Efficacy of Egyptian Parasitic Nematodes, Heterorhabditis bacteriophora (BA1) and Steinernema carpocapsae (BA2) in Bio-control of Economically Important Pests
Mona A. Hussein 1*
Microbial Bioactives 4(1) 150-155 https://doi.org/10.25163/microbbioacts.412140A0608151221
Submitted: 06 November 2021 Revised: 10 December 2021 Published: 12 December 2021
Abstract
Background: Entomopathogenic nematodes (EPNs) are obligate lethal parasites of insects and are globally used as safe biocontrol agents against a wide range of insect pests. They occur in most agricultural soils all around the world. The insecticidal activity of two native EPNs Heterorhabditis bacteriophora (BA1) and Steinernema carpocapsae (BA2), isolated from the Egyptian soil was examined against eight different economic insect pests under laboratory conditions. These pests are the Greater wax moth larvae, Galleria mellonella; the cotton leafworm, Spodoptera littorallis; the cutworm, Agrotis ipsilon; the european corn borer, Osterinia nubilalis; the greater sugarcane borer, Sesamia critica; the apple tree borer, Zeuzera pyrina; the sugar beet fly, Pegomyia mixta and the tortoise beetle Cassida vittatta. Three nematode concentrations (100, 50, and 25 /ml water) of infective juveniles (IJs) were applied per insect. The test was conducted at 25± 2 °C and about 70± 2 % RH. Results: The median lethal concentration (LC50) of H. bacteriophora BA1– was almost– higher than that of S. capocapsae BA2 against the different tested insect pest larvae. The percentage of the cumulative mortality ranged between 46 and 100% in general, according to the tested concentration and/or the nematode species. The comparison between the mortality percentages caused by BA1 and BA2 nematodes to differently treated insect larvae revealed that BA1 was almost more virulent than BA2. Results showed that the lowest LC50 value was found to be 2.15 IJs, for H. bacteriophora BA1 isolate. Conclusions: The results showed that both indigenous EPNs isolates had a good impact in the management of the eight economic insect pests tested in this study had insecticidal properties and could be positively enrolled in integrated pest management programs against different insect pests.
Key Words: EPNs; Biocontrol; Cutworms; Borers; Sugar beet pests
References
| Abbott, W.S. (1925) A method for computing the effectiveness of an insecticide. J. Econ. Entomol. 18, 265-267. https://doi.org/10.1093/jee/18.2.265a |
||||
| Abdel-Razek A. S., Mona Hussein, Ibrahim Shehata (2018) Isolation and identification of indigenous entomopathogenic nematode (EPN) isolate from Egyptian fauna, Arch. Phytopathol. Plant Prot., 51:3-4, 197-206. https://doi.org/10.1080/03235408.2018.1445080 |
||||
| Amutha, V., Vengateswari, G., Shivakumar; M. S. (2020). Entomopathogenecity of nematode Panagrolaimus spp. (Rhabditida: Panagrolaimidae) against lepidopteran pest Spodoptera litura. International J. Pest Manag. DOI: 10.1080/09670874.2020.1776415 https://doi.org/10.1080/09670874.2020.1776415 |
||||
| Bedding, R.A., Molyneux, A.S. & Akhurst, R.J., 1983. Heterorhabditis spp., Neoaplectana spp. and Steinernema kraussei: interspecific and intraspecific differences in infectivity for insects. Exp. Parasitol. 55, 249-257. https://doi.org/10.1016/0014-4894(83)90019-X |
||||
| Burnell, A. M, Stock, S. P ( 2000) Heterorhabdits, Steinernema and their bacterial symbionts - lethal pathogens of insect. Nematology 2:3142. https://doi.org/10.1163/156854100508872 |
||||
| Duncan, DB (1965) Multiple range and multiple F-test. Biometrics, 11: 1-41. https://doi.org/10.2307/3001478 |
||||
| Ehlers, R.-U. (2005) Forum on Safety and Regulation. In: Nematodes as Biocontrol Agents. (Grewal, P. S., Ehlers, R.-U. and Shapiro-Ilan, D. I. eds.), CABI Publishing, Wallingford, 107-114. https://doi.org/10.1079/9780851990170.0107 |
||||
| El-Wakeil, N., Mona Hussein (2009) Field Performance of Entomopathogenic Nematodes and an Egg Parasitoid for Suppression of Corn Borers in Egypt. Arch. Phytopathol. Plant Prot., 42(3): 228-237. https://doi.org/10.1080/03235400600999422 |
||||
| Feaster, M.A., Steinkrauss, D.C (1999) Inundative biological control of Helicoverpa zea (Lepidoptera: Noctuidae) with the entomopathogenic nematode Steinernema riobravis (Rhabditida: Steinernematidae). Biol. Control 7, 38-43. https://doi.org/10.1006/bcon.1996.0061 |
||||
| Finney, D. J (1952) Probit Analysis. Cambridge, England, Cambridge University Press. | ||||
| Glazer, I, Navon, A (1990). Activity and persistence of entomoparasitic nematodes tested against Heliothis armigera (Lepidoptera: Noctuidae). J. Econ. Entomol. 83, 1795-1800. https://doi.org/10.1093/jee/83.5.1795 |
