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Contents Vol 7 (1)

Moringa oleifera Extraction, Isolation, Quantification and Its Application in Functional Foods: A review

Rolla Al-Shalabi 1, Nozlena Abdul Samad 1*

+ Author Affiliations

Australian Herbal Insight 7 (1) 1-10 https://doi.org/10.25163/ahi.719958

Submitted: 28 February 2024 Revised: 13 May 2024  Published: 15 May 2024 

Abstract

Background: M. oleifera, a rapidly growing and drought-resistant tree belonging to the Moringaceae family, originates from the Indian subcontinent. Commonly referred to as Moringa, drumstick tree, horseradish tree, or ben oil tree, it is cultivated worldwide for its nutrient-dense seed pods and leaves, which serve as vegetables and are utilized in traditional medicinal practices. Furthermore, Moringa is admitted for its capacity to purify water. The rising interest in natural products has heightened the significance of Moringa, owing to its nutritional and therapeutic properties, thereby establishing it as an essential element in health promotion and disease prevention. Objective: This article intends to explore the diverse extraction techniques employed for the isolation of bioactive compounds from M. oleifera, with a focus on the effectiveness and specificity of various methodologies for understanding Moringa's role in nutritional health and disease prevention. Methodology: This study examines various extraction techniques for M. oleifera, such as ultrasound-assisted extraction, microwave-assisted extraction, aqueous two-phase system, and pressurized hot water extraction. Results: The findings indicate that each extraction technique possesses distinct benefits, with high-performance liquid chromatography playing a vital role in the precise identification and quantification of various compounds. The combination of advanced extraction techniques and high-performance liquid chromatography significantly improves the effectiveness of isolating the essential nutrients and bioactive compounds found in Moringa components, such as carbohydrates, proteins, vitamins, and essential minerals. Conclusion: M. oleifera is recognized as a significant source of both nutritional and therapeutic compounds. The application of current extraction techniques, along with high-performance liquid chromatography, enables the efficient extraction of its health-enhancing constituents, highlighting its potential role in fostering general health and aiding disease prevention. As the demand for natural products increases, Moringa remains an essential focus in the fields of nutritional and medicinal research.

Keywords: Extraction methods, Functional foods, Isolation techniques, M. oleifera, Quantification.

References

Abdull Razis, A. F., Ibrahim, M. D., & Kntayya, S. B. (2014). Health benefits of Moringa oleifera. Asian Pacific Journal of Cancer Prevention, 15(20), 8571–8576.

Albarri, R., Toprakçi, I., Kurtulbas, E., & Sahin, S. (2021). Estimation of diffusion and mass transfer coefficients for the microwave-assisted extraction of bioactive substances from Moringa oleifera leaves. Biomass Conversion and Biorefinery, 1–8.

Altemimi, A., Lakhssassi, N., Baharlouei, A., Watson, D. G., & Lightfoot, D. A. (2017). Phytochemicals: Extraction, isolation, and identification of bioactive compounds from plant extracts. Plants, 6(4), 42.

Azwanida, N. N. (2015). A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants, 4(196), 412–2167.

Buddin, M., Rithuan, M. Z. A., Surni, M. S. A., Jamal, N. H. M., & Faiznur, M. F. (2018). Ultrasonic assisted extraction (UAE) of Moringa oleifera Seed Oil: Kinetic study. ASM Sci. J, 11(3), 158–166.

Chokwe, R. C., Dube, S., & Nindi, M. M. (2020). Development of an HPLC-DAD method for the quantification of ten compounds from Moringa oleifera Lam. and its application in quality control of commercial products. Molecules, 25(19), 4451.

Cozzolino, D. (2015). Infrared spectroscopy as a versatile analytical tool for the quantitative determination of antioxidants in agricultural products, foods and plants. Antioxidants, 4(3), 482–497.

de Brito Cardoso, G., Mourão, T., Pereira, F. M., Freire, M. G., Fricks, A. T., Soares, C. M. F., & Lima, Á. S. (2013). Aqueous two-phase systems based on acetonitrile and carbohydrates and their application to the extraction of vanillin. Separation and Purification Technology, 104, 106–113.

Dhanani, T., Shah, S., Gajbhiye, N. A., & Kumar, S. (2017). Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arabian Journal of Chemistry, 10, S1193–S1199.

