Isolation, Identification, and Antibiotic Susceptibility Analysis of Bacterial Pathogens in Suspected Urinary Tract Infection Cases at a Tertiary Medical Center in Dhaka, Bangladesh

Md. Robeul Islam¹, Avijit Banik¹, Dr. Kumkum Rahman Mouree¹, Suvamoy Datta^1*

Identifies prevalent UTI pathogens and effective treatments, aiding clinicians in managing infections amidst rising antibiotic resistance challenges in Bangladesh.

Abstract

Objective: A urinary tract infection (UTI) is a bacterial infection of the urinary tract. The lower urinary tract, bladder, and urethra are the most often infected areas. Women are more likely than men to have a bacterial infection. The research aimed to determine the causative agent of UTI in patients and see how they responded to standard treatments. Methods: A total of 435 urine samples were examined using the culture technique. The samples were streaked evenly on blood agar and MacConkey for morphological characteristics of the colony on media to identify the presumptive bacterium. Gram staining and routine biochemical assays were also used to confirm the findings. The disk diffusion technique was employed to assess susceptibility to 12 different antibiotics. Results: The most prevalent uropathogenic in both genders and age groups was E. coli (44%), Staphylococcus aureus (21%), Klebsiella spp. (13%) and Proteus spp. (12%), Enterobacter spp. (10%). Overall, the uropathogenic were highest susceptible to Meropenem (82.2%), Amikacin (63.6%), and Cefixime (59.8%) were the most successful medications for the treatment of UTI, the highest resistance to Azithromycin (84.2%), Gentamycin (75.6%) and Nalidixic acid (64.4%) were the least effective. Conclusion: The present study can be helpful for clinicians in finding proper drugs in developing countries like Bangladesh, where the multi-drug resistance problem has just complicated the treatment of UTIs.

Keywords: Urinary tract infection, Causative agents, Antibiotic susceptibility, Escherichia coli, Treatment efficacy.

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