Assessing Listeria Monocytogenes Survival and Growth in Skim, Partial Skim, and Full-Fat Milk under Refrigeration

Marwa Mahmood; Fouad K. Alsammarraie

doi:10.25163/angiotherapy.849640

Angiogenesis, Inflammation & Therapeutics | Online ISSN 2207-872X

RESEARCH ARTICLE (Open Access)

Previous Next Contents Vol 8 (4)

Assessing Listeria Monocytogenes Survival and Growth in Skim, Partial Skim, and Full-Fat Milk under Refrigeration

Marwa Mahmood ^1*, Fouad K. Alsammarraie ¹

+ Author Affiliations

Journal of Angiotherapy 8 (4) 1-8 https://doi.org/10.25163/angiotherapy.849640

Submitted: 28 January 2024 Revised: 08 April 2024 Published: 12 April 2024

Abstract

Background: Milk is a global fundamental dietary staple cherished for its nutritional richness and versatility. However, despite pasteurization efforts, its susceptibility to bacterial contamination, particularly by pathogens like Listeria monocytogenes (L. monocytogenes), poses a significant concern. This study explored how components within cow milk influence L. monocytogenes, particularly in safeguarding bacterial cells against damage or destruction during refrigeration. Method: Forty samples were employed, comprising 30 milk varieties (skim, partial skim, and full-fat) and 10 phosphate buffer solution (PBS) samples serving as controls. Each sample was inoculated with L. monocytogenes and stored in a refrigerator at 4-5°C for one week. Results: The highest survival rate on tryptose agar (TA) was observed in the full-fat milk group at 99.28% after 7 days, showing no significant difference (p ≥0.05) from baseline. Conversely, the control and skim milk groups exhibited the lowest survival rates, at 27.23% and 58.95%, respectively, after the same storage period. Furthermore, an elevated percentage of injured bacterial cells on tryptose salt agar (TSA) was found in the partial skim and skim milk groups (46.02% and 57.14%, respectively), compared to the full-fat milk group (25.85%), with significant differences (p ≤ 0.05) across all time points (0, 1, 3, 5, and 7 days). Conclusion: The study underscores L. monocytogenes' resilience and capacity to proliferate even under adverse conditions such as refrigeration. Moreover, it highlights the pathogen's ability to withstand high-salt environments, as evidenced by its performance on TSA media containing 5.5% NaCl.

Keywords: Milk, Listeria Monocytogenes, Refrigeration, Bacterial Survival, Bacterial Count.

References

Abdalla, M. S., AL-Khatib, G. M. and Alwan, M. J. (2004) ‘Effect Isolation and identification of Listeria monocytogenes from aborted women with studing of its pathogenic’, The Iraqi Journal of Veterinary Medicine, 28(1), pp. 95–108.

Al-maqasisi, L. A. and Al-Samarai, F. R. (2023) ‘Effect of the Cold Plasma and Aqueous Grape Seeds Extract on Minced Beef’, European Chemical Bulletin, 12(1), pp. 419–433.

Alsammarraie, F. K. et al. (2018) ‘Green synthesis of silver nanoparticles using turmeric extracts and investigation of their antibacterial activities’, Colloids and Surfaces B: Biointerfaces, 171, pp. 398–405.

Alsammarraie, F. K., Lin, M. and Mustapha, A. (2023) ‘Green synthesis of silver nanomaterials and evaluation of their antibacterial and antioxidant effectiveness in chicken meat’, Food Bioscience, 56, p. 103332.

AL-Shamary, A. H. A. (2009) ‘Detection of Listeria monocytogenes in

AL-Shamary, A. H. A. (2010) ‘Prevalence of Listeria in ice creams in Baghdad province’, The Iraqi Journal of Veterinary Medicine, 34(2), pp. 39–44.

Doyle, M. P. et al. (1987) ‘Survival of Listeria monocytogenes in milk during high-temperature, short-time pasteurization’, Applied and Environmental Microbiology, 53(7), pp. 1433–1438.

Duguma, B. and Janssens, G. P. J. (2015) ‘Assessment of dairy farmers’ hygienic milking practices and awareness of cattle and milk-borne zoonoses in Jimma, Ethiopia’, Assessment, 45.

El-Kest, S. E. and Marth, E. H. (1991) ‘Injury and Death of Frozen Listeria monocytogenes as Affected by Glycerol and Milk Components’, Journal of Dairy Science, 74(4), pp. 1201–1208. doi: 10.3168/jds.S0022- 0302(91)78274-X.

El-Kest, S. E. and Marth, E. H. (1992) ‘Freezing of Listeria monocytogenes and other microorganisms: a review’, Journal of food protection, 55(8), pp. 639–648.

Farber, J. M. et al. (1988) ‘Thermal resistance of Listeria monocytogenes in inoculated and naturally contaminated raw milk’, International Journal of Food Microbiology, 7(4), pp. 277–286. doi: 10.1016/0168- 1605(88)90054-2?

Fathi, I. A. (2019) ‘Surveillance for Contamination of Salad with Listeria Monocytogenes in Some of Baghdad Restaurants’, AL-Kindy College Medical Journal, 15(2), pp. 4–8.

