Lactoferrin Supplementation Induced Awassi Lambs Production with High Immunity and Iron Levels

Emad Gh.AL-Abbasy ^1*, Adnan Gh. Jalal ^1* 

Lactoferrin supplementation might increase immune function and iron levels in Awassi lambs, improving health and productivity.

Abstract

Background: The Food and Agriculture Organization (FAO) reports indicate an increasing demand for animal products in the Near East and North Africa, surpassing production rates. This necessitates a focus on enhancing animal production, particularly sheep breeding, which is prevalent in the region. Newborn lamb morbidity and mortality are significant economic losses for sheep farms, attributed to deficiencies in immunoglobulins at birth. Colostrum intake is crucial for newborn immunity and growth, containing vital immunoglobulins and antibodies. Lactoferrin, a key component in colostrum, plays a role in boosting the immune system and nutrient absorption. Methods: This study was conducted at the Department of Animal Production, Faculty of Agriculture, University of Tikrit, from November 1, 2023, to February 1, 2024. Fifteen Awassi lambs were divided into three groups: control (no lactoferrin), 1 g/day lactoferrin, and 3 g/day lactoferrin. Blood samples were collected at 30, 60, and 90 days of age to measure concentrations of immunoglobulins (IgG, IgM, IgA), iron, transferrin, and transferrin saturation. The analysis was performed using radial immunodiffusion, Cobas C6000 automatic analyzer, and Alisa (Stat Fax 2400). Results: At 30 days, the lambs receiving lactoferrin showed significantly higher IgG levels compared to the control group. IgG concentrations were 13.20 ± 0.1 mg/ml (3 g lactoferrin) and 10.40 ± 0.3 mg/ml (1 g lactoferrin), compared to 9.30 ± 2.1 mg/ml in the control group. No significant differences were observed in IgM and IgA levels among the groups. Iron concentrations showed no significant differences at 30 days, but transferrin levels were significantly lower in the 3 g lactoferrin group (2.10 ± 0.30 mg/ml) compared to the control (2.90 ± 0.12 mg/ml) and 1 g lactoferrin (2.80 ± 0.16 mg/ml) groups. At 60 and 90 days, iron concentrations were significantly higher in the lactoferrin groups compared to the control. Conclusion: The administration of lactoferrin significantly enhances the concentration of immunoglobulins and iron in the blood of newborn Awassi lambs, indicating improved immune function and nutrient absorption.

Keywords: Lactoferrin, Immunoglobulins, Iron concentration, Transferrin, Awassi lambs

References

Abdulqader, A. T., Al-Sammarie, A. M. Y., & Mustafa, M. A. (2022, May). A comparative environmental study of aqueous extracts of ginger and grapes to protect hepatocytes in Albino rabbits and a comparison of extracts in preserving Awassi lamb meat from oxidation. In IOP Conference Series: Earth and Environmental Science (Vol. 1029, No. 1, p. 012001). IOP Publishing.‏

Ali, S. H., Armeet, H. S., Mustafa, M. A., & Ahmed, M. T. (2022, November). Complete blood count for COVID-19 patients based on age and gender. In AIP Conference Proceedings (Vol. 2394, No. 1). AIP Publishing.‏

ALkudsy, H. N., Waleed, A., & Khalid. (2018). Association of lactoferrin with some immunological and blood traits of Holstein calves in the middle of Iraq. Journal of Research in Ecology, 6(2), 1778.

Al-Obaidi, M. M. J., Suhaili, Z., & Desa, M. N. (2018). Genotyping approaches for identification and characterization of Staphylococcus aureus. IntechOpen: London, 25(5), 212-225.

Brujeni, G. N., Jani, S. S., Alidadi, N., Tabatabaei, S., Sharifi, H., & Mohri, M. (2010). Passive immune transfer in fat-tailed sheep: Evaluation with different methods. Small Ruminant Research, 90(1-3), 146-149.

Comstock, S. S., Reznikov, E. A., Contractor, N., & Donovan, S. M. (2014). Dietary Bovine Lactoferrin Alters Mucosal and Systemic Immune Cell Responses in Neonatal Piglets. The Journal of Nutrition, 144(4), 525-532.

Gökçe, E., Kirmizigül, A. H., Erdogan, H. M., & ÇItIl, M. (2013). Risk factors associated with passive immunity, health, birth weight, and growth.

Hsu, C. Y., Mustafa, M. A., Yadav, A., Batoo, K. M., Kaur, M., Hussain, S., ... & Nai, L. (2024). N2 reduction to NH3 on surfaces of Co-Al18P18, Ni-Al21N21, Fe-B24N24, Mn-B27P27, Ti-C60, and Cu-Si72 catalysts. Journal of Molecular Modeling, 30(3), 1-11.‏

Hurley, W. L. (2003). Immunoglobulins in mammary secretions. In Advanced Dairy Chemistry—1 Proteins (pp. 421-447). Springer, Boston, MA.

Kadham, S. M., Mustafa, M. A., Abbass, N. K., & Karupusamy, S. (2024). IoT and artificial intelligence–based fuzzy-integral N-transform for sustainable groundwater management. Applied Geomatics, 16(1), 1-8.‏

Kadham, S. M., Mustafa, M. A., Abbass, N. K., & Karupusamy, S. (2023). Comparison between of fuzzy partial H-transform and fuzzy partial Laplace transform in x-ray images processing of acute interstitial pneumonia. International Journal of System Assurance Engineering and Management, 1-9.‏

Killip, S., Bennett, J. M., & Chambers, M. D. (2007). Iron deficiency anemia. American Family Physician.

