Antioxidant Potential of Calophyllum gracilentum: A Study on Total Phenolic Content, Total Flavonoid Content, and Free Radical Scavenging Activities

Nurr Maria Ulfa Seruji¹, Vivien Jong Yi Mian^1*, Nor Hisam Zamakshari², Thiruventhan Karunakaran³, Lee Feng Koo⁴, Wong Tze Jin⁴, Chan Thye Huat⁵

Study of potential Therapeutic activity of Calophyllum herb.

Abstract

The most prevalent phytoconstituents of medicinal and aromatic plants are phenols and flavonoids, which are responsible for antioxidant activity. This study aims to determine the total phenolic content (TPC), total flavonoid content (TFC), and free radical scavenging activity of Calophyllum gracilentum, an understudied Calophyllum species with very limited information. The stem bark extracts were prepared and examined for TPC assay by Folin–Ciocalteu method, while the TFC of the extracts was determined using aluminium chloride colorimetric method and the antioxidant activity was evaluated by free radical scavenging activity using 1,2-diphenyl-2-pricrylhydrazyl (DPPH). The extracts showed methanolic extracts exhibited the highest TPC and TFC with the value of 1542.40 ± 0.0246 mg GAE/g extract and 451.70 ± 0.0003 mg quercetin equivalents (QE)/g extract, respectively. Meanwhile, DPPH scavenging activity data revealed that all extracts had IC50 values ranging from 44.17 1.26 g/mL to 162.61 2.24 g/mL, with the methanolic extract for C. gracilentum having the highest antioxidant activity with IC50 values of 44.17 1.26 g/mL. The TPC and   TFC   assays   showed   that  C. gracilentum extracts possess a substantial to moderate phenolic and flavonoid content, while the DPPH free radical scavenging assay revealed that the extracts have strong antioxidant activity. The results demonstrate that C. gracilentum contains a high concentration of phenolic and flavonoid chemicals. These discoveries could have significant implications for the prospective use of these plants in traditional medicine and as natural antioxidant sources.

Keywords: Calophyllum; DPPH; extracts; total flavonoid content; total phenolic content.

References

Amarowicz, R., & Pegg, R. B. (2019). Chapter One - Natural antioxidants of plant origin. In I. C. F. R. Ferreira & L. Barros (Eds.), Advances in Food and Nutrition Research (Vol. 90, pp. 1-81). Academic Press. https://doi.org/https://doi.org/10.1016/bs.afnr.2019.02.011

Aminudin, N. I., Ahmad, F., & Taher, M. (2019). Antibacterial and antioxidant activities of extracts from Calophyllum ferrugineum and Calophyllum incrassatum. Malaysian Journal of Analytical Sciences, 23(4), 637-647.

Aminudin, N. I., Norazhar, N. A., & Ahmad, F. (2021). Development of a HPLC Method for Quantification of Amentoflavone in Leaf Extracts of Three Calophyllum Species.Malaysian Journal of Chemistry, 23(2), 33-39.

Blois, M. S. (1958). Antioxidant determinations by the use of a stable free radical. Nature, 181(4617), 1199-1200.

Chukwumah, Y. C., Walker, L. T., Verghese, M., & Ogutu, S. (2009). Effect of frequency and duration of ultrasonication on the extraction efficiency of selected isoflavones and trans-resveratrol from peanuts (Arachis hypogaea). Ultrasonics sonochemistry, 16(2), 293-299.

Dragusha, S., Qazimi, B., & Ejupi, V. (2023). Assessment of Total Phenolic and Flavonoid Content of Medicinal Plant Extracts from Kosovo. Journal of Medicinal plants and By-product, 12(2), 175-180. https://doi.org/10.22034/jmpb.2023.361637.1538

Dweck, A., & Meadows, T. (2002). Tamanu (Calophyllum inophyllum)-the African, Asian, polynesian and pacific panacea. International Journal of Cosmetic Science, 24(6), 341-348.

El-Lateef, H. M. A., El-Dabea, T., Khalaf, M. M., & Abu-Dief, A. M. (2023). Recent Overview of Potent Antioxidant Activity of Coordination Compounds. Antioxidants, 12(2), 213.

Geetha, G., Raju, K. N. G., Kumar, B. V., & Raja, M. G. (2012). Analytical method validation: an updated. Review. Int. J. Pharm. Biol. Sci, 1, 64-71.

