EMAN RESEARCH PUBLISHING | Journal | <p>Physico-chemical Characterization of Indigenous <em>Streptomyces</em> and Influence of pH on Antimicrobial Activity</p>
Microbial and anti-microbial compound biology
RESEARCH ARTICLE   (Open Access)

Physico-chemical Characterization of Indigenous Streptomyces and Influence of pH on Antimicrobial Activity

Maruf Abonya, Avijit Banika, Md. Asaduzzaman Shishirb, Noor Jahan Akterc, Muhammad Ekhlas Uddina,Suvamoy Dattaa*

+ Author Affiliations

Microbial Bioactives 1(2) 059-067 https://doi.org/10.25163/microbbioacts.12009A3010021118

Submitted: 30 May 2018  Revised: 03 October 2018  Published: 02 November 2018 

The ability of the Streptomyces isolates in tolerating wide range of growth conditions would be of special advantage and the influence of pH in pathogen specific antimicrobial production could enhance the chances of obtaining diverse antimicrobials.

Abstract


Background: Emergence of multi-drug resistant pathogens has afflicted the population of developing countries like Bangladesh in recent years for which a sustainable holistic combating approach is required. Since Streptomyces is a source of numerous bioactive molecules, the study was aimed at physico-chemical characterization of 8 indigenousStreptomyces isolates of Bangladesh. Methods: Tolerance of Streptomyces isolates to different growth conditions was assessed at temperature range 4 °C to 60 °C, pH range 3 to 11 and salinity up to 15% of NaCl concentration. Ability of isolates in utilizing different carbohydrates was checked through media of single sugar as sole carbon and energy source. The antimicrobial activity of the isolates against four pathogens was assayed with culture supernatant obtained from 5 different pH levels. The data was analyzed statistically by software R version 3.4.1. Results: All the isolates grew optimally in the temperature range of 20- 40 °C, pH range of 5- 9 and salinity of 1% NaCl concentration although certain isolates tolerated up to 60 °C and 10% of salinity. Based on the sugar profiles, the isolates were allocated into different biotypes and their relatedness was found with S. mutabilis (Ca), S. subrutilis(Cb1) and S. mirabilis (Cb2) as positioned at same clusters in the dendrogram. The antimicrobial molecules produced by the Streptomyces isolates were not heat stable and denatured by ethanol, hence presumed as protein. The maximum antagonism was recorded against E. coli by isolate B-5 at pH 6 (18 mm), against S. typhimurium by A- 9 at pH 9 (20 mm), against S. aureus by B-7 at pH 5 (18 mm) and against B. cereus by B- 7 at pH 8 and 9 (18 mm) as well as by D-5 at pH 7 (18 mm). It was also deduced that the pH as a growth condition significantly influenced the production of pathogen specific antimicrobial compounds by the Streptomyces isolates. Conclusion: The ability of the Streptomyces isolates in tolerating wide range of growth conditions would be of special advantage and the influence of pH in pathogen specific antimicrobial production could enhance the chances of obtaining diverse antimicrobials.

Keywords: Streptomyces, Multi-drug resistance, Antimicrobial activityGrowth parameters, Biotyping, pH.

Significance: Developing potential antimicrobials from indigenous Streptomyces.

Abbreviations: ISP- International Streptomyces Project, ATCC- American Type Culture Collection, spp- species, SCDA- Soybean Casein Digest agar, ZOI- Zone of Inhibition, MDR-Multi drug resistant.

References


A, E. M., & M, M. M. M. (2012). Antibacterial and insecticidal activity of actinomycetes isolated from sandy soil of (Cairo-Egypt). Egyptian Academic Journal of Biological Sciences, 4(1), 53–67. 
https://doi.org/10.21608/eajbsg.2012.16661

Abony, M., Alam, Kh. M., Banik, A., Jannat, B., & Datta, S. (2017). Isolation and Identification of Antibiotic Producing Microorganisms of Actinobacteriaceae Family from Soil Samples of Dhaka and Comilla. Journal of Primeasia University, 1(2), 13-21.

