Journal of Primeasia
Multidisciplinary research and review journal | Online ISSN 3064-9870
  1. You are here:
  2. Home
  3. Journals
  4. Biological Sciences
RESEARCH ARTICLE   (Open Access)
Contents Vol 2 (1)

Evaluation of Dye decolourization Potential of Laccase Producing TrichodermaharzianumDBS-1 and TrichodermaviridaeDBS-2

Ahmad M. 1*, Hussaini I.M. 2, Gide S.3, Anas G. 4

+ Author Affiliations

Journal of Primeasia 2 (1) 1-6 https://doi.org/10.25163/primeasia.2120215

Submitted: 19 March 2021 Revised: 12 May 2021  Published: 22 May 2021 

Abstract

Background: The presence of dye-containing effluents in water bodies and soil shows significant health risks to humans, plants, and animals. These adverse effects necessitate the search for safe, cost-effective, and environmentally friendly methods to remove dyes from these environments. This study aims to evaluate the dye decolourization potential of laccase-producing fungi, specifically Trichoderma harzianum DBS-1 and Trichoderma viridae DBS-2. Methods: Laccase-producing fungal isolates, T. harzianum DBS-1 and T. viridae DBS-2, were obtained from the laboratory at the Department of Microbiology, Ahmadu Bello University (ABU), Zaria, and stored on PDA slants. The isolates were screened for their ability to decolourize three dyes—Blue H3R, Yellow FG, and Red 3B—at concentrations of 50 ppm and 100 ppm. The fungi were inoculated into Malt Extract Broth containing each dye at the specified concentrations. Dye decolourization was assessed by measuring absorbance at 450 nm after 3, 6, and 9 days of incubation. Results: The results indicated that the fungal isolates could decolourize the dyes at varying rates. The highest decolourization was observed for Red 3B, with T. viridae DBS-2 achieving 71.32% decolourization at 50 ppm and 50.33% at 100 ppm after 9 days of incubation. Conversely, the lowest decolourization was recorded for Yellow FG by T. harzianum DBS-1, with 30.34% at 50 ppm and 20.87% at 100 ppm after 9 days. Blue H3R and Red 3B exhibited higher decolourization percentages compared to Yellow FG. Conclusion: The study demonstrates that laccase-producing isolates T. harzianum DBS-1 and T. viridae DBS-2 have significant potential for dye decolourization, particularly for Blue H3R and Red 3B dyes. These findings suggest that these fungal isolates could be employed as an effective, eco-friendly solution for the bioremediation of dye-contaminated environments.

Keywords: Dye, Laccase. Trichoderma, Decolourization, Environmental remediation.

References

Abdulredha, S. S. (2013). Production of laccase from Pleurotus spp. by solid state fermentation using agricultural wastes [Master’s thesis, College of Science, University of Baghdad].

Adiveppa, B. V., & Basappa, B. K. (2015). Isolation, screening and identification of laccase producing fungi. International Journal of Pharma and Bio Sciences, 6(3), 242-250.

Camila, C., Johnatan, V., Eduardo, E., Roselei, C. F., Marli, C., & Aldo, J. D. (2017). Synthetic dye decolorization by Marasmiellus palmivorus: Simultaneous cultivation and high laccase-crude broth treatment. Biocatalysis and Agricultural Biotechnology, 12, 314-322.

DeSouza-Ticlo, D., Tiwari, R., Sah, A. K., & Raghukumar, C. (2006). Enhanced production of laccase by a marine fungus during treatment of coloured effluents and synthetic dyes. Enzyme and Microbial Technology, 38, 504–511.

Eichlerova, I., Homolka, L., & Nerud, F. (2006). Synthetic dye decolorization capacity of white rot fungus Dichomitus squalens. Bioresource Technology, 97, 2153–2159.

Forootanfar, H., Faramarzi, M. A., Shahverdi, A. R., & Tabatabaei-Yazdi, M. (2011). Purification and biochemical characterization of extracellular laccase from the ascomycete Paraconiothyrium variabile. Bioresource Technology, 102, 1808–1814.

Forootanfar, H., Moezzi, A., Aghaie-Khozani, M., Mahmoudjanlou, Y., Ameri, A., Niknejad, F., & Faramarzi, M. A. (2012). Synthetic dye decolorization by three sources of fungal laccase. Iranian Journal of Environmental Health Science & Engineering, 9(27), 1-10.

Geethanjali, P. A., Gowtham, H. G., & Jayashankar, M. (2020). Optimization of culture conditions for hyper-production of laccase from an indigenous litter dwelling fungus Mucor circinelloides GL1. Environmental Sustainability, 3(4), 481-495.

Kowsalya, R. (2014). Laccase enzyme mediated biodegradation of textile effluents from Kanchipuram, Tamil Nadu, India by white rot fungi Pleurotus ostreatus. [Master’s thesis, Department of Biotechnology Interdisciplinary of Zoology/Biotechnology Faculty of Humanities and Sciences].

Olumide, D. O., Aliu, A., Ayodeji, O. A., & Akinniyi, A. O. (2013). Biodegradation of malachite green by extracellular laccase-producing Bacillus thuringiensis RUN1. Journal of Basic & Applied Sciences, 9, 43-549.

Perinbam, K., Bharath, B., Seeni, S., & Ravikumar, M. (2017). Lignocellulose degrading enzymes from fungi and their industrial applications. International Journal of Current Research and Review, 9(21), 1-12.

Pramanik, S., & Chaudhuri, S. (2018). Laccase activity and azo dye decolorization potential of Podoscypha elegans. Mycobiology, 46(1), 79-83.

Roseline, G. J., & Joel, J. G. (2014). Screening and molecular characterization of white rot fungi capable of laccase production and dye decolourization. International Journal of Life Science and Pharma Research, 4(2), 12-20.

Shekher, R., Sehgal, S., Kamthania, M., & Kumar, A. (2011). Laccase: Microbial sources, production, purification, and potential biotechnological applications. Enzyme Research, 2011, 1-12.

Zhuo, R., Ma, L., Fan, F., Gong, Y., Wan, X., Jiang, M., Zhang, X., & Yang, Y. (2011). Decolorization of different dyes by a newly isolated white-rot fungi strain Ganoderma sp. En3 and cloning and functional analysis of its laccase gene. Journal of Hazardous Materials, 192, 855–873.

Zubbair, N. A., Ajao, A. T., Adeyemo, E. O., & Adeniyi, D. O. (2018). Biotransformation and detoxification of reactive black dye by Ganoderma tsugae. African Journal of Environmental Science and Technology, 12(5), 158-171.

PDF
Abstract
Export Citation

View Dimensions


View Plumx


View Altmetric




Save
0
Citation
302
View

Share