Evaluation of Chemicals Fungicides And Botanicals Extract On The Suppression Of Sclerotium Rolfsii Causes Wilt Of Sunflower (Helianthus annuus L.).
Tanni Das 1†, Palash Maitra1†, Pulak Maitra 2*, Dipa mandal 2, Md. Shahidul Islam 1
†Equal contributors
Energy Environment & Economy 2(1) 1-8 https://doi.org/10.25163/energy.2110155
Submitted: 22 October 2024 Revised: 11 December 2024 Published: 14 December 2024
Abstract
The present investigation was conducted to evaluate the efficacy of chemicals and botanicals agent on the suppression of Sclerotium rolfsii causes wilt of sunflower. The fungal pathogen was identified based on both cultural and molecular characteristics. The rDNA sequence also revealed the 99%-100% similarity with the reference sequences confirming fungal pathogen identity. The antifungal activity of four commercially available fungicides viz., Copper oxychloride, Carbendazim, 20% Azoxistrobin + 12.5% Difenokonazol and 3% Hexakonazol + 22%Trisiclazol and five plant extracts viz., Neem, Garlic, Zinger, Biskathali and Datura were evaluated against Sclerotium rolfsii isolated from infected sunflower plants. The results revealed that all the chemicals and plant extracts significantly inhibited the mycelial growth of Sclerotium rolfsii with varying degree in comparison with control. Among the fungicides, 20% Azoxistrobin + 12.5% Difenokonazol (100%) and 3% Hexakonazol + 22%Trisiclazol (100%) showed the best performance in controlling the mycelial growth of Sclerotium rolfsii. Among the plant extracts, both Garlic (52.96%) and Zinger (52.96%) significantly inhibited the mycelial growth of Sclerotium rolfsii.
Keywords: Sclerotium rolfsii; Wilt Disease; Pathogenicity; Fungicides; Botanical extract; ITS sequencing.
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