Djande, C. Y. H., Piater, L. A., Steenkamp, P. A., Madala, N. E., & Dubery, I. A. (2018). Differential extraction of phytochemicals from the multipurpose tree, Moringa oleifera, using green extraction solvents. South African Journal of Botany, 115, 81–89.

Gbashi, S., Njobeh, P., Steenkamp, P., & Madala, N. (2017). Pressurized hot water extraction and chemometric fingerprinting of flavonoids from Bidens pilosa by UPLC-tandem mass spectrometry. CyTA-Journal of Food, 15(2), 171–180.

Golebiewska, E., Kalinowska, M., & Yildiz, G. (2022). Sustainable use of apple pomace (AP) in different industrial sectors. Materials, 15(5), 1788.

Gopalakrishnan, L., Doriya, K., & Kumar, D. S. (2016). Moringa oleifera: A review on nutritive importance and its medicinal application. Food Science and Human Wellness, 5(2), 49–56.

Gu, F., Gao, J., Xiao, J., Chen, Q., Ruan, H., & He, G. (2012). Efficient methods of purification of α-galactosidase from Aspergillus niger: aqueous two-phase system versus three-phase partitioning. Rom Biotechnol Lett, 17, 7853–7862.

Heng, M. Y., Tan, S. N., Yong, J. W. H., & Ong, E. S. (2013). Emerging green technologies for the chemical standardization of botanicals and herbal preparations. TrAC Trends in Analytical Chemistry, 50, 1–10.

Iqbal, M., Tao, Y., Xie, S., Zhu, Y., Chen, D., Wang, X., Huang, L., Peng, D., Sattar, A., & Shabbir, M. A. B. (2016). Aqueous two-phase system (ATPS): an overview and advances in its applications. Biological Procedures Online, 18, 1–18.

Joshi, P., & Varma, K. (2017). Preparation of Value Added Products from Dehydrated Drumstick Leaves. Online Int. Interdiscip. J, 7, 41–48.

Kazemi, M., Karim, R., Mirhosseini, H., & Hamid, A. A. (2016). Optimization of pulsed ultrasound-assisted technique for extraction of phenolics from pomegranate peel of Malas variety: Punicalagin and hydroxybenzoic acids. Food Chemistry, 206, 156–166.

Kholif, A. E., Gouda, G. A., Galyean, M. L., Anele, U. Y., & Morsy, T. A. (2019). Extract of Moringa oleifera leaves increases milk production and enhances milk fatty acid profile of Nubian goats. Agroforestry Systems, 93, 1877–1886.

Kholif, A. E., Morsy, T. A., Gouda, G. A., Anele, U. Y., & Galyean, M. L. (2016). Effect of feeding diets with processed Moringa oleifera meal as protein source in lactating Anglo-Nubian goats. Animal Feed Science and Technology, 217, 45–55.

Khoza, B. S., Chimuka, L., Mukwevho, E., Steenkamp, P. A., & Madala, N. E. (2014). The Effect of Temperature on Pressurised Hot Water Extraction of Pharmacologically Important Metabolites as Analysed by UPLC-qTOF-MS and PCA. Evidence-Based Complementary and Alternative Medicine, 2014(1), 914759.

Liang, X., & Fan, Q. (2013). Application of sub-critical water extraction in pharmaceutical industry. Journal of Materials Science and Chemical Engineering, 1(05), 1.

Liu, F., Ong, E. S., & Li, S. F. Y. (2013). A green and effective approach for characterisation and quality control of Chrysanthemum by pressurized hot water extraction in combination with HPLC with UV absorbance detection. Food Chemistry, 141(3), 1807–1813.

Liu, X., Mu, T., Sun, H., Zhang, M., & Chen, J. (2013). Optimisation of aqueous two-phase extraction of anthocyanins from purple sweet potatoes by response surface methodology. Food Chemistry, 141(3), 3034–3041.

Lu, C., Wang, H., Lv, W., Ma, C., Lou, Z., Xie, J., & Liu, B. (2012). Ionic liquid-based ultrasonic/microwave-assisted extraction combined with UPLC–MS–MS for the determination of tannins in Galla chinensis. Natural Product Research, 26(19), 1842–1847.

MS, U., Ferdosh, S., Haque Akanda, M. J., Ghafoor, K., AH, R., Ali, M. E., Kamaruzzaman, B. Y., MB, F., Shaarani, S., & Islam Sarker, M. Z. (2018). Techniques for the extraction of phytosterols and their benefits in human health: A review. Separation Science and Technology, 53(14), 2206–2223.

