Biological Effectiveness of Seed Extracts of Some Species From Brassicaceae Family Against Two Types of Pathogenic Bacteria
Afnan Esam Adnan 1*, Wisam Malik Dawood 1*
Journal of Angiotherapy 8(4) 1-8 https://doi.org/10.25163/angiotherapy.849515
Submitted: 29 February 2024 Revised: 02 April 2024 Published: 04 April 2024
Cruciferous Plant-derived compounds showed diverse medicinal properties, combating antibiotic resistance, providing therapeutic alternatives, and inhibiting biofilm formation in pathogenic bacteria.
Abstract
Background: Cruciferous plants are particularly rich in bioactive compounds with potential therapeutic applications. The study aimed to assess their efficacy against Staphylococcus aureus and Escherichia coli. Methods: Extraction and antimicrobial testing occurred at the University of Diyala's College of Agriculture labs from March to September 2023. Two extraction methods and varying concentrations (100%, 150%, 200%) were used. Inhibition zones were measured via agar well diffusion, and biofilm formation was evaluated using microtiter plate assays. Results: Cress seed extract showed the highest S. aureus inhibition (21.99 mm), while Radish had the lowest (5.77 mm). Chloroform extraction yielded the highest inhibition (17.00 mm), with 200% concentration being most effective (19.77 mm). For E. coli, Cress extract had the highest inhibition (15.22 mm), and chloroform extraction at 200% concentration was most effective (13.11 mm). Cress seed extract inhibited biofilm formation, especially with chloroform, while Radish and Watercress showed moderate to strong inhibition. Conclusion: Cress seed extract, particularly with chloroform extraction at higher concentrations, exhibited potent antimicrobial activity against S. aureus and E. coli, inhibiting biofilm formation. Radish and Watercress extracts also showed moderate antimicrobial effects. These findings highlight the potential of Cruciferous seed extracts as natural antimicrobial agents, warranting further research for pharmaceutical and agricultural applications.
Keywords: Medicinal plants, Antimicrobial activity, Antibiotic resistance. Cruciferous
References
Abreu, A.C., McBain, A.J., and Simoes, M. (2012). Plants as sources of new antimicrobials and resistance-modifying agents. Natural product reports, 29(9), 1007-1021.
Adikala, G. Raj ; Jayaraman, M. ; Krishnamoorthy , S. ; Chandrasekaran, M. and Venkatesalu, V.(2017). Screening of Different Extracts of Marine Macro Green Algae for Larvicidal Activity against Dengue Fever Mosquito, Aedes aegypti (Diptera: Culiadae). International Letters of Natural Sciences, Vol. 62, 44-51.
Ahmed, Ali Fadel, (2016). A molecular study of a number of Escherichia coli bacterial adhesion factors isolated from urine samples from children under five years old. Master's thesis, Ibn al-Haytham College of Education for Pure Sciences, University of Baghdad.
Al-Awadi, Salwa Saber. (1993). Study of antibacterial activity. Master's thesis/College of Veterinary Medicine - University of Baghdad.
Al-Jumaili, Hanan Thamer Najm Abdul Ali Al-Jumaili (2017) A comparative study of the virulence factors of bacteria Isolated from urinary tract infections of cardiac intensive care patients. Master's thesis, college Education for pure sciences, University of Diyala.
Allen, W. J.; Phan, G. and Waksman, G. (2012). Pilus biogenesis at the outer membrane of Gram-negative bacterial pathogens. Current opinion in structural Biolog., 22:500-506.
Almeida, C., Azevedo, N. F., Santos, S., Keevil, C. and Vieira, M. (2013). Correction: Discriminating Multi-Species Populations in Biofilms with Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA FISH) . PLoS ONE, 8(6): 101-371.
Alo, M.; Eze, U. G. and Anyim, C. (2012). In vitro Antimicrobial activities of Extracts of Magnifera indica, Caricapapaya and Psidium guajava Leaves on Samonella typhi isolates. World J. of pub.Heal.Scie.,1:1-6.
Al-Otbi, Duaa Adnan Kazem (2013). A bacteriological study of some species of Enterobacteriaceae isolated from the halls of the maternity hospital in the city of Baqubah. Master's thesis, University of Diyala, College of Education.
Al-Qaisi, Hassan (2004), Dictionary of Medicinal Herbs and Plants, Dar Al-Kutub Al-Ilmiyyah, Beirut, 6th edition.
