014). Antimicrobial activity of the metals and metal oxide nanoparticles. Materials Science and Engineering, Pages 278-284.

Steven C Hayden, et al. (2012). Aggregation and Interaction of Cationic Nanoparticles on Bacterial Surfaces. Journal of American Chemecal Society, 6920–6923.

Sukdeb Pal, Yu Kyung Tak, Joon Myong Song. (2007). Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli. Applied and Environmental Microbiology, 1712 - 1720.

Trevors, J. (1986). Plasmid curing in bacteria. Federation of European Microbiological Societies, pp. 149-157.

Vel  Berzin, Michael Kiriukhin, and Michael Tyurin. (2013). Curing” of plasmid DNA in acetogen using microwave or applying an electric pulse improves cell growth and metabolite production as compared to the plasmid-harboring strain. Archives of Microbiology, pages 181–188.

Warawan Eiamphungporn (2018). Prevalence of the colistin resistance gene mcr-1 in colistin-resistant Escherichia coli and Klebsiella pneumoniae isolated from humans in Thailand. Journal of Global Antimicrobial Resistance, Pages 32-35.

Yi-Yun Liu, et. al. (2016). Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. The Lancet Infectious Diseases, 161-168.