Phytochemical Composition, Antioxidant, and Anti-Inflammatory Activities of Laurus nobilis Leaf Extracts Using Solvent Fractionation

Naima Layachi; 2*; Amel Berrezeg; 4

doi:10.25163/angiotherapy.9110175

Integrative Biomedical Research | Online ISSN 2207-872X

RESEARCH ARTICLE (Open Access)

Previous Next Contents Vol 9 (1)

Phytochemical Composition, Antioxidant, and Anti-Inflammatory Activities of Laurus nobilis Leaf Extracts Using Solvent Fractionation

Naima Layachi ^{1, 2}*, Amel Berrezeg ^{3, 4}

+ Author Affiliations

Journal of Angiotherapy 9 (1) 1-6 https://doi.org/10.25163/angiotherapy.9110175

Submitted: 23 September 2024 Revised: 30 December 2024 Published: 02 January 2025

Abstract

Background: Laurus nobilis L. (Lauraceae), widely known for its culinary and medicinal uses, contains a variety of bioactive compounds. Recent interest has focused on its potential as a natural source of antioxidant and anti-inflammatory agents. This study aimed to evaluate the phytochemical composition and investigate the antioxidant and anti-inflammatory potentials of various solvent extracts of L. nobilis leaves collected from Merahna, Souk-Ahras, Algeria. Methods: Leaves of L. nobilis were collected, identified, and dried before extraction using methanol-water (85:15 v/v). Subsequent fractionation was carried out using solvents of increasing polarity: chloroform, diethyl ether, n-butanol, and ethyl acetate. Qualitative phytochemical screening was performed using established protocols, and total phenolic content (TPC) was quantified via the Folin–Ciocalteu method. Antioxidant activity was assessed using the DPPH radical scavenging assay, while anti-inflammatory potential was evaluated by the inhibition of albumin denaturation. Results: Phytochemical screening revealed the presence of flavonoids, tannins, alkaloids, anthraquinones, and steroids. Among all fractions, the ethyl acetate (AcEt) extract exhibited the highest TPC (214.04 ± 0.02 mg GAE/g dry matter), followed by the butanol and methanol extracts. The AcEt extract also showed significant DPPH radical scavenging activity (IC50 = 39.24 ± 0.01 μg/mL), while the aqueous extract was less effective (IC50 = 315.80 ± 0.01 μg/mL). In the anti-inflammatory assay, AcEt extract demonstrated the highest inhibition of protein denaturation (98.28%) with an IC50 value of 65.68 ± 0.42 μg/mL, closely comparable to diclofenac (62.57 ± 0.22 μg/mL). Conclusion: The ethyl acetate extract of Laurus nobilis leaves possesses remarkable antioxidant and anti-inflammatory activities, attributable to its rich phenolic content. These findings support the traditional use of L. nobilis and highlight its potential application in the development of natural therapeutic agents.

Keywords: Laurus nobilis, phytochemicals, antioxidant activity, anti-inflammatory, ethyl acetate extract

References

Cho, M., Lee, H.S., Kang, I.J., Won, M.H., & You, S. (2011). Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed. Food Chemistry, 127(3), 999-1006.

Dobroslavic, E.; Repajic, M.; Dragovic-Uzelac, V.; Elez Garofulic, I.(2022). Isolation of Laurus nobilis Leaf Polyphenols: A Review on Current Techniques and Future Perspectives. Foods , 11, 235.

Elshafi e, H.S. and Camele, I. 2017. An overview of the biological eff ects of some mediterranean essential oils on human health. BioMed Research International, 2017:9268468.

Hafizoglu, H.; Reunanen, M. Studies on the Components of Laurus nobilis from Turkey with Special Reference to Laurel Berry Eur. J. Lipid Sci.Technol. 1993, 95, 304–308.

Kancherla, N.; Dhakshinamoothi, A.; Chitra, K.; Komaram, R.B.(2019). Preliminary Analysis of Phytoconstituents and Evaluation of Anthelminthic Property of Cayratia auriculata (In Vitro). Mædica .14, 350–356.

Kaurinovic B, Popovic M, Vlaisavljevic S. (2019). In vitro and in vivo effects of Laurus nobilis L. leaf extracts. Molecules, 15(5), 3378-3390.

Kilic, A.; Hafizoglu, H.; Kollmannsberger, H.; Nitz, S. (2004).Volatile constituents and key odorants in leaves, buds, flowers, and fruits of Laurus nobilis. L. J. Agric. Food Chem., 52, 1601–1606.