Al-Sura Mary, Lara Mahmoud Shafiq (2014). Study of serotypes and virulence factors of bacteria Escherichia coli isolated from human urinary tract infections in Diyala Governorate Education for pure sciences.
Al-Taweel, Rana Saleh. (2016). Drug sensitivity to Staphylococcus aureus isolated from women of reproductive age. Al-Qadisiyah Journal of Pure Sciences 1(21), 50-57.
Amissah, N. A., van Dam, L., Ablordey, A., Ampomah, O. W., Prah, I., Tetteh, C. S., ... and Stienstra, Y. (2017). Epidemiology of Staphylococcus aureus in a burn unit of a tertiary care center in Ghana. PloS one, 12(7), e0181072.
Anibijuwon, I.I, and Udeze, OA. (2009), Antimicrobial Activity of Carica papaya ( pawpaw Leaf ) on Some Pathogenic Organisms of Clinical Origin from south –Western Nigeria. Ethnobotanical Leaflets 13,850 – 64.
Atlas, R. M., Parks, L. C. and Brown, A. E. (1995). Laboratory Manual of Experimental Microbiology. 1st ed. Mosby, Inc. Missouri.
Chen, Q., Xie, S., Lou, X., Cheng, S., Liu, X., Zheng, W., ... and Wang, H. (2020). Biofilm formation and prevalence of adhesion genes among Staphylococcus aureus isolates from different food sources. Microbiologyopen, 9(1), e00946.
Dari, W. A. (2019). Incidence and Antibiotics Susceptibility Pattern of Coagulase Positive and negative Staphylococci amongst (UTI) children in Children Welfare Hospital/Baghdad/IRAQ. Indian Journal of Public Health Research & Development, 10(1), 1067-1072.
Dielubauza, E. J. (2011). Urinary tract infection in women .Med. Clin. Of North America, 95(1):27-41.
Forbes, B. A., Sahm, D. F., and Weissfeld, A. S. (2007). Diagnostic microbiology. St Louis: Mosby.
Gillet, Y., Issartel, B., Vanhems, P., Fournet, J. C., Lina, G., Bes, M., ... and Etienne, J. (2002). Association between Staphylococcus aureus strains carrying gene for Panton-Valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. The Lancet, 359(9308), 753-759.
Iqbal, M. N., and Ashraf, A. (2019). Withania somnifera: Can it be a Therapeutic Alternative for Microbial Diseases in an Era of Progressive Antibiotic Resistance?. International Journal of Nanotechnology and Allied Sciences, 3(1), 16-18.
Jawetz , M and Adelberg's E.A. (2016). Midical Microbiology .27th ed ., The McGraw-Hill Companies ,Inc.New Yourk, USA.
Jiyad, Asmaa Ahmed (2016) The mixed effect of antibiotics on Staphylococcus SPP bacteria isolated from different clinical infections. Master's thesis, College of Education for Pure Sciences, University of Diyala.
Jouda, M. M. (2013). The antibacterial effect of some medicinal plant extracts and their synergistic effect with antibiotic and non-antibiotic drugs. Faculty of Science- Islamic University-Gaza.
Karpagam, P., and Manonmani, G. (2019). Antagonistic Property of Selected Herbal Combination for Substitute of Synthetic Antimicrobial Agents. Journal of Drug Delivery and herapeutics, 9(4-A), 53-56.
Kassie , F; Pool- Zobel, B; Parzefall , W. and Knasmuller, S. 1999. Genotoxic effects of benzyl isothiocyanates, a natural chemopreventive agent. Mutagenesis 14:595-604 .
Khalifa, Hassan (2009), The Book of Herbal Paradise, first edition, Dar Al-Israa for Publishing and Distribution - Amman.
Khan, F.; Shukla, I.; Rizvi, M.; Mansoor, T. and Sharma, S. C. (2017). Detection of biofilm formation in Staphylococcus aureus .Does it have a role in treatment of MRSA Infection. Trends in Med. Res. Academic J., 6(2): 116-123.
Mazokopokis EE,papadakis J. and Apapadomanoloki MG, (2007), Effects of12 months treatment with L-seleno methionine on seruman anti Tpo levels in patients with it ashimotos thyroiditis.Thyroid17:609-612.
