In vitro Cytotoxicity and Apoptotic Activity of Aquous, Chloroform and Methanol Extracts of Kiwi Fruit Actinidia deliciosa in MCF-7 cells

Kartheeswari ¹, Archana C ¹, Anupreyaa K ¹, Saranya M ²

Assessment of A. deliciosa extracts' anticancer potential on MCF-7 cells offers insights for natural cancer medication development

Abstract

The study aimed to assess the anticancer potential of Aquous (AQU), Chloroform (CHL) and Methanol (METH) extract of Actinidia deliciosa on the MCF-7 cell line. MTT cytotoxicity assay was used to determine the anticancer activity of the fruit extracts. DAPI was also used to determine the apoptotic cell imaging. The MTT assays revealed dose-dependent cytotoxic effects, with METH extract exhibiting the highest potency, with an IC50 value of 70.984 μg/ml after 24 hours of incubation. DAPI staining confirmed apoptotic cell death induced by the extract. The observed cytotoxic and anticancer effects are likely attributed to bioactive compounds in A. deliciosa fruit extracts. These findings suggest the potential of A. deliciosa METH extract as a natural source for cancer medication development. Further investigation is needed to identify the active compounds responsible for these effects and their apoptotic pathways. Future research should explore its efficacy in vivo and its potential as a dietary supplement or therapeutic agent.

Keywords: Actinidia deliciosa, Kiwi fruit (KF), MCF-7 cell line, MTT, DAPI staining.

References

Arbyn et al (2020). Estimates of incidence and mortality of cervical cancer in 2018: a worldwide analysis. The Lancet Global Health, 8(2), e191-e203.

Ashok and Sivakumari (2020). Anticancer activity of green synthesized silvernanoparticles. LAP LAMBERT Academic Publishing, Mauritius, pp: 1-178.

Beverland (2001). Creating value through brands: the ZESPRITM kiwi fruit case. British Food Journal.

Chandra, D., & Singh, K. K. (2011). Genetic insights into OXPHOS defect and its role in cancer. Biochimica et Biophysica Acta (BBA)-Bioenergetics, 1807(6), 620-625.

Chengaiah et al (2010). Medicinal importance of natural dyes-a review. International Journal of PharmTech Research, 2(1), 144-154.

Chung and Gillison (2009). Human papillomavirus in head and neck cancer: its role in pathogenesis and clinical implications. Clinical cancer research, 15(22), 6758-6762.

Demark-Wahnefried et al (2012). Weight management and its role in breast cancer rehabilitation. Cancer, 118(S8), 2277-2287.

Drabsch and Ten Dijke (2012). TGF-β signalling and its role in cancer progression and metastasis. Cancer and Metastasis Reviews, 31(3), 553-568.

Engel et al (2014). Pro-apoptotic and anti-adhesive effects of four African plant extracts on the breast cancer cell line MCF-7. BMC complementary and alternative medicine, 14(1), 1-13.

Ferguson and  Ferguson (2002, September). Are kiwifruit really good for you?. In V International Symposium on Kiwifruit 610 (pp. 131-138).

García-Solís et al (2009). Screening of antiproliferative effect of aqueous extracts of plant foods consumed in Mexico on the breast cancer cell line MCF-7. International journal of food sciences and nutrition, 60(sup6), 32-46.

Gerson (2004). MGMT: its role in cancer aetiology and cancer therapeutics. Nature Reviews Cancer, 4(4), 296-307.

Gomathi et al (2020). Anticancer activity of silver nanoparticles synthesized using aqueous fruit shell extract of Tamarindus indica on MCF-7 human breast cancer cell line. Journal of Drug Delivery Science and Technology, 55, 101376.  

Halkidou et al (2003). Expression of Tip60, an androgen receptor coactivator, and its role in prostate cancer development. Oncogene, 22(16), 2466-2477.

Hay (2005). The mesenchymal cell, its role in the embryo, and the remarkable signaling mechanisms that create it. Developmental dynamics: an official publication of the American Association of Anatomists, 233(3), 706-720.

Hunter et al (2011). Antioxidant and ‘natural protective’properties of kiwifruit. Current topics in medicinal chemistry, 11(14), 1811-1820.

Hunter et al (2016). Kiwifruit and health. In Fruits, Vegetables, and Herbs (pp. 239-269). Academic Press.

Jiao et al (2020). hsa_circ_0000745 promotes cervical cancer by increasing cell proliferation, migration, and invasion. Journal of cellular physiology, 235(2), 1287-1295.

Khateef et al (2019). Potential in-vitro anti-breast cancer activity of green-synthesized silver nanoparticles preparation against human MCF-7 cell-lines. Advances in Natural Sciences: Nanoscience and Nanotechnology, 10(4), 045012.

Knight et al (2019). A delicate balance–The BCL-2 family and its role in apoptosis, oncogenesis, and cancer therapeutics. Biochemical pharmacology, 162, 250-261.

Kuhajda  (2000). Fatty-acid synthase and human cancer: new perspectives on its role in tumor biology. Nutrition, 16(3), 202-208.

Latocha et al (2015). Phenolics, ascorbate and the antioxidant potential of kiwiberry vs. common kiwifruit: The effect of cultivar and tissue type. Journal of Functional Foods, 19, 155-163.

Mansoori et al (2014). RNA interference and its role in cancer therapy. Advanced pharmaceutical bulletin, 4(4), 313.

Martin and Jiang (2009). Loss of tight junction barrier function and its role in cancer metastasis. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1788(4), 872-891.

Mesfin et al (2009). An ethnobotanical study of medicinal plants in Wonago Woreda, SNNPR, Ethiopia. Journal of Ethnobiology and Ethnomedicine, 5(1), 1-18.

Moasser  (2007). The oncogene HER2: its signaling and transforming functions and its role in human cancer pathogenesis. Oncogene, 26(45), 6469-6487.

Moore et al (2010). Inflammation and cancer: causes and consequences. Clinical Pharmacology & Therapeutics, 87(4), 504-508.

Moore et al (2010). Inflammation and cancer: causes and consequences. Clinical Pharmacology & Therapeutics, 87(4), 504-508.

Mosmann  (1983). Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65(1-2), 55-63.

Qureshi et al (2015). EMT in cervical cancer: its role in tumour progression and response to therapy. Cancer letters, 356(2), 321-331.

Richardson et al (2018). The nutritional and health attributes of kiwifruit: a review. European journal of nutrition, 57(8), 2659-2676.

Sarli and Ghasemi (2020). Optimization of biosynthesized Zn nanoparticles by poisonous Taxus baccata leaves extract and evaluation of their effect on the bacterias and MCF-7 cancer cells. Eurasian Chemical Communications, 2(3), 302-318.

Singletary  (2012). Kiwifruit: overview of potential health benefits. Nutrition Today, 47(3), 133-147.

Stonehouse et al (2013). Kiwifruit: our daily prescription for health. Canadian journal of physiology and pharmacology, 91(6), 442-447.

Surveswaran et al (2007). Systematic evaluation of natural phenolic antioxidants from 133 Indian medicinal plants. Food chemistry, 102(3), 938-953.

Vilchez and Butel (2004). Emergent human pathogen simian virus 40 and its role in cancer. Clinical microbiology reviews, 17(3), 495-508.