López, A., Rico, M., Rivero, A., & Suárez de Tangil, M. (2011). The effects of solvents on the phenolic contents and antioxidant activity of Stypocaulon scoparium algae extracts. Food Chemistry, 125(3), 1104-1109.

Maajida, A.; Geetha, R.: Lakshmi, T. (2019). Evaluation of anti-inflammatory action of Laurus nobilis-an in vitro study. GeethaRVetal.,Int.J.Res.Pharm.Sci., 10, (2), 1209-1213.

Macharia, J.M.; Mwangi, R.W.; Rozmann, N.; Zsolt, K.; Varjas, T., Uchechukwu, P.O., Wagara, I.N., & Raposa, B.L.(2022). Medicinal plants with anti-colorectal cancer bioactive compounds:Potential game-changers in colorectal cancer management. Biomedicine&Pharmacotherapy,153,113383.

Mansour, O.; Darwish, M.; Ismail, G.; Douba, Z.(2018) Review Study on the Physiological Properties and Chemical Composition of the Laurus nobilis. Pharma. Chem. J. , 5, 225–231.

Markham, K.R. (1982). Technics of flavonoids identification. Academic Press.1982

Meriga, B.; Mopuri, R.; MuraliKrishma, T. (2012): Insecticidal antimicrobial and antioxidant activities of bulb extracts of Allium sativum. Asian Pacific Journal of Tropical Medicine .5: 391-395.

Milner, J.A. A historical perspective on garlic and cancer. J. Nutr. (2001), 131, 1027S–1031S.

Morita, T.; Jinno, K.; Kawagishi, H.; Arimoto, Y.; Suganuma, H.; Inakuma, T.; Sugiyama, K.(2003). Hepatoprotective effect of myristicin from nutmeg (Myristica fragrans) on lipopolysaccharide/d-galactosamine-induced liver injury. J. Agric. Food Chem., 51, 1560–1565.

Mythili, K.; Reddy, C.U.; Chamundeeswari, D.; Manna, P.K.(2014) Determination of Total Phenol, Alkaloid, Flavonoid and Tannin in Different Extracts of Calanthe Triplicata. Res. Rev. J. Pharmacogn. Phytochem., 2, 40–44.

Nava, D.E. ; Savaris, M. ; Silveira Neto, S. ; & Zucchi, R.A. (2021). Outbreak of Lauritrioza alacris (Flor, 1861)(Hemiptera, Triozidae) in a commercial plantation of bay laurel (Laurus nobilis L., Lauraceae) in Brazil. Entomological Communications, 3, 2675-1305.

Okmu, D. E. (2005). Phytochemicals, vitamins and mineral contents of two Nigerian medicinal plants. – International Journal of Molecular Sciences 14: 375-381. ISSN: 1994-4624.

Pavela, R.(2016) History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects—A Review. Plant Prot. Sci. 4, 229–241.

Sakat, S.;Juvekar A.R.;Gambhire MN.(2010).In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. International Journal of Pharma and Pharmacological Sciences; 2(1):146-155.2010

Singleton, V.L.; Rossi, J.A. (1965).Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Jour-nal of Enology and Viticulture 16(3):144-158.1965

Škerget, M.; Kotnik, P.; Hadolin, M.; Hraš, A. R., Simonic; M., ; Knez, Ž. (2005). Phenols, proanthocyanidins, flavones and flavonols in some plant materials and their antioxidant activities. Food chemistry, 89(2), 191-198. 2005

Soussi, M.; Fadil, M.; Yaagoubi, W.A.; Benjelloun, M.; El Ghadraoui, L. (2022). Simultaneous Optimization of Phenolic Compounds and Antioxidant Abilities of Moroccan Pimpinella anisum Extracts Using Mixture Design Methodology. Processes, 10(12), Article 12.

Unuofin, J.O. Lebelo, S.L. (2020). Antioxidant Effects and Mechanisms of Medicinal Plants and Their Bioactive Compounds for the Prevention and Treatment of Type 2 Diabetes : An Updated Review. Oxidative Medicine and Cellular Longevity,e1356893.

Velavan, S.(2015). Phytochemical techniques-a review. World Journal of Science and Research, 1(2), 80-91.

Export Citation

View Dimensions

View Plumx

View Altmetric

0
Save

0
Citation

83
View

0
Share