Moteeb, Sh. H.(2018). Quantitative and Qualitative Assayes OF Bacterial Biofilm Produced By Pseudomonas aeruginosa AND Klebsiella spp. J. of al-anbar university for pure science : Vol.2: No.3.
Namasivayam, S. K. R., Christo, B. B., Arasu, S. K., Kumar, K. A. M., and Deepak, K. (2013). Anti-biofilm effect of biogenic silver nanoparticles coated medical devices against biofilm of clinical isolate of Staphylococcus aureus. Global Journal of Medical Research, 13(3), 1-7.
Obeidat, M., Shatnawi, M., Al-Alawi, M., Al-Zubi, E., Al-Dmoor, H., Al-Qudah, M., El-Qudah, J. and Qtri, I. (2012). Antimicrobial Activity of Crude Extracts of Same Plant Leaves. Res. J. of Microbiology ., 7:59-67.
Pincus, D. H. (2006). Microbial identification using the bioMérieux Vitek® 2 system. Encyclopedia of rapid microbiological methods, 1, 1-32.
Pulipatis,S ., Babu , P.S.,Narasu, M.L.,and Anush , N. (2017) An over view on urinary traet infection J. Med. Plant, 5 , 50-56 .
Rashmi, D.; Pathak, V. and Kumar, R. (2019). Effect of Ricinus communis L. on microorganisms: Advantages and dis advantages Int. J. Curr. Microbiol. App. Sci., 8(4): 878-884.
Sabah, Zaman Atiya Sabah. (2018). Bacteriological and molecular study of Escherichia coli bacteria Isolated from urinary tract infections in displaced persons camps and investigating their bacteriophages. Master's thesis, College of Science, University of Diyala.
Sofowora, A.; Ogunbodede, E. and Onayade, A. (2013). The role and place of medicinal plants in the strategies for disease prevention. Afr. J. Tradit. Complement. Altern. Med., 10(5) :210-229.
Stahil, R. (1969). Thin layer chromatoghraphy: A laboratory hand book. 2nd edition. Traslated by ashworth, M. R. Springer. Berlin, Heidelbery . N. Y.
Tajbakhsh, E.; Ahmadi, P. Abedpour-Dehkordi, E.; Arbab-Soleimani, N. and Khamesipour, F. (2016). Biofilm Formation, Antimicrobial Susceptibility, Serogroups and Virulence Genes of Uropathogenic E. coli Isolated from Clinical Samples in Iran. Antimicrob Resist Infect Control. 5(11): 1-8.
Tepe , B. ; Donmez , E. ; Unlu , M. ; Candan , F. ; Daferera , D. ; Vardar – Unlu , G. ; Polissiou , M. and Sokmen , A. (2004), Antimicrobial and antioxidative activities of the essential oils and methanol extracts of the Salvia cryptantha (Monthbret et Aucher ex Benth.) and Salvia multicaulis ( Vol1), Food Chemistry. 84(4) : 519 – 525.
Tiwari, N., López-Redondo, M., Miguel-Romero, L., Kulhankova, K., Cahill, M. P., Tran, P. M., ... and Tuffs, S. W. (2020). The SrrAB two-component system regulates Staphylococcus aureus pathogenicity through redox sensitive cysteines. Proceedings of the National Academy of Sciences, 117(20), 10989-10999.
Valle Jr, D. L., Andrade, J. I., Puzon, J. J. M., Cabrera, E. C., and Rivera, W. L. (2015). Antibacterial activities of ethanol extracts of Philippine medicinal plants against multidrug-resistant bacteria. Asian pacific journal of tropical biomedicine, 5(7), 532-540.
World Health Organization (WHO). (2020). World health statistics 2020: monitoring health for the SDGs, sustainable development goals ISBN 978-92-4- 000510-5 (electronic version).
Zidane, Israa Ali Al-Qazzaz, Abdul Karim Abdul Razzaq, and Muhammad Ali Sadiq. (2014). The effect of the combination Antibiotics on clinically isolated Staphylococcus aureus bacteria. University Journal Baghdad Science, Department of Biotechnology, College of Science, University of Baghdad, 6 (4).
Al-Khalidi, Zuwaida Kazem Khudair. (2016). Technological study of some Gram-negative Bacterial species isolated from vaginal infections. Master's thesis, College of Education for Pure Sciences, University of Diyala.
View Dimensions
View Altmetric
Save
Citation
View
Share