Microbial Bioactives
Microbial Bioactives | Online ISSN 2209-2161
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Volume 1 Number 1 2019
RESEARCH ARTICLE (Open Access)
Phytase Production from a Novel Klebsiella sp. on Wheat Bran for Animal Feed Digestion
Dilruba Akter a, Md. Murad Khan b, Md. Mahmuduzzaman Mian a, Shakila Nargis Khan a, Md. Mozammel Hoq a
Microbial Bioactives 1 (1) 014-021 https://doi.org/10.25163/microbbioacts.11004A0423100718
Submitted: 03 March 2018 Revised: 23 May 2018 Published: 02 April 2019
Abstract
Background: The present study was aimed to isolate phytase producing bacteria and optimize the physicochemical parameters of their phytase production. Materials and methods: Four bacterial isolates (Phs4, Phs5, Phs6, and Phs8), based on clear zone formation on phytase screening medium, were selected and tested for finding out the highest phytase producing strain. The production of phytase was then optimized and its biochemical properties were determined to judge the applicability of phytase as a digestive aid in animal feed. Results: The 4 bacterial isolates (Phs4, Phs5, Phs6 and Phs8) were identified by morphological, cultural, biochemical and molecular characterization as Burkholderia cepacia, Escherichia coli, Klebsiella pneumoniae and Klebsiella sp. respectively. Of these isolates, Phs8 (Klebsiella sp.) was found to produce maximum phytase in shake culture in a basal medium containing Na-phytate at 37oC and pH 5.5 after 72 hours of incubation. The omission of Na-phytate from the medium almost completely abolished the phytase production capacity of the isolate and thus signified its important role as an inducer. Among the different complex carbon sources, viz., glucose, wheat bran, rice bran and chickpea, maximum phytase production (94 unit/ ml) was obtained with wheat bran under comparable cultivation conditions. The phytase works best at a temperature of 37oC and pH of 4.0 with a wide temperature stability (more than 80% activity up to 80oC) and wide pH stability (more than 80% activity within a range of 3-8). Although Zn2+, Co2+, and Fe2+ slightly increased the phytase activity Cu2+ and Mg2+ strongly inhibited the enzyme. Conclusion: The present findings will be very useful for the development of a bioprocess of the enzyme for its large-scale production at the pilot and finally at the commercial level.
Keywords: Phytase; animal feed; Klebsiella sp.; enzyme activity.
Abbreviations: PSM, phytase screening medium; PPM, phytase production medium; LB, Luria-Bertani; RPM, rotation per minute; PCR, polymerase chain reaction; dNTPs, deoxyribonucleotides; EDTA, ethylenediaminetetraacetic acid; EtBr, ethidium bromide; KIA, Kligler iron agar; MIU, motility indole urease test; VP, Voges–Proskauer test.
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