Journal of Angiotherapy
Angiogenesis, Inflammation & Therapeutics | Online ISSN  2207-872X
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RESEARCH ARTICLE   (Open Access)
Contents Vol 8 (9)

Milk-Based Ointment Accelerates Wound Healing: A Comparative Study in Rabbits

Chiranjeev Singh 1*, Kunal Chandrakar 1

+ Author Affiliations

Journal of Angiotherapy 8 (9) 1-7 https://doi.org/10.25163/angiotherapy.899883

Submitted: 29 June 2024 Revised: 03 September 2024  Published: 10 September 2024 

Abstract

Background: Wound healing is a multi-stage process that restores tissue integrity following injury. While chemical treatments like phenytoin aid in healing, traditional remedies, such as milk-based applications, remain underexplored. Milk, particularly its proteins, is known for promoting tissue repair and collagen synthesis. This study evaluated the wound-healing efficacy of a milk-based ointment (MO) on open skin wounds in rabbits. Methods: Male New Zealand white rabbits were randomly assigned to six groups, including negative controls, placebo (eucerin), and 1% phenytoin, alongside three MO groups (2%, 5%, 10% concentrations). The treatments were applied twice daily for 14 days. Wound healing was assessed through macroscopic analysis, tensile strength measurement, hydroxyproline levels, and histological examination. Results: The 5% MO-treated group exhibited the fastest wound healing, achieving full closure in 15 days, outperforming both controls and the phenytoin-treated group (17 days). Hydroxyproline levels and tensile strength were significantly higher in the 5% MO group, indicating enhanced collagen production and stronger tissue regeneration. Histological analysis further confirmed accelerated epithelialization and granulation in MO-treated groups. Conclusion: The study demonstrated that milk-based ointments, particularly at a 5% concentration, significantly enhance wound healing, suggesting their potential as cost-effective, biocompatible alternatives to conventional chemical treatments like phenytoin. Further research is needed to explore clinical applications in humans.

Keywords: Milk-based ointment, Wound healing, Tissue regeneration, Collagen production, Hydroxyproline

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