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RESEARCH ARTICLE   (Open Access)
Contents Vol 6 (1)

Cytotoxic and Antioxidant Activities, and Phytochemical Analysis of Methanol Extract of Euphorbia cotinifolia

Muhammad Rashad 1,2*, Malik Saadullah 2

+ Author Affiliations

Australian Herbal Insight 6 (1) 1-7 https://doi.org/10.25163/ahi.617332

Submitted: 20 January 2023 Revised: 10 April 2023  Published: 20 April 2023 

Abstract

Background: Plants contain complex and structurally different phytochemicals. The popularity of herbal medicinal products for a variety of disorders in recent years has prompted researchers to study the therapeutic activities of several medicinal plants. The current study was carried out to investigate the cytotoxic and antioxidant activity, and various phytochemicals present in Euphorbia cotinifolia. Material & Methods: Crushed whole plant was used and extraction was performed by using methanol. Different physicochemical and identification tests were performed to determine the presence of phytochemicals, folin-ciocalteu principle for phenolic compounds and standard quercetin method was used to determine total flavonoid contents. FTIR for functional groups, DPPH method for antioxidant activity and MTT assay was used to evaluate the cytotoxic activity. Results: Methanol extract contained a significant amount of phenolics (62.81 ± 1.94 mg GAE/g) and flavonoids (33.75 ± 1.98 mg QE/g) contents. FTIR spectrum stipulated the occupancy of carboxylic acids, alkenes, and aromatic groups. Antioxidant activity was 82 % at 1000 µg/ml having IC50 value 18.61±0.25 µg/ml as compared to standard (ascorbic acid). Cytotoxic activity showed percent inhibition 69.50 % and IC50 value 17.15 ±0.3 against standard (doxorubicin). Conclusion: It was concluded that Euphorbia cotinifolia contains phenolic and flavonoids compounds that are responsible for antioxidant and cytotoxic activity. It can be used for isolation of novel compound responsible for anticancer activity and other pharmacological actions.

Keywords: Euphorbia cotinifolia, Antioxidant, Total Phenolic contents, Total Flavonoids contents, cytotoxic activity.

References

Aktumsek, A., et al. (2013). Antioxidant potentials and anticholinesterase activities of methanolic and aqueous extracts of three endemic Centaurea L. species. Food and Chemical Toxicology, 55, 290-296.

Allkin, B. (2017). Useful plants–medicines: At least 28,187 plant species are currently recorded as being of medicinal use.

Anand, U., et al. (2019). A comprehensive review on medicinal plants as antimicrobial therapeutics: Potential avenues of biocompatible drug discovery. Metabolites, 9(11), 258.

Ayesha, S., Premakumari, K., & Roukiya, S. (2010). Antioxidant activity and estimation of total phenolic content of Muntingia Calabura by colorimetry. International Journal of ChemTech Research, 2(1), 205-208.

Aziz, M. A., et al. (2018). Traditional uses of medicinal plants used by Indigenous communities for veterinary practices at Bajaur Agency, Pakistan. Journal of Ethnobiology and Ethnomedicine, 14(1), 11.

Bates, J. (1976). Fourier transform infrared spectroscopy. Science, 191(4222), 31-37.

Baylac, S., & Racine, P. (2003). Inhibition of 5-lipoxygenase by essential oils and other natural fragrant extracts. International Journal of Aromatherapy, 13(2-3), 138-142.

Brain, K. R., & Turner, T. D. (1975). The practical evaluation of phytopharmaceuticals. Vol. 1. Wright-Scientechnica Bristol.

CI, K. C., & Indira, G. (2016). Quantitative estimation of total phenolic, flavonoids, tannin and chlorophyll content of leaves of Strobilanthes Kunthiana (Neelakurinji). Journal of Medicinal Plants, 4, 282-286.

Ekor, M. (2014). The growing use of herbal medicines: Issues relating to adverse reactions and challenges in monitoring safety. Frontiers in Pharmacology, 4, 177.

Ernst, E. (2005). The efficacy of herbal medicine – an overview. Fundamental & Clinical Pharmacology, 19(4), 405-409.