||||
| Glazer I, Galper S, Sharon E (1991) Virulence of the nematode (steinernematids and heterorhabditids)-bacteria (Xenorhabdus spp.) complex to the Egyptian cotton leafworm Spodoptera littoralis (Lepidoptera: Noctuidae). J. Invertebr. Pathol. 57, 94-100. https://doi.org/10.1016/0022-2011(91)90045-R |
||||
| Glazer I, Salame L, Goldenberg S, Blumberg D (1999) Susceptibility of Sap Beetles (Coleoptera: Nitidulidae) to Entomopathogenic Nematodes, Biocon. Sci. Technol., 9:2, 259-266, DOI: 10.1080/09583159929839 https://doi.org/10.1080/09583159929839 |
||||
| Griffin CT, Moore JF, Downes MJ (1991) Occurrence of insect parasitic nematodes (Steinernematidae, Hete-rorhabditidae) in the Republic of Ireland. Nematologica 37,92-100 https://doi.org/10.1163/187529291X00097 |
||||
| Hazir S, Kaya HK, Stock SP, Keskin N (2003) Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) for biological control of soil pests. Turk. J. Biol. 27, 181-202. | ||||
| Hominick WM (2002) Biogeography. In: Gaugler, R. (Ed.), entomopathogenic Nematology. CABI Publishing, Wallingford, UK, pp. 115- 144. https://doi.org/10.1079/9780851995670.0115 Hussein MA, El-Rahman RA, El-Boraey H, Hilmy M, Attya E (2018). The Impact of Cu Ion, Two Novel Schiff Base Ligands and their Copper (II) Complexes on the Biological Activity of the Entomopathogenic Nematodes. Microbial Bioactives, 1(2), 046-050 https://doi.org/10.25163/microbbioacts.12012A0512140918 |
||||
| Hussein Mona A., El-Mahdi Iman F S (2020) Artificial solid media for in-vitro mass production of two Egyptian nematodes. Bioscience Research 17(1):298-303 | ||||
| Hussein Mona A, El-Mahdi Iman F S (2019) Efficiency of three formulated entomopathogenic nematodes against the greenhouse onion thrips, Thrips tabaci under aquaculture system. J. Biopest., 12(1): 134-138. | ||||
| Hussein Mona A, Abou El- Soud A B (2006) Isolation and characterization of two Heterorhabditids and one Steinernematid nematodes from Egypt. Int. J. Nematol., 16(1):7-12. | ||||
| Hussein, Mona A.; Metwally, Hala M. S. & El-Raoaaf, M.A. (2015). Foliar Application of Native Bio-Formulated Entomopathogenic Nematodes against Diamondback Moth in Aquaponic Agriculture. Res. J. Pharma., Biologic. Chem. Sci., 6(6): 1030-1035. | ||||
| Iraki N, Salah N, Sansour MA, Segal D, Glazer I, Johnigk SA, Hussein MA, Ehlers R.-U (2000) Isolation and characterization of two entomopathogenic nematode strains, Heterorhabditis indica (Nematoda, Rhaditida), from the west Bank, Palastinian Territories. J. App.Entomol., 124: 375-380. https://doi.org/10.1046/j.1439-0418.2000.00450.x |
||||
| Metwally Hala M, Hafez Gehan A, Hussein Mona A, Hussein MA, Salem HA, MM Saleh (2012) Low Cost Artificial Diet for Rearing the Greater Wax Moth, Galleria mellonella L. (Lepidoptera: Pyralidae) as a Host for Entomopathogenic Nematodes. Egypt. J. Biol. Pest. Control, 22(1): 15-17 | ||||
| Navon A, Ascher, K (2000) Bioassays of entomopathogenic microbes and nematodes. CAB Publishing, Wallingford. https://doi.org/10.1079/9780851994222.0000 PMCid:PMC1550289 |
||||
| Nouh Gehan M, Hussein Mona A (2014) Virulence of Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) Produced in vitro Against Galleria mellonella (Lepidoptera: Pyralidae). Res. J. Pharma., Biologic. Chem. Sci., 5(3): 1385-93 | ||||
| Saleh MME, Draz KAA, Mansour MA, Mona A Hussein, Zawrah MFM (2009) Controlling the sugar beet weevil Cassida vittata with entomopathogenic nematodes. J. Pest Sci., 82: 289-294. https://doi.org/10.1007/s10340-009-0253-1 |
||||
| West RJ, Vrain TC (1997) Nematode control of black army cutworm (Lepidoptera: Noctuidae) under laboratory and field conditions. Canadian Entomol., 129, 229-239. https://doi.org/10.4039/Ent129229-2 |
||||
| White GF (1927) A method for obtaining infective nematode larvae from cultures. Science 66: 302-303. https://doi.org/10.1126/science.66.1709.302.b PMid:17749713 |
||||
| Williams CD, Dillon AB, Ennis D, Hennessy R, Griffin C (2015) Differential susceptibility of pine weevil, Hylobius abietis (Coleoptera: Curculionidae), larvae and pupae to entomopathogenic nematodes and death of adults infected as pupae. BioControl, 8 https://doi.org/10.1007/s10526-015-9658-3 |
||||
| Yuksel E, Canhilal R (2018) Evaluation of local isolates of entomopathogenic nematodes for the management of black cutworm, Agrotis ipsilon Hufnagel (Lepidoptera: Noctuidae). Egypt J Biol Pest Control 28, 82. https://doi.org/10.1186/s41938-018-0087-3 | ||||
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