Real-Time PCR Diagnosis of COVID-19 in Al Anbar Province, Iraq
Mohammed A. Hamad 1*, Firas R. Jameel 1, Noor Najm Abdullah 1, Muhaidi MJ 2
Journal of Angiotherapy 8(4) 1-6 https://doi.org/10.25163/angiotherapy.849545
Submitted: 31 January 2024 Revised: 03 April 2024 Published: 07 April 2024
Accurate diagnosis with real-time PCR is essential for pandemic control. Understanding transmission dynamics aids in formulating effective public health measures.
Abstract
Background: Genomic sequencing has advanced COVID-19 understanding, facilitating therapeutic assessment and guiding treatment strategies. Precise diagnosis is vital for grasping transmission dynamics and detecting novel variants. The study aimed to investigate the molecular attributes, mutations, and diagnostic methodologies of the coronavirus (SARS-CoV-2) pandemic. Methods: Real-time PCR was utilized for COVID-19 diagnosis in Al Anbar province. Samples underwent RNA extraction and RT-PCR amplification. Between February and April 2020, 245 samples were collected from Anbar province, initially yielding negative results via rtPCR. Data on infection rates, gender disparities, and clinical outcomes were analyzed. Results: In 2020, the infection rate was 14%, reducing to 11% in 2021. Men exhibited higher infection rates (70%) compared to women (30%). Clinical data indicated varying severity and fatality rates between sexes. Conclusion: While RT-PCR remains widely accepted, alternative diagnostic methods show promise. Further research is essential for comprehensive COVID-19 understanding and improving diagnostic accuracy. The study underscores ongoing research's significance in combating viral epidemics, emphasizing the need for comprehensive understanding and adaptive strategies to mitigate mortality risks.
Keywords: COVID-19, Real-time PCR, diagnosis, Transmission dynamics, Viral variants
References
Chan, J. F. W., Chan, K. H., Choi, G. K. Y., To, K. K. W., Tse, H., Cai, J. P., et al. (2013). Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: Implications for disease pathogenesis and clinical manifestation. Journal of Infectious Diseases, 207(11), 1743–1752.
Chan, J. F. W., Choi, G. K. Y., Tsang, A. K. L., Tee, K. M., Lam, H. Y., Yip, C. C. Y., et al. (2015). Development and evaluation of novel real-time reverse transcription-PCR assays with locked nucleic acid probes targeting leader sequences of human-pathogenic coronaviruses. Journal of Clinical Microbiology, 53(8), 2722–2726.
Dong, X., Liu, L., Tu, Y., Zhang, J., Miao, G., Zhang, L., et al. (Year). Rapid PCR powered by microfluidics: A quick review under the background of COVID-19 pandemic. Trends in Analytical Chemistry, 143.
Friedrich, R., Rappold, E., Bogdan, C., & Held, J. (2018). Comparative analysis of the Wako β-glucan test and the Fungitell assay for diagnosis of candidemia and Pneumocystis jirovecii pneumonia. Journal of Clinical Microbiology, 56(9).
Hag-Ali, M., AlShamsi, A. S., Boeijen, L., Mahmmod, Y., Manzoor, R., Rutten, H., et al. (2021). The detection dogs test is more sensitive than real-time PCR in screening for SARS-CoV-2. Communications Biology, 4(1).
Hamad, M. A., Jameel, F. R., Fouad Ali, L., & Fouad Ali, M. (2021). Molecular and epidemiological study on COVID-19 in Iraq. Biochemical and Cellular Archives, 2(2), 4085–4092. Available from: https://orcid.org/0000-0002-2375-8872
Mukherjee, S., & Pahan, K. (Year). Is COVID-19 Gender-sensitive? [Link to the source]
Najaflou, N., Monajjemi, M., Attarikhasraghi, N., Kandemirli, F., & Mollaamin, F. (2021). The specification of observed COVID-19 in England: A review of auto-mutation. Biointerface Research in Applied Chemistry, 11(6), 14794–14808.
Singh, J. (2020). COVID-19 and Its Impact on Society. Electronic Research Journal of Social Sciences and Humanities, 2(1), 168–172.
To, K. W., Tsang, T. Y., Yip, C. Y., Chan, K. H., Wu, T. C., Chan, J. M., et al. (Year). Consistent detection of 2019 novel coronavirus in saliva. [Link to the source]
Yousif, A. A., Suliman, N., Ali, A. M., Abuelhassan, A. O., Fawzi, E. M., Elbasheer, S. A., et al. (2020). Is a Real Time-Polymerase Chain Reaction a Reliable Confirmatory Test for COVID-19? Journal of Blood Disorders & Transfusion, 11(6), 442. Available from: https://www.researchgate.net/publication/346309011
Zhou, J., Chu, H., Li, C., Wong, B. H. Y., Cheng, Z. S., Poon, V. K. M., et al. (2014). Active replication of Middle East respiratory syndrome coronavirus and aberrant induction of inflammatory cytokines and chemokines in human macrophages: Implications for pathogenesis. Journal of Infectious Diseases, 209(9), 1331–1342.
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