Acute and Sub- chronic Toxicity Study of Encapsulation of Combine Plants Extract of Ficus deltoidea and Gynocthodes sublanceolata in Balb/c Mice Model
Rafida Kh. Ahmed A, M. Syarhabilahmad A, Midhat Nabil A, Fouad Saleih R. Al –Suede B,*
Journal of Angiotherapy 3(1) 147-155 https://doi.org/10.25163/angiotherapy.31208511930191219
Submitted: 19 August 2019 Revised: 25 December 2019 Published: 30 December 2019
Abstract
This study aimed to evaluate the safety of dose range of encapsulated extract with Nano hydroxyapatite from a combination of Ficus deltoidea and Gynocthodes sublanceolata leaves by inducing "acute and sub-chronic toxicity" based on an animal model. A single dose (300, 2000 and 4000 mg/kg) of encapsulated combination extracts were administrated orally in the acute toxicity trial and the toxic effects were assessed up to 72 h post-treatment. While, in the sub-chronic study, the encapsulated combination extract was given orally at doses of 600 12 and 1000 mg/kg for 28 days. Hematological and histopathological analysis of some vital organs were evaluated. Neither in the acute toxicity trial nor the sub-chronic toxicity groups, was mortality observed throughout the experimental period. Significant increasing was seen in the SGOT and SGPT level following the administration of tested encapsulated plant extract (600, 1000 mg/kg) and histological evaluation showed the normal tissue limit of liver, kidney and spleen. It was concluded that the oral treatment with encapsulated plant extract did not appear adverse side effect and it is safe to use for therapeutic purposes.
Key Words: Toxicity, Medicinal herb, Safety, Ficus deltoidea and Gynocthodes sublanceolata
References
Adeneye AA, Olagunju JA. Preliminary hypoglycemic and hypolipidemic activities of the aqueous seed extract of Carica papaya Linn in Wistar rats. Biol Med 2009; 1(1): 1-10.
Alsarhan A, Sultana N, Al-Khatib A, Abdul Kadir MR. Review on some Malaysian traditional medicinal plants with therapeutic properties. J Basic Appl Sci 2014; 10: 149-59.
Arsad SS, Mohd Esa N, Hamzah H, Othman F. Evaluation of acute, subacute and subchronic oral toxicity of Rhaphidophora 314 Muhammad Kifayatullah et al./Journal of Acute Disease 2015; 4(4): 309–315 decursiva (Roxb.) Schott extract in male Sprague Dawley rats. J Med Plant Res 2013; 7: 3030-40.
Bernard, S. A., &Olayinka, O. A. (2010). Search for a novel antioxidant, Anti-inflammatory /analgesic or anti-proliferative drug: Cucurbitacins hold the aceJ. Med. Plant. Res, 4(25), 2821-2826.
Donkor K, Okine LNK, Abotsi WKM, Woode E. Acute and subchronic toxicity studies of aqueous extract of root bark of Cassia sieberiana DC in rodents. J Appl Pharm Sci 2014; http://dx.doi.org/10.7324/JAPS.2014.40415.
Elham Farsi, Armaghan Shafaei, Sook Yee Hor, Mohamed B. Khadeer Ahamed, Mun Fei Yam, Mohd Z. Asmawi, and Zhari Ismail Genotoxicity and acute and subchronic toxicity studies of a standardized methanolic extract of Ficus deltoidea leaves Clinics (Sao Paulo). 2013 Jun; 68(6): 865–875.
Harkness SE, Wagner JE. Biologia e Clínica de Coelhos Roedores. 3rd edition. São Paulo:Livraria Roca; 1993. pp. 48–55.
Hench, L. L. (1991). Bioceramics: From concept to clinic. Journal of the American Ceramic Society, 74 (7): p.1487-1510.
Ilyanie Y, Wong TW, Choo CY. Evaluation of hypoglycemic activity and toxicity profiles of the leaves of Ficus deltoidea in rodents. J Complement Integr Med. 2011;8(1) doi: 10.2202/1553-3840.1469.
Ivone Margarida Nunes Ferreira Vieira PeresEncapsulation of Active Compounds: Particle Characterization, Loading E?ciency and StabilityDissertation presented for obtaining the degree of: Doctor of Philosophy in Chemical and Biological Engineering by University of Porto, 2011.
K.H. Suk, S.C.B. Gopinath, Drug Encapsulated Nanoparticles for Treating Targeted Cells, Curr. Med. Chem. 24 (2017) 3310–3321. doi:10.2174/0929867324666170502122444.
K.H. Suk, S.C.B. Gopinath, P. Anbu, T. Lakshmipriya, Cellulose nanoparticles encapsulated cow urine for effective inhibition of pathogens, Powder Technol. 328 (2018) 140–147. doi:10.1016/j.powtec.2018.01.010.
Kausar MW, Moeed K, Asif N, Rizwi F, Raza S. Correlation of bilirubin with liver enzymes in patients of falciparum malaria. Int J Pathol 2010; 8(2): 63-7.
Kavitha, K Sujatha and S Manoharan Development, Characterization and Antidiabetic Potentials of Nilgirianthus ciliatus Nees Derived Nanoparticles Kavitha et al., J Nanomedine Biotherapeutic 372 Discovry journal 2017, 7:2 DOI: 10.4172/2155-983X.1000152.
