Microbial Bioactives
MicroBio Pharmaceuticals and Pharmacology | Online ISSN 2209-2161
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RESEARCH ARTICLE   (Open Access)
Contents Vol 7 (1)

Antimicrobial activity, Phytochemical Screening of Crude Extracts, and Essential Constituents of Syzygium Aromaticum, Tymus Vulgaris and Eucalyptus Globulus on Selected Pathogens

Anubhav Dubey 1*, Mamta Kumari 1

+ Author Affiliations

Microbial Bioactives 7 (1) 1-5 https://doi.org/10.25163/microbbioacts.719791

Submitted: 19 April 2024 Revised: 18 June 2024  Published: 20 June 2024 

Abstract

Background: Plant materials are repositories of bioactive compounds with medicinal value, often utilized in traditional medicines for their therapeutic potential. This study aimed to evaluate the antimicrobial efficacy of extracts from Syzygium aromaticum, Thymus vulgaris, and Eucalyptus globulus against Salmonella typhi, Staphylococcus aureus, Escherichia coli, and Candida albicans. Methods: Plant extracts were obtained using soxhlet extraction with ethanol as the solvent. The extracts were qualitatively screened for the presence of alkaloids, saponins, flavonoids, and tannins. The residual concentrations of the plant extracts were determined to be 2.0 mg/mL for Syzygium aromaticum, 2.1 mg/mL for Thymus vulgaris, and 1.83 mg/mL for Eucalyptus globulus. The antimicrobial activity was assessed, and the minimum inhibitory concentration (MIC) for each extract against the tested microorganisms was determined. Results: All plant extracts contained saponins and flavonoids, while only extracts from Eucalyptus globulus, Thymus vulgaris, and Syzygium aromaticum contained tannins. Alkaloids were not present in the extracts. Staphylococcus aureus was susceptible only to Syzygium aromaticum extract. Salmonella typhi showed no sensitivity to any of the extracts. Escherichia coli was sensitive to all extracts, with the order of efficacy being Eucalyptus globulus > Thymus vulgaris > Syzygium aromaticum. Candida albicans was sensitive to Syzygium aromaticum and Thymus vulgaris extracts. The MIC for Syzygium aromaticum was 2.0 mg/mL for S. aureus, E. coli, and C. albicans. Thymus vulgaris exhibited MIC values of 0.021 mg/mL and 2.1 mg/mL for E. coli and C. albicans, respectively. Eucalyptus globulus had a MIC of 1.83 mg/mL for E. coli. Conclusion: The plant extracts from Syzygium aromaticum, Thymus vulgaris, and Eucalyptus globulus demonstrated antimicrobial activity against S. aureus, E. coli, and C. albicans. These findings suggest that these plant materials may be useful as natural remedies for managing infections caused by these susceptible microorganisms. Further research is warranted to explore their potential applications in clinical settings.

Keywords: Syzygium aromaticum, Antimicrobial, Tymus vulgaris, Pathogens, Eucalyptus globulus.

References

Albertyn, R., Berg, A., Numanoglu, A., & Rode, H. (2015). Traditional burn care in sub-Saharan Africa: a long history with wide acceptance. Burns : journal of the International Society for Burn Injuries, 41(2), 203–211. https://doi.org/10.1016/j.burns.2014.06.005

Bachir, R. G., & Benali, M. (2012). Antibacterial activity of the essential oils from the leaves of Eucalyptus globulus against Escherichia coli and Staphylococcus aureus. Asian Pacific journal of tropical biomedicine, 2(9), 739–742. https://doi.org/10.1016/S2221-1691(12)60220-2

Biswas, B., Rogers, K., McLaughlin, F., Daniels, D., & Yadav, A. (2013). Antimicrobial Activities of Leaf Extracts of Guava (Psidium guajava L.) on Two Gram-Negative and Gram-Positive Bacteria. International journal of microbiology, 2013, 746165. https://doi.org/10.1155/2013/746165

 Boruga, O., Jianu, C., Misca, C., Golet, I., Gruia, A. T., & Horhat, F. G. (2014). Thymus vulgaris essential oil: chemical composition and antimicrobial activity. Journal of medicine and life, 7 Spec No. 3(Spec Iss 3), 56–60.