Gany Kszaal Atshan and Al-Graibawi, M. A. (2018) ‘Molecular characterization and antimicrobial susceptibility of Listeria monocytogenes isolated from human placentas and non-human samples.’,

Hoque, Sheikh Ariful ; Sultana, Ummay Nasrin; Hossain, Tania. (2018). Pasteurization of milk through direct heating up to75°C over a kitchen stove at home. Microbial Bioactives, 1(1), pages 001–007.

Ibrahim, Z. I. (2017) ‘Study the ultrastructure changes in the liver of mice post infection with Listeria monocytogenes: Zainab I. Ibrahim1 Moutaz AW Abdul–Mounam2 and Laith AM Alsufi3’, The Iraqi Journal of Veterinary Medicine, 41(1), pp. 155–159.

Jia, Z. et al. (2021) ‘Dynamic kinetic analysis of growth of Listeria monocytogenes in pasteurized cow milk’, Journal of Dairy Science, 104(3), pp. 2654–2667.

Kamana, O. et al. (2014) ‘Microbiological quality and safety assessment of the Rwandan milk and dairy chain’, Journal of Food Protection, 77(2), pp. 299–307.

Kim, S. and Kang, D. (2015) ‘Effect of milk fat content on the performance of ohmic heating for inactivation of Escherichia coli O157: H7, Salmonella enterica Serovar Typhimurium and Listeria monocytogenes’, Journal of applied microbiology, 119(2), pp. 475–486.

Kivaria, F. M., Noordhuizen, J. and Kapaga, A. M. (2006) ‘Prospects and constraints of smallholder dairy husbandry in the Dar es Salaam region, Tanzania’, Outlook on AGRICULTURE, 35(3), pp. 209–215.

Lima, J. S. et al. (2021) ‘Growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions’, Ciência Rural, 51, p. e20200967.

Michelon, D. et al. (2016) ‘Growth potential assessment of Listeria in milk fat products by challenge testing’, Journal of Food Safety, 36(2), pp. 260– 270.

OJVR Online Journal of Veterinary Research, 22(2), pp. 104–114. Goff, H. D. et al. (2013) ‘Ice cream structure’, Ice cream, pp. 313–352. Harding, F. (1995) Milk quality. Springer.

Posada-Izquierdo, G. D. et al. (2021) ‘Modelling the effect of salt concentration on the fate of Listeria monocytogenes isolated from Costa Rican fresh cheeses’, Foods, 10(8), p. 1722.

Pricope-Ciolacu, L. et al. (2013) ‘The effect of milk components and storage conditions on the virulence of Listeria monocytogenes as determined by a Caco-2 cell assay’, International journal of food microbiology, 166(1), pp. 59–64.

Ribeiro, A. C. et al. (2023) ‘Listeria monocytogenes: An inconvenient hurdle for the dairy industry’, Dairy, 4(2), pp. 316–344.

Rosenow, E. M. and Marth, E. H. (1987) ‘Growth of Listeria monocytogenes in Skim, Whole and Chocolate Milk, and in Whipping Cream during Incubation at 4, 8, 13, 21 and 35°C’, Journal of Food Protection, 50(6), pp. 452–460. doi: 10.4315/0362-028x-50.6.452.

Ryser, E. T. (2021) ‘Listeria’, in Foodborne Infections and Intoxications. Elsevier, pp. 201–220.

Ryser, E. T. and Marth, E. H. (2007) Listeria, listeriosis, and food safety. CRC press.

Salman, A. et al. (2021) ‘Effect of Temperature at the Shelf Life of Yogurt and the Role of <i>Listeria monocytogenes</i> and <i>E. coli</i> Inoculated in Unflavoured and Flavoured Yogurts’, International Journal of Food Engineering and Technology, 5(2), p. 91. doi: 10.11648/j.ijfet.20210502.18.

Siderakou, D. et al. (2021) ‘Assessing the survival and sublethal injury kinetics of Listeria monocytogenes under different food processing-related stresses’, International Journal of Food Microbiology, 346, p. 109159.

Soft-Cheese and Sweet Yogurt Produced Locally in Baghdad: Ali HA AL- Shamary and Najim H. Najim’, The Iraqi Journal of Veterinary Medicine, 33(2), pp. 78–83.

Taylor, M. H. and Zhu, M.-J. (2021) ‘Control of Listeria monocytogenes in low-moisture foods’, Trends in Food Science & Technology, 116, pp. 802–814.

Vasavada, P. C. (1988) ‘Pathogenic bacteria in milk—a review’, Journal of dairy science, 71(10), pp. 2809–2816.

Wiktorczyk-Kapischke, N. et al. (2023) ‘Assessment of the influence of selected stress factors on the growth and survival of Listeria monocytogenes’, BMC microbiology, 23(1), p. 27.

Wilkins, P. O., Bourgeois, R. and Murray, R. G. E. (1972) ‘Psychrotrophic properties of Listeria monocytogenes’, Canadian Journal of Microbiology, 18(5), pp. 543–551.

Yang, Y. et al. (2022) ‘Survival and thermal resistance of Listeria monocytogenes in dry and hydrated nonfat dry milk and whole milk powder during extended storage’, International Dairy Journal, 129, p. 105338.

Yu, M. et al. (2023) ‘Effect of low temperature on the resistance of Listeria monocytogenes and Escherichia coli O157: H7 to acid electrolyzed water’, Food Research International, 168, p. 112776.

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