Advancing Personalized Cancer Care: Integrating CRISPR/Cas9 with Next-Generation Sequencing Technologies

Md Abdur Rahman; Md Abu Bakar; Md Mahedi Hasan; Syeda Anjuman Ara; Md Moshiur; Debashis Chandra; Tufael6*

doi:10.25163/biosciences.6110004

Biopharmaceuticals and medical sciences | Online ISSN 3064-9226

REVIEWS (Open Access)

Advancing Personalized Cancer Care: Integrating CRISPR/Cas9 with Next-Generation Sequencing Technologies

Md Abdur Rahman Biswash¹, Md Abu Bakar Siddique², Md Mahedi Hasan Shabuj¹, Syeda Anjuman Ara Aunni³, Md Moshiur Rahman⁴, Debashis Chandra Das⁵, Tufael^6*

+ Author Affiliations

Journal of Precision Biosciences 6 (1) 1-14 https://doi.org/10.25163/biosciences.6110004

Submitted: 22 April 2024 Revised: 11 June 2024 Published: 13 June 2024

Abstract

Background: Cancer arises from a complex interplay of genetic and epigenetic abnormalities, presenting significant challenges for conventional therapies. This complexity underscores the urgent need for innovative therapeutic approaches. The integration of the CRISPR/Cas9 system with next-generation sequencing (NGS) presents a promising avenue for rapid identification, validation, and targeting of critical therapeutic targets. Methods: Personalized medicine leverages genetic, phenotypic, and environmental data to tailor healthcare solutions, moving beyond the limitations of standardized treatments. Advances in cancer genome sequencing have facilitated this shift, with NGS offering advantages such as minimal sample requirements and the ability to identify novel biomarkers. Tumor profiling, along with cell-free DNA analysis, proteomics, and RNA studies, enhances our understanding of immunological responses and informs treatment strategies. Results: The CRISPR/Cas9 system enables precise targeting of genetic alterations in tumor cells, providing a mechanism to disrupt genetic pathways responsible for tumorigenesis and metastasis. This targeted approach enhances the potential for more effective and personalized therapies. Combining NGS and CRISPR/Cas9 aims to match treatments to specific tumor profiles and develop bespoke therapeutic strategies tailored to individual tumors. This review highlights the transformative potential of CRISPR/Cas9 and NGS in advancing personalized cancer treatment.

Keywords: CRISPR/Cas9, Next Generation Sequencing (NGS), Personalized Medicine, Cancer Treatment, Liquid Biopsy

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