Angiogenesis, Inflammation & Therapeutics | Online ISSN  2207-872X
RESEARCH ARTICLE   (Open Access)

Cannabis Sativa: A Remedy for Convulsion by Inhibition of GABAA Receptor and Significantly Delayed Onset of Seizure Latency and Death - An Experimental Validation and Molecular Docking

Ben Enoluomen Ehigiator1, SamuelKelechi Mobisson2, Iheanyichukwu Wopara3*, Chinonye Cynthia Chibuife1, Uwaezuoke Chukwuekele Awarajih3, Harrison Ogheneochuko Eruotor3

+ Author Affiliations

Journal of Angiotherapy 5(1) 207-217 https://doi.org/10.25163/angiotherapy.51212710730301021

Submitted: 07 September 2021  Revised: 27 October 2021  Published: 30 October 2021 

Abstract

This study aimed to investigate the anticonvulsant potentials of Cannabis sativa and its possible mechanism using the in vivo and in-silico models. A total of 20 male albino mice was used for this study and were divided randomly into five groups. Group 1 as negative control receiving 0.2ml 0f 10% ethanol only, and groups 2 and 3, received 200mg/kg/ body weight low dose of C. Sativa extract and 400mg/kg bw low dose of C. sativa extract, while groups 4 received 50 mg/kg body weight of phenobarbitone and group 5 received 50 mg/kg body weight of phenobarbitone+ 200 mg/kg body weight of the extract. Administration of C. Sativa extract was done via orogastric feeding; GABA-ergicseizure, was induced by administration of 80 mg/kg/bodyweight of Pentylenetetrazole (PTZ) via the intra-peritoneal route to all the groups. This was done about thirty minutes after the administration of treatment agents.  Each animal was observed individually for one hour to determine the onset of muscular jerks and tonic hind limb extensions and death.  Animal study results revealed that C. sativa may likely possess’ anticonvulsant potentials as It is significantly seizure latency and death in PTZ treated mice. For the in silico studies, about 49 compounds of C. sativa were obtained from the PubChem library and were docked on various targets site of proteins; Glutaminase, 4-Aminobtyrate Aminotransferase, Perioxisome proliferator Activated receptor (PPAR), Succinic semialdehyde dehydrogenase, Gama amino butyric acid –A receptor (GABA-A), Histone deacetylases (HDAC), Phosphoribosyl pyrophosphate amido transferase and human GAFT-1. Furthermore, the in-silico study revealed that compounds like Cannabisin A and D showed anticonvulsant potential activation of GABA.

Key Words: Convulsions, Anticonvulsant, Cannabis Sativa, in-silico studies, Glutaminase  

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