Kunimatsu T, Yamada T, Miyata K, Yabushita S, Seki T, Okuno Y, et al. Evaluation for reliability and feasibility of the draft protocol for the enhanced rat 28-day subacute study (OECD Guideline 407) using androgen antagonist flutamide. Toxicology 2004; 200(1): 77-89.
M.E. Foo, P. Anbu, S.C.B. Gopinath, T. Lakshmipriya, C.G. Lee, H.S. Yun, M.N.A. Uda, A.R.W. Yaakub, Antimicrobial activity of functionalized single walled carbon nanotube with herbal extract of Hempedu bumi, Surf. Interface Anal. 50 (2018) 354–361. doi:10.1002/sia.6375.
M.R. Mucalo, D.L. Foster, A Method for Avoiding the Xanthoproteicassociated Discolouration in Reprecipitated ( Nitric-acid-digested ) Hydroxyapatite Prepared from Mammalian Bone Tissue, 77 (2004) 509–
Muhammad Kifayatullah , Mohd. Shahimi Mustafa, Pinaki Sengupta, Md. Moklesur Rahman Sarker, Arindam Das, Sreemoy Kanti Das Evaluation of the acute and sub-acute toxicity of the ethanolic extract of Pericampylus glaucus (Lam.) Merr. in BALB/c mice Journal of Acute Disease journal homepage: www.jadweb.org Journal of Acute Disease 2015; 4(4): 309–315.
Mythilypriya R, Shanthi P, Sachdanandam P. Oral acute and subacute toxicity studies with Kalpaamruthaa, a modified indigenous preparation, on rats. J Health Sci 2007; 53(4): 351-8.
N.S. Ismail, S.C.B. Gopinath, Enhanced antibacterial effect by antibiotic loaded starch nanoparticle, J. Assoc. Arab Univ. Basic Appl. Sci. 24 (2017) 136–140. doi:10.1016/j.jaubas.2016.10.005.
P. Matouskova, I. Marova, J. Bokrova, P. Benesova, Effect of encapsulation on antimicrobial activity of herbal extracts with lysozyme, Food Technol. Biotechnol. 54 (2016) 304–316. doi:10.17113/ftb.54.03.16.4413.
R. Arunachalam, V. Rajeev, V. Vedam, S. Ganapathy, J. Dhanavel, Effect of Using the Combination of Tween 80 and Ethanol on the Forming and Physical Stability of Microemulsion of Eucalyptus Oil as Antibacterial., J. Young Pharm. 9 (2017) S1–S4. doi:10.5530/jyp.2017.
R. Renuka, S. Rajasekaran, G. Radhakrishnan, Electrochemically synthesized polymer of the plant substance embelin (2,5-dihydroxy-3undecyl-1,4-benzoquinone), Appl. Biochem. Biotechnol. - Part A Enzym. Eng. Biotechnol. 96 (2001) 83–92. doi:10.1385/ABAB:96:1-3:083.
Rhiouani H, El-Hilaly J, Israili ZH, Lyoussi B. Acute and subchronic toxicity of an aqueous extract of the leaves of Herniaria glabeous extract of the leaves of Herniaria glabra in rodents. J Ethnopharmacol 2008; 118(3): 378-86.
S. Anniebell, S.C.B. Gopinath, Polymer Conjugated Gold Nanoparticles in Biomedical Applications, (2018) 1–13. doi:10.2174/0929867324666170116123633.
S.C.B. Gopinath, C.S. Goh, M. Citartan, T. Lakshmipriya, M.K. Arshad, M.F. Fatin, A.R. Ruslinda, U. Hashim, S. V Chinni, Micro-Encapsulation of Antibiotic in Cellulose Nanoparticle Inhibits Bac- teria, Micro Nanosyst. (2016) 1–6. doi:10.2174/18764029086661607201040.
T. Cao, W. Tang, J. Zhao, L. Qin, C. Lan, A novel drug delivery carrier based on α-eleostearic acid grafted hydroxyapatite composite, J. Bionic Eng. 11 (2014) 125–133. doi:10.1016/S1672-6529(14)60027-5.
T. Theivasanthi, F.L. Anne Christma, A.J. Toyin, S.C.B. Gopinath, R. Ravichandran, Synthesis and characterization of cotton fiber-based nanocellulose, Int. J. Biol. Macromol. 109 (2018) 832–836. doi:10.1016/j.ijbiomac.2017.11.054.
Vaghasiya YK, Shukla VJ, Chanda S. Acute oral toxicity study of Pluchea arguta Boiss extract in mice. J Pharmacol Toxicol 2011; 6(2): 113-23.
Walum E. Acute oral toxicity. Environ Health Perspect 1998; 106(Suppl 2): 497-503.
Yehya, A. H., Asif, M., Kaur, G., Hassan, L. E., Al-Suede, F. S., Majid, A. M. A., & Oon, C. E. (2019). Toxicological studies of Orthosiphon stamineus (Misai Kucing) standardized ethanol extract in com7bination with gemcitabine in athymic nude mice model. Journal of advanced research, 15, 59-68.
Z.N. Al-Sokanee, A.A.H. Toabi, M.J. Al-assadi, E.A.S. Alassadi, The Drug Release Study of Ceftriaxone from Porous Hydroxyapatite Scaffolds, AAPS PharmSciTech. 10 (2009) 772–779. doi:10.1208/s12249-009-9265-7.
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