Ernst, E., & Pittler, M. (2002). Herbal medicine. The Medical Clinics of North America, 86(1), 149-161.

Faix, O. (1992). Fourier transform infrared spectroscopy. In Methods in Lignin Chemistry (pp. 83-109). Springer.

Hossain, H., et al. (2016). HPLC profiling, antioxidant and in vivo anti-inflammatory activity of the ethanol extract of Syzygium jambos available in Bangladesh. BMC Research Notes, 9(1), 1-8.

Ismail, A., Marjan, Z. M., & Foong, C. W. (2004). Total antioxidant activity and phenolic content in selected vegetables. Food Chemistry, 87(4), 581-586.

Jayalakshmi, B., Raveesha, K. A., & Amruthesh, K. N. (2021). Isolation and characterization of bioactive compounds from Euphorbia cotinifolia. Future Journal of Pharmaceutical Sciences, 7(1), 9.

Kalita, P., et al. (2013). Estimation of total flavonoids content (TFC) and antioxidant activities of methanolic whole plant extract of Biophytum sensitivum Linn. Journal of Drug Delivery and Therapeutics, 3(4), 33-37.

Kaur, N., Bhatia, A., & Sidhu, S. (2017). Antimicrobial activity and phytochemical screening of extracts of Euphorbia cotinifolia, 2, 2455-698X.

Kupina, S., et al. (2018). Determination of Total Phenolic Content Using the Folin-C Assay: Single-Laboratory Validation, First Action 2017.13. Journal of AOAC International, 101(5), 1466-1472.

Madaan, R., et al. (2011). Estimation of Total Phenols and Flavonoids in Extracts of Actaea spicata Roots and Antioxidant Activity Studies. Indian Journal of Pharmaceutical Sciences, 73(6), 666-669.

Maregesi, S. M., et al. (2007). Ethnopharmacological survey of the Bunda district, Tanzania: Plants used to treat infectious diseases. Journal of Ethnopharmacology, 113(3), 457-470.

Ogbole, O. O., Segun, P. A., & Adeniji, A. J. (2017). In vitro cytotoxic activity of medicinal plants from Nigeria ethnomedicine on Rhabdomyosarcoma cancer cell line and HPLC analysis of active extracts. BMC Complementary and Alternative Medicine, 17(1), 494.

Salehi, B., et al. (2019). Euphorbia-Derived Natural Products with Potential for Use in Health Maintenance. Biomolecules, 9(8), 337.

Sarah, Q. S., Anny, F. C., & Mir, M. (2017). Brine shrimp lethality assay. Bangladesh Journal of Pharmacology, 12(2), 186-189.

Sharma, O. P., & Bhat, T. K. (2009). DPPH antioxidant assay revisited. Food Chemistry, 113(4), 1202-1205.

Sofowora, A., Ogunbodede, E., & Onayade, A. (2013). The role and place of medicinal plants in the strategies for disease prevention. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, 10(5), 210-229.

Tadesse, S., et al. (2016). Preliminary phytochemical screening of Euphorbia Cotinifolia L. (Family: Euphorbiacae) by using different solvent extracts. World Journal of Pharmacy and Pharmaceutical Sciences, 5, 1176-1183.

Van Bavel, J. (2013). The world population explosion: Causes, backgrounds and projections for the future. Facts, Views & Vision in ObGyn, 5(4), 281-291.

Vijayarathna, S., & Sasidharan, S. (2012). Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines. Asian Pacific Journal of Tropical Biomedicine, 2(10), 826-829.

Wachtel-Galor, S., & Benzie, I. F. (2011). Herbal medicine: Biomolecular and clinical aspects. Boca Raton: CRC Press.

Waghulde, S., Kale, M. K., & Patil, V. (2019). Brine Shrimp Lethality Assay of the Aqueous and Ethanolic Extracts of the Selected Species of Medicinal Plants. In Multidisciplinary Digital Publishing Institute Proceedings.

Yener, I., et al. (2018). A detailed study on chemical and biological profile of nine Euphorbia species from Turkey with chemometric approach: Remarkable cytotoxicity of E. fistulasa and promising tannic acid content of E. eriophora. Industrial Crops and Products, 123, 442-453.

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