Cortés-Rojas, D. F., de Souza, C. R., & Oliveira, W. P. (2014). Clove (Syzygium aromaticum): a precious spice. Asian Pacific journal of tropical biomedicine, 4(2), 90–96. https://doi.org/10.1016/S2221-1691(14)60215-X

Costa-de-Oliveira, S., & Rodrigues, A. G. (2020). Candida albicans Antifungal Resistance and Tolerance in Bloodstream Infections: The Triad Yeast-Host-Antifungal. Microorganisms, 8(2), 154. https://doi.org/10.3390/microorganisms8020154

Gurung, R. R., Maharjan, P., & Chhetri, G. G. (2020). Antibiotic resistance pattern of Staphylococcus aureus with reference to MRSA isolates from pediatric patients. Future science OA, 6(4), FSO464. https://doi.org/10.2144/fsoa-2019-0122

Hosseinzadeh, S., Jafarikukhdan, A., Hosseini, A. and Armand, R. (2015) The Application of Medicinal Plants in Traditional and Modern Medicine: A Review of Thymus vulgaris. International Journal of Clinical Medicine, 6, 635-642. http://dx.doi.org/10.4236/ijcm.2015.69084

Immanuel OM., Abu GO., Stanley HO. (2016). Inhibition of biogenic sulphide production and biocorrosion of carbon steel by sulphate reducing bacteria using Ocimum gratissimum essential oil. Journal of Biology & Biotechnology.10(2): 1-12.

Iwu, M.W., Duncan, A.R. and Okunji, C.O. (1999) New Antimicrobials of Plant Origin. In: Janick, J., Ed., Perspectives on New Crops and New Uses, ASHS Press, Alexandria, 457-462.

Jaiswal, Y., Liang, Z., & Zhao, Z. (2016). Botanical drugs in Ayurveda and Traditional Chinese Medicine. Journal of ethnopharmacology, 194, 245–259. https://doi.org/10.1016/j.jep.2016.06.052

Lade SN., Burle SS., Kosalge S.B, Bansode MN. (2022).Antimicrobial and Antioxidant activity of Hibiscus Sabdariffa. linn (Roselle) International Journal of Pharmacy Research & Technology. 12(1): doi: 10.31838/ijprt/12.01.04

Ocean, H. O., Eze, E. A., Eze, C. N., & Enebe, M. C. (2015). Drug resistance pattern of Salmonella and Shigella species isolated from selected hospitals in Anyigba, Kogi State, Nigeria. African Journal of Microbiology Research, 9(37), 2023-2036.

Okaba AE., Immanuel OM., Stow, KM., Oku IY. (2022).Evaluation of the antimicrobial activities of selected plant extracts and honey against clinical isolates. African Scientist. 23:(3) 30: 175-179.

Rojas, J. J., Ochoa, V. J., Ocampo, S. A., & Muñoz, J. F. (2006). Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: a possible alternative in the treatment of non-nosocomial infections. BMC complementary and alternative medicine, 6, 2. https://doi.org/10.1186/1472-6882-6-2

Ugboko HU., Nwinyi OC., Oranusi SU., Fatoki TH., Omonhinmin CA. (2020). Antimicrobial importance of medicinal plants in Nigeria. Sci. World J. 1 (1): 1-10, 10.1155/2020/7059323

Verpoorte D. R. (2017). New times for traditional medicine research. Journal of ethnopharmacology, 197, 1. https://doi.org/10.1016/j.jep.2017.01.018

Zhang, J., Onakpoya, I. J., Posadzki, P., & Eddouks, M. (2015). The safety of herbal medicine: from prejudice to evidence. Evidence-based complementary and alternative medicine : eCAM, 2015, 316706. https://doi.org/10.1155/2015/316706

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