 Gupta, S., & Gupta, P. (2020). The Genus Calophyllum: Review of Ethnomedicinal Uses, Phytochemistry and Pharmacology. In (pp. 215-242). Springer Singapore. https://doi.org/10.1007/978-981-15-1394-7_5

Hapsari, S., Yohed, I., Kristianita, R. A., Jadid, N., Aparamarta, H. W., & Gunawan, S. (2022). Phenolic and flavonoid compounds extraction from Calophyllum inophyllum leaves. Arabian Journal of Chemistry, 15(3), 103666. https://doi.org/https://doi.org/10.1016/j.arabjc.2021.103666

Hien, H. M., Heng, P. W., Van Thi, N., Cu, N. K. Q., & Hue, V. T. B. (2011). Development and validation of a GC-MS method for rapid determination of calophyllolide in Calophyllum inophyllum L.: A quality control approach. Chinese Journal of Natural Medicines, 9(6), 429-434.

Jadid, N., Hidayati, D., Hartanti, S. R., Arraniry, B. A., Rachman, R. Y., & Wikanta, W. (2017). Antioxidant activities of different solvent extracts of Piper retrofractum Vahl. using DPPH assay. AIP conference proceedings,

Lizazman, M. A., Karunakaran, T., & Jong, V. Y. M. (2022). Trapezifolixanthone as a common constituent in the genus Calophyllum: An insight Review. Biocatalysis and Agricultural Biotechnology, 44, 102471. https://doi.org/https://doi.org/10.1016/j.bcab.2022.102471

Ozkan, S. A. (2018). Analytical method validation: the importance for pharmaceutical analysis. Pharm Sci, 24(1), 1-2.

Phongpaichit, S., Nikom, J., Rungjindamai, N., Sakayaroj, J., Hutadilok-Towatana, N., Rukachaisirikul, V., & Kirtikara, K. (2007). Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunology & Medical Microbiology, 51(3), 517-525. https://doi.org/10.1111/j.1574-695X.2007.00331.x

Rabeta, M., & Faraniza, R. N. (2013). Total phenolic content and ferric reducing antioxidant power of the leaves and fruits of Garcinia atrovirdis and Cynometra cauliflora.International Food Research Journal, 20(4), 1691.

Salleh, W. M. N. H. W., Ahmad, F., Yen, K. H., & Sirat, H. M. (2011). Chemical compositions, antioxidant and antimicrobial activities of essential oils of Piper caninum Blume. International Journal of Molecular Sciences, 12(11), 7720-7731.

Salman, Z., Yu-Qing, J., Bin, L., Cai-Yun, P., Iqbal, C. M., Atta-ur, R., & Wei, W. (2019).

Antioxidant nature adds further therapeutic value: an updated review on natural xanthones and their glycosides. Digital Chinese Medicine, 2(3), 166-192.

Shanmugapriya, n., Chen, Y., Kanwar, J. R., & Sasidharan, S. (2017). Anticancer Activity and Molecular Mechanism of Polyphenol Rich Calophyllum inophyllum Fruit Extract in MCF-7 Breast Cancer Cells. Nutrition and Cancer, 69(8), 1308-1324. https://doi.org/10.1080/01635581.2017.1367944

Tung, Y.-T., Wu, J.-H., Kuo, Y.-H., & Chang, S.-T. (2007). Antioxidant activities of natural phenolic compounds from Acacia confusa bark. Bioresource technology, 98(5), 1120- 1123.

Wani, M. A., Prasad, S., & Prakash, O. (2019). Qualitative phytochemical analysis of various parts of bamboo (Bambusa balcooa) for possible therapeutic usages in bovine reproductive disorders. Journal of Pharmacognosy and Phytochemistry, 8(1), 217- 221.

Zailan, A. A. D., Karunakaran, T., Abu Bakar, M. H., & Mian, V. J. Y. (2022). The Malaysian genus Calophyllum (Calophyllaceae): a review on its phytochemistry and pharmacological activities. Natural Product Research, 36(17), 4569-4579.

Zamakshshari, N. H., Ee, G. C. L., Ismail, I. S., Ibrahim, Z., & Mah, S. H. (2019). Cytotoxic xanthones isolated from Calophyllum depressinervosum and Calophyllum buxifolium with antioxidant and cytotoxic activities. Food and Chemical Toxicology, 133, 110800. https://doi.org/10.1016/j.fct.2019.110800