Ahmad, M. S., El-Gendy, A. O., Ahmed, R. R., Hassan, H. M., El-Kabbany, H. M., & Merdash, A. G. (2017). Exploring the Antimicrobial and Antitumor Potentials of Streptomyces sp. AGM12-1 Isolated from Egyptian Soil. Frontiers in Microbiology, 8, 438. 
https://doi.org/10.3389/fmicb.2017.00438

Al-Bari, M. A. A., Bhuiyan, M. S. A., Flores, M. E., Petrosyan, P., García-Varela, M., & Ul Islam, M. A. (2005). Streptomyces bangladeshensis sp. nov., isolated from soil, which produces bis-(2-ethylhexyl)phthalate. International Journal of Systematic and Evolutionary Microbiology, 55(5), 1973–1977. 
https://doi.org/10.1099/ijs.0.63516-0

Anderson, A. S., & Wellington, E. (2001). The taxonomy of Streptomyces and related genera. International Journal of Systematic and Evolutionary Microbiology, 51(3), 797–814. 
https://doi.org/10.1099/00207713-51-3-797

Barai, L., Saha, M. R., Rahman, T., Khandaker, T., Dutta, S., Hasan, R., & Haq, J. A. (2017). Antibiotic resistance?: Situation analysis in a tertiary care hospital of Bangladesh Original Article Antibiotic Resistance?: Situation Analysis In a Tertiary Care Hospital of Bangladesh, 34(December), 15–19.

Bérdy, J. (2005). Bioactive Microbial Metabolites. The Journal of Antibiotics, 58(1), 1–26. 
https://doi.org/10.1038/ja.2005.1

Bizuye, A., Moges, F., & Andualem, B. (2013). Isolation and screening of antibiotic producing actinomycetes from soils in Gondar town, North West Ethiopia. Asian Pacific Journal of Tropical Disease, 3(5), 375–381. 
https://doi.org/10.1016/S2222-1808(13)60087-0

Crits-Christoph, A., Diamond, S., Butterfield, C. N., Thomas, B. C., & Banfield, J. F. (2018). Novel soil bacteria possess diverse genes for secondary metabolite biosynthesis. Nature, 558(7710), 440–444. 
https://doi.org/10.1038/s41586-018-0207-y

Faiz, M. A., & Ariful, B. (2011). Antimicrobial resistance: Bangladesh experience. Regional Health Forum, 15(1), 1–8.

Ferdous, U. T., Shishir, M. A., Khan, S. N., & Hoq, M. M. (2018). Bacillus spp.: Attractive Sources of Anti-cancer and Anti-proliferative Biomolecules. Microbial Bioactives, 1(1), E033–E045. 
https://doi.org/10.25163/microbbioacts.11005B0408130818

Hoque, M. M., Rattila, S., Shishir, M. A., Bari, M. L., Inatsu, Y., & Kawamoto, S. (2011). Antibacterial Activity of Ethanol Extract of Betel Leaf (Piper betle L.) Against Some Food Borne Pathogens. Bangladesh Journal of Microbiology, 28(2), 58–63. 
https://doi.org/10.3329/bjm.v28i2.11817

Jiang, G., Zhang, Y. Y., Powell, M. M., Zhang, P., Zuo, R., Zhang, Y. Y., … Ding, Y. (2018). High-Yield Production of Herbicidal Thaxtomins and Thaxtomin Analogs in a Nonpathogenic Streptomyces Strain. Applied and Environmental Microbiology, 84(11). 
https://doi.org/10.1128/AEM.00164-18

Khatun, M. F., Haque, M. U., & Islam, M. A. U. (2018). Antibacterial and Cytotoxic Activities of Crude Ethyl Acetate Extract of Streptomyces sp. FEAI-1 Isolated From Soil Samples of Rajshahi, Bangladesh. Bangladesh Pharmaceutical Journal, 20(2), 188. 
https://doi.org/10.3329/bpj.v20i2.37884

Lee, L.-H., Chan, K.-G., Stach, J., Wellington, E. M. H., & Goh, B.-H. (2018). Editorial: The Search for Biological Active Agent(s) From Actinobacteria. Frontiers in Microbiology, 9, 824. 
https://doi.org/10.3389/fmicb.2018.00824

MacGowan, A., & Macnaughton, E. (2017). Antibiotic resistance. Medicine, 45(10), 622–628. 
https://doi.org/10.1016/j.mpmed.2017.07.006

Martinko, J. M., & Madigan, M. M. (2005). Brock biology of microorganisms (11th ed.). London: Pearson Prentice Hall, c2006.

