In vitro Antibacterial Activity of Seribu kuman Leaf (Rhinacanthus nasutus (l.) Kurz) Extracts Against Staphylococcus aureus and Pseudomonas aeruginosa
Betty Fitriyasti 1*, Siska Ferilda 2, Widia Sari 3, Muhammad Rizki Saputra 4, Heng Yen Khong 5*
Journal of Angiotherapy 8(1) 1-7 https://doi.org/10.25163/angiotherapy.819433
Submitted: 20 November 2023 Revised: 20 January 2024 Published: 23 January 2024
Rhinacanthus nasutus extract inhibits the growth of Staphylococcus aureus and could serve as an alternative in treating infections caused by S. aureus.
Abstract
Seribu Kuman leaves (Rhinacanthus nasutus (L.) Kurz) are the herb most commonly used in traditional medicine to treat various diseases. The presence of secondary metabolites, including flavonoids, alkaloids, and phenolics, has led to its known antibacterial activity. This study aims to verify the antibacterial activity of Seribu Kuman leaf extract against Staphylococcus aureus and Pseudomonas aeruginosa. This study involved experimental design using the Kirby-Bauer method, inoculating Mueller-Hilton Agar (MHA) test media containing S. aureus and P. aeroginosa bacteria with Seribu Kuman leaves ethanol extracts at concentrations of 15%, 30%, and 60%. Penicillin and ciprofloxacin were used as positive controls, whereas sterile aquabidest was used as a negative control for the study. The findings of this study showed that Seribu Kuman (R. nasutus) ethanol extract, at a concentration of 60%, presented the most significant inhibition zone against S. aureus, measuring 13.6 mm. In contrast, no inhibition zone was observed for P. aeroginosa. It can be concluded that Seribu Kuman (R. nasutus) ethanol extract can inhibit the growth of S. aureus but not P. aeruginosa. So, Seribu Kuman leaves (R. nasutus) ethanol extract could be an alternative for treating infections caused by S. aureus.
Keywords: Antibacterial, Seribu Kuman leaves, Rhinacanthus nasutus (L.) Kurz, Staphylococcus aureus, Pseudomonas aeruginosa.
References
Abdulsalami, MS., Aina, VO., Ibrahim, MB., Adejo, GO., Audu, G. (2016). Comparative antibacterial study of aqueous and ethanolic leaf extracts of Annona muricata. Journal of Natural Sciences Research,6(3), 141-6 https://api.semanticscholar.org/CorpusID:54617059.
Andry, M., Khairani, TN., Tarigan, RE., Nursafwati.., Nasution, MA., Tambunan, IK, et al. (2023). Antibacterial activity test of sweet corn (Zea mays L.) ethanol extract on Escherichia coli and Staphylococcus epidermidis bacteria. Manganite Journal of Chemistry and Education,2(1), 15-23. https://doi.org/10.58330/manganite.v2i1.301.
Anggita, A., Fakhrurrazi, F., Harris, A. (2018). Uji aktivitas antibakteri ekstrak etanol daun putri malu (Mimosa pudica) terhadap bakteri Pseudomonas aeruginosa [Test of the antibacterial activity of ethanol extract of putrimalu (Mimosa pudica) leaves against Pseudomonas aeruginosa bacteria]. Jurnal Ilmiah Mahasiswa Veteriner,2(3), 411-88. https://doi.org/10.21157/jim%20vet..v2i3.8572.
Breijyeh, Z., Jubeh, B., Karaman, R. (2020). Resistance of Gram-negative bacteria to current antibacterial agents and approaches to resolve it. Molecules,25(6), 1340. http://dx.doi.org/10.3390/molecules25061340.
Brimson, JM., Prasanth, MI., Malar, DS., Brimson, S., Tencomnao, T. (2020). Rhinacanthus nasutus "tea" infusions and the medicinal benefits of the constituent phytochemicals. Nutrients,12(12), 3776-94. https://doi.org/10.3390/nu12123776.
Chairunnisa, S., Wartini, NM., Suhendra, L. (2019). Pengaruh suhu dan waktu maserasi terhadap karakteristik ekstrak daun bidara (Ziziphus mauritiana L.) sebagai sumber saponin [The effect of temperature and maceration time on the characteristics of bidara leaf extract (Ziziphus mauritiana L.) as a source of saponin]. Jurnal Rekayasa Dan Manajemen Agroindustri ISSN,2503, 488x. https://doi.org/10.24843/JRMA.2019.v07.i04.p07.
