Evaluation of Dye decolourization Potential of Laccase Producing TrichodermaharzianumDBS-1 and TrichodermaviridaeDBS-2
Ahmad M. 1*, Hussaini I.M. 2, Gide S.3, Anas G. 4
Journal of Primeasia 2(1) 1-6 https://doi.org/10.25163/primeasia.2120215
Submitted: 19 March 2021 Revised: 12 May 2021 Published: 22 May 2021
Abstract
Background: The presence of dye-containing effluents in water bodies and soil shows significant health risks to humans, plants, and animals. These adverse effects necessitate the search for safe, cost-effective, and environmentally friendly methods to remove dyes from these environments. This study aims to evaluate the dye decolourization potential of laccase-producing fungi, specifically Trichoderma harzianum DBS-1 and Trichoderma viridae DBS-2. Methods: Laccase-producing fungal isolates, T. harzianum DBS-1 and T. viridae DBS-2, were obtained from the laboratory at the Department of Microbiology, Ahmadu Bello University (ABU), Zaria, and stored on PDA slants. The isolates were screened for their ability to decolourize three dyes—Blue H3R, Yellow FG, and Red 3B—at concentrations of 50 ppm and 100 ppm. The fungi were inoculated into Malt Extract Broth containing each dye at the specified concentrations. Dye decolourization was assessed by measuring absorbance at 450 nm after 3, 6, and 9 days of incubation. Results: The results indicated that the fungal isolates could decolourize the dyes at varying rates. The highest decolourization was observed for Red 3B, with T. viridae DBS-2 achieving 71.32% decolourization at 50 ppm and 50.33% at 100 ppm after 9 days of incubation. Conversely, the lowest decolourization was recorded for Yellow FG by T. harzianum DBS-1, with 30.34% at 50 ppm and 20.87% at 100 ppm after 9 days. Blue H3R and Red 3B exhibited higher decolourization percentages compared to Yellow FG. Conclusion: The study demonstrates that laccase-producing isolates T. harzianum DBS-1 and T. viridae DBS-2 have significant potential for dye decolourization, particularly for Blue H3R and Red 3B dyes. These findings suggest that these fungal isolates could be employed as an effective, eco-friendly solution for the bioremediation of dye-contaminated environments.
Keywords: Dye, Laccase. Trichoderma, Decolourization, Environmental remediation.
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