Evaluation of the Antimycobacterial efficacy of Silver, Gold and Bimetallic Nanoparticles synthesized using Indian medicinal plants

Gopinath; Gowsalya; Elanchezhiyan

doi:10.25163/primeasia.2120218

Multidisciplinary research and review journal | Online ISSN 3064-9870

RESEARCH ARTICLE (Open Access)

Previous Next Contents Vol 2 (1)

Evaluation of the Antimycobacterial efficacy of Silver, Gold and Bimetallic Nanoparticles synthesized using Indian medicinal plants

Gopinath Ramalingam^1*, Gowsalya Saminathan², Elanchezhiyan Manickan³

+ Author Affiliations

Journal of Primeasia 2 (1) 1-8 https://doi.org/10.25163/primeasia.2120218

Submitted: 23 May 2021 Revised: 21 July 2021 Published: 23 July 2021

Abstract

Background: Tuberculosis (TB), caused by the bacterium Mycobacterium tuberculosis (MTB), is the second most fatal infectious disease after AIDS. The rise of multi- and extensively drug-resistant TB poses a significant global health threat, necessitating novel therapeutic strategies. Nanomaterials have emerged as promising tools in medicine, diagnosis, and treatment. Traditional medicinal plants have long been used to treat TB, and this study investigates the anti-mycobacterial activity and cytotoxicity of nanoparticles synthesized from Indian medicinal plants. Methods: Silver (AgNPs), gold (AuNPs), and bimetallic (AuNPs-AgNPs) nanoparticles were synthesized using extracts from Andrographis paniculata, Acalypha indica, and Aloe vera. The anti-TB activity of these nanoparticles was evaluated using the Microplate Alamar Blue Assay at concentrations of 2, 4, 8, 16, 32, 64, and 128 μg/mL. Cytotoxicity was assessed on the Vero cell line using the MTT assay. Results: The bimetallic nanoparticles (AuNPs-AgNPs) demonstrated superior anti-TB activity compared to the mono-metallic nanoparticles. Among the mono-metallic nanoparticles, gold nanoparticles showed better efficacy than silver nanoparticles. A. indica-derived Au-AgNPs exhibited the most potent anti-TB activity, with the lowest minimum inhibitory concentration (MIC) and no cytotoxicity at effective concentrations. A. paniculata nanoparticles were moderately effective, while A. vera nanoparticles showed the least efficacy against MTB. Conclusion: A. indica appears to be the most promising candidate for further study due to its potent anti-TB efficacy and lack of cytotoxicity. A. paniculata also shows potential, whereas A. vera demonstrates minimal effectiveness against MTB. This study suggests that nanoparticle-based therapy could be a viable alternative for TB treatment.

Keywords: Tuberculosis, H37Rv, Andrographis paniculata, Acalypha indica and Aloevera.

References

Arora, N., Thangavelu, K., & Karanikolos, G. N. (2020). Bimetallic nanoparticles for antimicrobial applications. Frontiers in Chemistry, 8, 412.

Azam, A., et al. (2020). Bimetallic nanoparticles for antimicrobial applications: A review. Applied Surface Science, 529, 147024.

Barabadi, H., & Shaterian, H. R. (2018). The role of silver nanoparticles in antimicrobial activities: A review. Journal of Nanostructure in Chemistry, 8(2), 239-256.

Barabadi, H., et al. (2018). The role of silver nanoparticles in antimicrobial activities: A review. Journal of Nanostructure in Chemistry, 8(2), 239-256.

Bhattacharya, D., & Kundu, P. (2018). Synergistic antibacterial activity of bimetallic gold-silver nanoparticles: A new approach to overcome bacterial resistance. Materials Science and Engineering: C, 93, 243-253.

Choi, O., & Hu, Z. (2008). Nanosilver particles: Toxicity to human cells and the environment. Science, 319(5866), 1010-1014. https://doi.org/10.1126/science.1152473

El-Batal, A. I., Abd Elkodous, M., El-Sayyad, G. S., Al-Hazmi, N. E., Gobara, M., & Baraka, A. (2020). Gum Arabic polymer-stabilized and Gamma rays-assisted synthesis of bimetallic silver-gold nanoparticles: Powerful antimicrobial and antibiofilm activities against pathogenic microbes isolated from diabetic foot patients. International Journal of Biological Macromolecules, 165, 169-186.

El-Batal, A. I., et al. (2020). Gum Arabic polymer-stabilized and Gamma rays-assisted synthesis of bimetallic silver-gold nanoparticles: Powerful antimicrobial and antibiofilm activities against pathogenic microbes isolated from diabetic foot patients. International Journal of Biological Macromolecules, 165, 169-186.

El-Sayed, I. H., et al. (2005). Selective laser photoheating of gold nanoparticles. Journal of Physical Chemistry B, 109(18), 6869-6877.

Fanoro, O. T., & Oluwafemi, O. S. (2020). Bactericidal antibacterial mechanism of plant synthesized silver, gold and bimetallic nanoparticles. Pharmaceutics, 12(11), 1044.

Ganjalikhani-Hakemi, M., & Baghery, S. (2020). A comprehensive review on the toxicity of metal and metal oxide nanoparticles. Journal of Nanobiotechnology, 18(1), 122.