Deni, J., Pangalila, FJ. (2019). Hubungan keberhasilan terapi pneumonia nosokomial resisten Pseudomonas aeruginosa dan Acinetobacter baumannii dengan dosis Karbapenem di ICU RS Royal Taruma periode 2012-2017 [Correlation of the success of therapy for resistant nosocomial pneumonia Pseudomonas aeruginosa and Acinetobacter baumannii with Carbapenem doses in the ICU at Royal Taruma Hospital for the period 2012-2017]. Tarumanagara Medical Journal,1(3), 532-43. https://doi.org/10.24912/tmj.v2i1.5865.
Febria, FA., Rahmadeni, Y., Bachtiar, A. (2021). Antibacterial potential ethanol extract of kayu racun leaf (Rhinacanthus nasutus) against Staphylococcus aureus and Methicillin Resistant Staphylococcus aureus. Jurnal Pendidikan Matematika dan IPA,12(2), 180-8. http://dx.doi.org/10.26418/jpmipa.v12i2.46801.
https://doi.org/10.1155/2019/7428593
https://doi.org/10.14203/beritabiologi.v17i2.3086
https://doi.org/10.14710/ijfst.13.1.1-6
https://doi.org/10.15294/lifesci.v10i2.54441
https://doi.org/10.24843/JRMA.2019.v07.i04.p07
https://doi.org/10.26418/jpmipa.v12i2.46801
https://doi.org/10.31001/biomedika.v12i1.462
https://doi.org/10.31289/biolink.v6i2.2731
https://doi.org/10.33541/jpvol6Iss2pp102
https://doi.org/10.3390/molecules25061340
https://doi.org/10.3390/nu12123776
https://doi.org/10.35799/jbl.10.1.2020.27978
https://doi.org/10.36733/medicamento.v7i2.2177
https://doi.org/10.37089/jofar.vi0.102
https://doi.org/10.37311/jsscr.v2i1.2665
https://doi.org/10.5530/pj.2017.2.35
https://doi.org/10.5530/pj.2021.13.164
https://doi.org/10.7860/JCDR/2017/30085.10347
Irawan, C., Elya, B., Hanafi, M., Saputri, FC. (2021). Application of ultrasound-assisted extraction on the stem bark of Rhinachantus nasutus (L.) Kurz, total phenolic, and its potential as antioxidant and inhibitor of alpha-glucosidase enzyme activity. Pharmacognosy Journal,13(5), 1297-303. https://doi.org/10.5530/pj.2021.13.164.
Jamilatun, M. (2019). Uji resistensi antibiotik Staphylococcus aureus isolat kolam renang [Antibiotic resistance test of Staphylococcus aureus swimming pool isolates]. Biomedika,12(1), 1-8. https://doi.org/10.31001/biomedika.v12i1.462.
Johari, MA., Khong, HY. (2019). Total phenolic content and antioxidant and antibacterial activities of Pereskia bleo. Advances in Pharmacological Sciences,2019, 7428593. doi: https://doi.org/10.1155/2019/7428593.
Magani, AK., Tallei, TE., Kolondam, BJ. (2020). Uji antibakteri nanopartikel kitosan terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli [Antibacterial test of chitosan nanoparticles against the growth of Staphylococcus aureus and Escherichia coli bacteria]. Jurnal Bios Logos,10(1), 7-12. https://doi.org/10.35799/jbl.10.1.2020.27978.
Nanthakumar, R., Udhayasankar, M., Ashadevi, V., Arumugasamy, K., Shalimol, A. (2014). In vitro antimicrobial activity of aqueous and ethanol extracts of Rhinacanthus nasutus - A medicinal plant International Journal of Pharmaceutical, Chemical & Biological Sciences,4(1). 10.7860/JCDR/2022/56581.16713.
Noviyanto, F., Hodijah, S., Yusransyah, Y. (2020). Aktivitas ekstrak daun Bangle (Zingiber purpureum roxb.) terhadap pertumbuhan bakteri Pseudomonas aeruginosa [Activity of Bangle leaf extract (Zingiber purpureum roxb.) on the growth of Pseudomonas aeruginosa bacteria]. Journal Syifa Sciences and Clinical Research,2(1), 31-8. https://doi.org/10.37311/jsscr.v2i1.2665.