Lu, Z. F., Hsu, C. Y., Younis, N. K., Mustafa, M. A., Matveeva, E. A., Al-Juboory, Y. H. O., ... & Abdulraheem, M. N. (2024). Exploring the significance of microbiota metabolites in rheumatoid arthritis: uncovering their contribution from disease development to biomarker potential. APMIS.‏

Macedo, M. F., & Sousa, M. D. (2008). Transferrin and the transferrin receptor: of magic bullets and other concerns. Inflammation & Allergy-Drug Targets, 7(1), 41-52.

Mahmoud, Z. H., Ajaj, Y., Hussein, A. M., Al-Salman, H. N. K., Mustafa, M. A., Kadhum, E. H., ... & Kianfar, E. (2024). CdIn2Se4@ chitosan heterojunction nanocomposite with ultrahigh photocatalytic activity under sunlight driven photodegradation of organic pollutants. International Journal of Biological Macromolecules, 267, 131465.‏

Meri, M. A., Ibrahim, M. D., Al-Hakeem, A. H., & Mustafa, M. A. (2023). Procalcitonin and NLR Measurements in COVID-19 Patients. Latin American Journal of Pharmacy, 220-223.‏

Mustafa, M. A., Kadham, S. M., Abbass, N. K., Karupusamy, S., Jasim, H. Y., Alreda, B. A., ... & Ahmed, M. T. (2024). A novel fuzzy M-transform technique for sustainable groundwater level prediction. Applied Geomatics, 16(1), 9-15.‏

Mustafa, M. A., Raja, S., Asadi, L. A. A., Jamadon, N. H., Rajeswari, N., & Kumar, A. P. (2023). A Decision-Making Carbon Reinforced Material Selection Model for Composite Polymers in Pipeline Applications. Advances in Polymer Technology, 2023(1), 6344193.‏

Nakanishi, T., Kuragano, T., Nanami, M., Otaki, Y., Nonoguchi, H., & Hasuike, Y. (2010). Importance of ferritin for optimizing anemia therapy in chronic kidney disease. American Journal of Nephrology, 32(5), 439-446.

Ochoa, T. J., Zegarra, J., Cam, L. L., Llanos, R., Pezo, A., Cruz, K., Zea-Vera, A., Cárcamo, C., Campos, M., & Bellomo, S. (2015). Randomized controlled trial of lactoferrin for prevention of sepsis in Peruvian neonates < 2500 grams. The Pediatric Infectious Disease Journal, 34(6), 571.

Paesano, R., Berlutti, F., Pietropaoli, M., Goolsbee, W., Pacifici, E., & Valenti, P. (2010). Lactoferrin efficacy versus ferrous sulfate in curing iron disorders in pregnant and non-pregnant women. International Journal of Immunopathology and Pharmacology, 23(2), 577-587.

Prenner, M. L., Prgomet, C., Sauerwein, H., Pfaffl, M. W., Broz, J., & Schwarz, F. J. (2007). Effects of lactoferrin feeding on growth, feed intake, and health of calves. Archives of Animal Nutrition, 61(1), 20-30.

Prgomet, C., Prenner, M. L., Schwarz, F. J., & Pfaffl, M. W. (2007). Effect of lactoferrin on selected immune system parameters and the gastrointestinal morphology in growing calves. Journal of Animal Physiology and Animal Nutrition, 91(3-4), 109-119.

Rezk, M., Kandil, M., Dawood, R., Shaheen, A. E., & Allam, A. (2015). Oral lactoferrin versus ferrous sulphate and ferrous fumerate for the treatment of iron deficiency anemia during pregnancy. Journal of Advanced Nutrition and Human Metabolism, 1.

Saadh, M. J., Avecilla, F. R. B., Mustafa, M. A., Kumar, A., Kaur, I., Alawayde, Y. M., ... & Elmasry, Y. (2024). The promising role of doped h-BANDs for solar cells application: A DFT study. Journal of Photochemistry and Photobiology A: Chemistry, 451, 115499.‏

Saadh, M. J., Mustafa, M. A., Hussein, N. M., Bansal, P., Kaur, H., Alubiady, M. H. S., ... & Margarian, S. (2024). Investigating the ability of BC2N nanotube to remove Eriochrome blue black from wastewater: A computational approach. Inorganic Chemistry Communications, 163, 112311.‏

Shakir, O. M., Abdulla, K. K., Mustafa, A. A., & Mustafa, M. A. (2019). Investigation of the presence of parasites that contaminate some fruits and vegetables in the Samarra City in Iraq. Plant Archives, 19, 1184-1190.‏

Simoni, M., Baldrighi, N., Degola, F., Marchi, L., Marseglia, A., & Righi, F. (2020). Low doses of lactoferrin supplementation in weaning calves. Acta Fytotechnica et Zootechnica, 23, 58-66.

Tacoma, R., Fields, J., Ebenstein, D. B., Lam, Y. W., & Greenwood, S. L. (2016). Characterization of the bovine milk proteome in early lactation Holstein and Jersey breed of dairy cows. Journal of Proteomics, 130, 200-210.

Valluru, D., Mustafa, M. A., Jasim, H. Y., Srikanth, K., Raja Rao, M. V. L. N., & Sreedhar, P. S. S. (2023, March). An efficient classroom teaching learning method using augmented reality. In 2023 9th International Conference on Advanced Computing and Communication Systems (ICACCS) (Vol. 1, pp. 300-303). IEEE.‏

Yaseen, A. H., Khalaf, A. T., & Mustafa, M. A. (2023). Lung cancer data analysis for finding gene expression. African Journal of Biological Sciences, 5(3), 119-130.