Ghosh, S., & Kumar, R. (2018). Synergistic effects of bimetallic nanoparticles: Advances and applications. Journal of Nanoparticle Research, 20, 123. https://doi.org/10.1007/s11051-018-4206-4

Huang, X., & El-Sayed, M. A. (2006). Gold nanoparticles: Optical properties and implementations in cancer diagnosis and photothermal therapy. Journal of Advanced Research, 1(1), 13-28.

Jain, P. K., et al. (2008). Noble metal nanostructures for biosensing and imaging. Chemical Reviews, 110(1), 614-637.

Jain, P. K., Huang, X., El-Sayed, I. H., & El-Sayed, M. A. (2008). Noble metal nanostructures for biosensing and imaging. Chemical Reviews, 110(1), 614-637.

Kesharwani, P., & Jain, N. K. (2014). Nanoparticle-based drug delivery systems: Perspectives and prospects. Journal of Nanomedicine & Nanotechnology, 5(2), 238-248. https://doi.org/10.4172/2157-7439.1000238

Kesharwani, P., et al. (2020). Bimetallic nanoparticles: Synthesis, characterization, and applications. Materials Science and Engineering: C, 116, 111243.

Kesharwani, P., Gajbhiye, V., & Jain, N. K. (2020). Bimetallic nanoparticles: Synthesis, characterization, and applications. Materials Science and Engineering: C, 116, 111243.

Khanna, P., & Ghosh, S. (2018). Synergistic effect of gold and silver nanoparticles on antimicrobial activity. Journal of Nanoscience and Nanotechnology, 18(8), 5467-5475.

Khedher, N. B., & Bouraoui, A. (2020). Plant-based synthesis of metal nanoparticles: Applications and perspectives. Journal of Nanotechnology, 2020, 1-17. https://doi.org/10.1155/2020/8507169

Kumar, V., & Yadav, S. K. (2009). Plant-mediated synthesis of silver and gold nanoparticles and their applications. Journal of Chemical Technology & Biotechnology, 84(2), 151-157.

McCarthy, T., & Byrne, C. (2020). Antimicrobial and cytotoxic properties of biogenic nanoparticles from plant extracts. Biotechnology Reports, 27, e00487.

Mucfherjee, S., & Pal, A. (2020). Enhanced antibacterial and antitumor activity of gold nanoparticles. Journal of Nanoscience and Nanotechnology, 20(7), 4024-4032.

Nallamuthu, N., & Sivaraman, K. (2016). Synthesis and characterization of bimetallic Au–Ag nanoparticles for antimicrobial applications. Journal of Nanomaterials, 2016, 9.

Nithya, P., & Sundrarajan, M. (2020). Ionic liquid functionalized biogenic synthesis of AgAu bimetal doped CeO2 nanoparticles from Justicia adhatoda for pharmaceutical applications: Antibacterial and anti-cancer activities. Journal of Photochemistry and Photobiology B: Biology, 202, 111706.

Padua, A., & Mota, S. (2017). Bimetallic nanoparticles: Properties and applications. Materials Science and Engineering: R: Reports, 116, 1-27. https://doi.org/10.1016/j.mser.2017.05.001

Park, J., An, K., & Park, J. (2004). Ultra-sensitive detection of biomolecules using gold nanoparticles and antibodies. Nanotechnology, 15(2), 234-243.

Rai, M., Yadav, A., & Gade, A. (2009). Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 27(1), 76-83. https://doi.org/10.1016/j.biotechadv.2008.07.002

Ramachandran, A., & Muthusamy, K. (2020). Biocompatibility and cytotoxicity of gold and silver nanoparticles: A review. International Journal of Nanomedicine, 15, 123-135.

Ranjbar, M., & Moosavi, S. M. (2019). Nanoparticles in medicine and therapy. Journal of Nanomedicine, 12, 1-10. https://doi.org/10.2147/JN.S177238

Reddy, L. A., & Reddy, G. H. (2014). X-ray diffraction study of metal nanoparticles. Journal of Nanoscience and Nanotechnology, 14(1), 438-442.

Sangeetha, N., & Reddy, M. S. (2015). Evaluation of antimicrobial activities of gold nanoparticles synthesized using leaf extracts of Azadirachta indica. Journal of Nanoparticle Research, 17(10), 394.

Sharma, V. K., Yngard, R. A., & Lin, Y. (2009). Silver nanoparticles: Green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science, 145(1-2), 83-96. https://doi.org/10.1016/j.cis.2008.09.002

Zhang, Q., & Chen, C. (2018). Nanomaterials as drug delivery systems. Journal of Nanoscience and Nanotechnology, 18(12), 8715-8731. https://doi.org/10.1166/jnn.2018.15244

Zhao, W., & Huang, X. (2019). Biogenic gold and silver nanoparticles: Synthesis, properties, and biomedical applications. Nanoscale Advances, 1(11), 4483-4503.

Zumla, A., Raviglione, M., Hafner, R., & von Reyn, C. F. (2013). Tuberculosis. The Lancet, 382(9909), 1479-1499. https://doi.org/10.1016/S0140-6736(13)62173-3

PDF
Abstract
Export Citation

View Dimensions

View Plumx

View Altmetric

Save

0
Citation

279
View

Share