Montgomery, D. C. (2006). Design and Analysis of Experiments. Technometrics, 48(1), 158–158. 
https://doi.org/10.1198/tech.2006.s372

Mustafa, O., A., U. T., & Cem, A. (2004). Antibacterial activity of some actinomycetes isolated from farming soils of Turkey. African Journal of Biotechnology, 3(9), 441–446. 
https://doi.org/10.5897/AJB2004.000-2087

Nishat, S. A., & Alam, M. M. (2017). Screening of antibiotic-producing Streptomyces from marine sediments of Bangladesh. Bangabandhu Sheikh Mujib Medical University Journal, 10(4), 255. 
https://doi.org/10.3329/bsmmuj.v10i4.34403

Pandey, A., Shukla, A., & Majumdar, S. K. (2005). Utilization of carbon and nitrogen sources by Streptomyceskanamyceticus M 27 for the production of an Anti bacterial antibiotic. African Journal of Biotechnology, 4(9).

Priyanka, K. (2011). Isolation, characterization and identification of Actinobacteria of Mangrove ecosystem, Bhitarkanika, Odisha. National Institute Of Technology. Retrieved from http://ethesis.nitrkl.ac.in/2337/1/thesis(priyanka).pdf

Progress on antibiotic resistance. (2018). Nature, 562(7727), 307–307. 
https://doi.org/10.1038/d41586-018-07031-7

Rahman, M. S., & Huda, S. (2014). Antimicrobial resistance and related issues: An overview of Bangladesh situation. Bangladesh Journal of Pharmacology, 9(2), 218–224. 
https://doi.org/10.3329/bjp.v9i2.18831

S. Ningthoujam, D., & Sanasam, S. (2011). Studies on Bioactive Actinomycetes in a Niche Biotope, Nambul River in Manipur, India. Journal of Microbial & Biochemical Technology, 01(S6). 
https://doi.org/10.4172/1948-5948.S6-001

Ser, H.-L., Tan, L. T.-H., Law, J. W.-F., Chan, K.-G., Duangjai, A., Saokaew, S., … Lee, L.-H. (2017). Focused Review: Cytotoxic and Antioxidant Potentials of Mangrove-Derived Streptomyces. Frontiers in Microbiology, 8: 2065. 
https://doi.org/10.3389/fmicb.2017.02065

Sharmin, T., Rahman, M. A., Sayeed, A., Anisuzzaman, M., & Islam, M. A. U. (2017). Streptomyces BanglaensisSp. Nov., Isolated from Soils of Rajshahi, Bangladesh Producing Actinomycin D. Specialty Journal of Biological Sciences, 3(2), 32–39.

Shishir, M. A., Mamun, M. Al, Mian, M. M., Ferdous, U. T., Akter, N. J., Suravi, R. S., … Kabir, M. E. (2018). Prevalence of Vibrio cholerae in Coastal Alternative Supplies of Drinking Water and Association with Bacillus-Like Spore Formers. Frontiers in Public Health, 6(50), 1-11. 
https://doi.org/10.3389/fpubh.2018.00050

Shishir, M. A., Pervin, S., Sultana, M., Khan, S. N., & Hoq, M. M. (2015). Genetic Diversity of Indigenous Bacillus thuringiensis Strains by RAPD-PCR to Combat Pest Resistance. Bt Research, 6(8), 1–16. 
https://doi.org/10.5376/bt.2015.06.0008

Singh, L. S., Mazumder, S., & Bora, T. C. (2009). Optimisation of process parameters for growth and bioactive metabolite produced by a salt-tolerant and alkaliphilic actinomycete, Streptomyces tanashiensis strain A2D. Journal de Mycologie Medicale. 19(4), 225-233 
https://doi.org/10.1016/j.mycmed.2009.07.006

V, V., & S, J. (2018). Bioactive potential from Marine sponge Callyspongia diffusa associated Pseudomonusfluorescens BCPBMS-1 and Penicillum citrinum. Microbial Bioactives, 1(1), 8–13. 
https://doi.org/10.25163/microbbioacts.11002A2221300318

van Belkum, A., Bachmann, T. T., Lüdke, G., Lisby, J. G., Kahlmeter, G., Mohess, A., … Dunne, W. M. (2018). Developmental roadmap for antimicrobial susceptibility testing systems. Nature Reviews Microbiology. 
https://doi.org/10.1038/s41579-018-0098-9

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