Pratiwi, R. (2017). Mekanisme pertahanan bakteri patogen terhadap antibiotik [Defense mechanisms of pathogenic bacteria against antibiotics]. Jurnal Pro-Life,4(3), 418-29. https://doi.org/10.33541/jpvol6Iss2pp102.
Purnomo, HY., Azzahra, F. (2021). Uji aktivitas antibakteri ekstrak etanol daun alpukat (Persea americana Mill.) terhadap bakteri Pseudomonas aeruginosa [Test of the antibacterial activity of ethanol extract of avocado leaves (Persea americana Mill.) against Pseudomonas aeruginosa bacteria]. Jurnal Kefarmasian Akfarindo, 7-14. https://doi.org/10.37089/jofar.vi0.102.
Rani, GN., Budumuru, R., Bandaru, NR. (2017). Antimicrobial activity of honey with special reference to Methicillin Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA). Journal of Clinical and Diagnostic Research,11(8), DC05-DC8. https://doi.org/10.7860/JCDR/2017/30085.10347.
Rarassari, MA., Maftuch, HN. (2016). Phytochemicals and antibacterial activities of soursop leaf (Annona muricata) against Edwardsiella tarda (in vitro). Journal of Life Science and Biomedicine,6(1), 06-9. https://www.jlsb.science-line.com/vol-6-no-1-jan-2016.html
Sadikin, N., Bintari, S., Widiatningrum, T., Dewi, P. (2021). Isolasi, karakteristik, dan uji aktivitas antibakteri dari bakteri endofit Daun Kelor (Moringa oleifera) [Isolation, characteristics, and antibacterial activity test of the endophytic bacteria of Moringa Leaves (Moringa oleifera)]. Life Science,10(2), 109-19. https://doi.org/10.15294/lifesci.v10i2.54441.
Septiani., Dewi, NH., Wijayanti, I. (2017). Aktivitas antibakteri ekstrak lamun (Cymodocea rotundata) terhadap Bakteri Staphylococcus aureus dan Eschericia coli [Antibacterial activity of seagrass extract (Cymodocea rotundata) against Staphylococcus aureus and Eschericia coli bacteria]. Saintek Perikanan,13(1), 1-6. https://doi.org/10.14710/ijfst.13.1.1-6.
Singh, N., Gupta, S., Rathore, V. (2017). Comparative antimicrobial study of ethanolic extract of leaf and rhizome of Curcuma longa Linn. Pharmacognosy Journal,9(2), 208-12. https://doi.org/10.5530/pj.2017.2.35.
Sukertiasih, NK., Megawati, F., Meriyani, H., Sanjaya, DA. (2021). Studi retrospektif gambaran resistensi bakteri terhadap antibiotik [Retrospective study of bacterial resistance to antibiotics]. Jurnal Ilmiah Medicamento,7(2), 108-11. https://doi.org/10.36733/medicamento.v7i2.2177.
Sunarti, S., Paninsari, D. (2020). Daya hambat daun Manukan (Rhinacanthus nasutus (L) Kurz) terhadap bakteri Staphylococcus aureus secara in vitro [Inhibitory power of Manukan (Rhinacanthus nasutus (L) Kurz) leaves against Staphylococcus aureus bacteria in vitro]. BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan),6(2), 106-13. https://doi.org/10.31289/biolink.v6i2.2731.
Trimanto, T., Dwiyanti, D., Indriyani, S. (2018). Morfologi, anatomi dan uji histokimia rimpang Curcuma aeruginosa Roxb; Curcuma longa L. dan Curcuma heyneana Valeton dan Zijp. Berita Biologi,17(2), 123-33. https://doi.org/10.14203/beritabiologi.v17i2.3086.
Widyananda, GAD., Mahendra, AN., Jawi, IM. (2021). Efek antibakteri ekstrak etanol daun sirsak (Annona muricata L.) muda dan tua terhadap Pseudomonas aeruginosa ATCC 9027 [Antibacterial effect of ethanol extract of young and old soursop leaves (Annona muricata L.) against Pseudomonas aeruginosa ATCC 9027]. Intisari Sains Medis,12(1), 212-8. https://doi.org/10.15562/ism.v12i1.915
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