Panzella, L., Moccia, F., Nasti, R., Marzorati, S., Verotta, L., & Napolitano, A. (2020). Bioactive phenolic compounds from agri-food wastes: An update on green and sustainable extraction methodologies. Frontiers in Nutrition, 7, 60.

Petigny, L., Périno-Issartier, S., Wajsman, J., & Chemat, F. (2013). Batch and continuous ultrasound assisted extraction of boldo leaves (Peumus boldus Mol.). International Journal of Molecular Sciences, 14(3), 5750–5764.

Plaza, M., Abrahamsson, V., & Turner, C. (2013). Extraction and neoformation of antioxidant compounds by pressurized hot water extraction from apple byproducts. Journal of Agricultural and Food Chemistry, 61(23), 5500–5510.

Plaza, M., & Turner, C. (2015). Pressurized hot water extraction of bioactives. TrAC Trends in Analytical Chemistry, 71, 39–54.

Poobalan, K., Lim, V., Kamal, N. N. S. N. M., Yusoff, N. A., Khor, K. Z., & Samad, N. A. (2018). Effects of ultrasound assisted sequential extraction (UASE) of Moringa oleifera leaves extract on MCF 7 human breast cell line. Malaysian Journal of Medicine & Health Sciences, 14.

Prasetyaningrum, A., Rokhati, N., Dharmawan, Y., & Prinanda, G. R. (2021). Comparison study for extraction of bioactive flavonoids from moringa oleifera, apple, onion, and ascorbic acid (orange) by using microwave-assisted, ultrasound-assisted and maceration methods. IOP Conference Series: Materials Science and Engineering, 1053(1), 12123.

Rehman, M. U., Khan, F., & Niaz, K. (2020). Introduction to natural products analysis. In Recent advances in natural products analysis (pp. 3–15). Elsevier.

Rodríguez-Pérez, C., Quirantes-Piné, R., Fernández-Gutiérrez, A., & Segura-Carretero, A. (2015). Optimization of extraction method to obtain a phenolic compounds-rich extract from Moringa oleifera Lam leaves. Industrial Crops and Products, 66, 246–254.

Ruiz-Ruiz, F., Benavides, J., Aguilar, O., & Rito-Palomares, M. (2012). Aqueous two-phase affinity partitioning systems: current applications and trends. Journal of Chromatography A, 1244, 1–13.

Sahay, S., Yadav, U., & Srinivasamurthy, S. (2017). Potential of Moringa oleifera as a functional food ingredient: A review. Magnesium (g/Kg), 8(9.06), 4–90.

Sengev, A. I., Abu, J. O., & Gernah, D. I. (2013). Effect of Moringa oleifera leaf powder supplementation on some quality characteristics of wheat bread. Food and Nutrition Sciences, 4(3), 270.

Siddiqui, M. R., AlOthman, Z. A., & Rahman, N. (2017). Analytical techniques in pharmaceutical analysis: A review. Arabian Journal of Chemistry, 10, S1409–S1421.

Singh, Y., & Prasad, K. (2013). Moringa oleifera leaf as functional food powder. Charact Uses, 4, 317–324.

Taamalli, A., Arráez-Román, D., Barrajón-Catalán, E., Ruiz-Torres, V., Pérez-Sánchez, A., Herrero, M., Ibañez, E., Micol, V., Zarrouk, M., & Segura-Carretero, A. (2012). Use of advanced techniques for the extraction of phenolic compounds from Tunisian olive leaves: Phenolic composition and cytotoxicity against human breast cancer cells. Food and Chemical Toxicology, 50(6), 1817–1825.

Thapa, K., Poudel, M., & Adhikari, P. (2019). Moringa oleifera: A review article on nutritional properties and its prospect in the context of Nepal. Acta Sci. Agric, 3(11), 47–54.

Zhang, D.-Y., Zu, Y.-G., Fu, Y.-J., Wang, W., Zhang, L., Luo, M., Mu, F.-S., Yao, X.-H., & Duan, M.-H. (2013). Aqueous two-phase extraction and enrichment of two main flavonoids from pigeon pea roots and the antioxidant activity. Separation and Purification Technology, 102, 26–33.

 

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